Ventria Bioscience; Availability of Revised Environmental Assessment, With Consideration for an Additional Test Site in North Carolina, for Field Tests of Genetically Engineered Rice Expressing Lysozyme, 25522-25524 [05-9607]
Download as PDF
25522
Federal Register / Vol. 70, No. 92 / Friday, May 13, 2005 / Notices
sure someone is there to help you,
please call (202) 690–2817 before
coming.
Other Information: You may view
APHIS documents published in the
Federal Register and related
information on the Internet at https://
www.aphis.usda.gov/ppd/rad/
webrepor.html.
FOR FURTHER INFORMATION CONTACT: Dr.
Levis Handley, Biotechnology
Regulatory Services, APHIS, 4700 River
Road Unit 147, Riverdale, MD 20737–
1236; (301) 734–5721. To obtain copies
of the environmental assessment,
contact Ms. Ingrid Berlanger, at (301)
734–4885; e-mail
ingrid.e.berlanger@aphis.usda.gov. The
environmental assessment is also
available on the Internet at https://
www.aphis.usda.gov/brs/aphisdocs/
05_11701r_ea.pdf.
SUPPLEMENTARY INFORMATION: The
regulations in 7 CFR part 340,
‘‘Introduction of Organisms and
Products Altered or Produced Through
Genetic Engineering Which Are Plant
Pests or Which There Is Reason To
Believe Are Plant Pests,’’ regulate,
among other things, the introduction
(importation, interstate movement, or
release into the environment) of
organisms and products altered or
produced through genetic engineering
that are plant pests or that there is
reason to believe are plant pests. Such
genetically engineered organisms and
products are considered ‘‘regulated
articles.’’ A permit must be obtained or
a notification acknowledged before a
regulated article may be introduced into
the United States. The regulations set
forth the permit application
requirements and the notification
procedures for the importation,
interstate movement, and release into
the environment of a regulated article.
On October 28, 2004, the Animal and
Plant Health Inspection Service (APHIS)
received a permit application (APHIS
permit number 04–302–01r) from
Ventria Bioscience, Sacramento, CA, for
a permit for a confined field test of rice
(Oryza sativa) plants genetically
engineered to express a gene coding for
the protein lactoferrin, rice line LF164–
12. According to the permit application,
the field test would be conducted in
Scott County, MO. On February 23,
2005, APHIS published a notice in the
Federal Register (70 FR 8763, Docket
No. 05–006–1), announcing the
availability of an environmental
assessment (EA) for the proposed field
test and soliciting public comments for
30 days. This 30-day comment period
closed on March 25, 2005. During the
30-day comment period, APHIS
VerDate jul<14>2003
15:59 May 12, 2005
Jkt 205001
received 309 comments. Comments
were received from rice growers, rice
marketing and processing groups,
agricultural support businesses,
consumer groups, university
professionals, private individuals,
industry trade organizations, large rice
purchasers, growers of crops other than
rice, and Federal, State and local
government representatives.
On April 27, 2005, while APHIS was
evaluating these comments, we received
a request from Ventria Biosciences to
plant rice line LF164–12 in a second site
in Washington County, NC (APHIS
permit number 05–117–01r). At this
time, Ventria Biosciences has not
withdrawn its application to conduct a
field test in Scott County, MO. However,
it is likely that conducting a field test
for this growing season is not feasible
due to climatic factors in this location.
Because APHIS has not yet considered
all of the comments associated with the
earlier EA and the issues raised in North
Carolina are similar to those in
Missouri, APHIS has amended the EA to
evaluate the issues in North Carolina as
well as Missouri. These are covered in
Appendices V and VI. In addition to
evaluating site-specific issues presented
by the North Carolina application, this
revised EA also corrects errors in the
original EA. These changes are
described in the summary of the EA.
APHIS is seeking comments on the
additional information provided in this
revised EA. We are particularly
interested in comments related to
Appendices V and VI that address
issues in North Carolina. APHIS will
consider all comments received during
the previous comment period (70 FR
8763, Docket No. 05–006–1) as well as
any new comments received during this
comment period (see DATES above). The
expanded EA will be open for public
comment for an additional 20 days.
The subject rice plants have been
genetically engineered, using microprojectile bombardment, to express
human lactoferrin protein. Expression of
the gene is controlled by the rice
glutelin 1 promoter, the rice glutelin 1
signal peptide, and the nos (nopaline
synthase) terminator sequence from
Agrobacterium tumefaciens. The gene is
expressed only in the endosperm. In
addition, the plants contain the coding
sequence for the gene hygromycin
phosphotransferase (hpt), an enzyme
which confers tolerance to the antibiotic
hygromycin. This gene is a selectable
marker that is only expressed during
plant cell culture and is not expressed
in any tissues of the mature plant.
Expression of the gene is controlled by
the rice glucanase 9 (Gns 9) promoter
and the Rice Alpha Amylase 1A
PO 00000
Frm 00002
Fmt 4703
Sfmt 4703
(RAmy1A) terminator. The genetically
engineered rice plants are considered
regulated articles under the regulations
in 7 CFR part 340 because they contain
gene sequences from plant pathogens.
The purpose of the field planting is
for pure seed production and for the
extraction of lactoferrin for a variety of
research and commercial products. The
planting will be conducted using
physical confinement measures. In
addition, the experimental protocols
and field plot design, as well as the
procedures for termination of the field
planting, are designed to ensure that
none of the subject rice plants persist in
the environment beyond the termination
of the experiments.
To provide the public with
documentation of APHIS’ review and
analysis of any potential environmental
impacts and plant pest risk associated
with the proposed confined field
planting of the subject rice plants, an
environment assessment (EA) has been
prepared. The EA was prepared in
accordance with (1) The National
Environmental Policy Act of 1969
(NEPA), as amended (42 U.S.C. 4321 et
seq.), (2) regulations of the Council on
Environmental Quality for
implementing the procedural provisions
of NEPA (40 CFR parts 1500–1508), (3)
USDA regulations implementing NEPA
(7 CFR part 1b), and (4) APHIS’ NEPA
Implementing Procedures (7 CFR part
372). Copies of the EA are available
from the individual listed under FOR
FURTHER INFORMATION CONTACT.
Done in Washington, DC, this 9th day of
May 2005.
Elizabeth E. Gaston,
Acting Administrator, Animal and Plant
Health Inspection Service.
[FR Doc. 05–9606 Filed 5–12–05; 8:45 am]
BILLING CODE 3410–34–P
DEPARTMENT OF AGRICULTURE
Animal and Plant Health Inspection
Service
[Docket No. 05–007–2]
Ventria Bioscience; Availability of
Revised Environmental Assessment,
With Consideration for an Additional
Test Site in North Carolina, for Field
Tests of Genetically Engineered Rice
Expressing Lysozyme
Animal and Plant Health
Inspection Service, USDA.
ACTION: Notice.
AGENCY:
SUMMARY: We are advising the public
that the Animal and Plant Health
Inspection Service has revised an
environmental assessment for confined
E:\FR\FM\13MYN1.SGM
13MYN1
Federal Register / Vol. 70, No. 92 / Friday, May 13, 2005 / Notices
field tests of rice plants genetically
engineered to express the protein
lysozyme and has included information
on an additional field test site. This
environmental assessment is available
for public review and comment.
DATES: We will consider all comments
that we receive on or before June 2,
2005.
You may submit comments
by either of the following methods:
• EDOCKET: Go to https://
www.epa.gov/feddocket to submit or
view public comments, access the index
listing of the contents of the official
public docket, and to access those
documents in the public docket that are
available electronically. Once you have
entered EDOCKET, click on the ‘‘View
Open APHIS Dockets’’ link to locate this
document.
• Postal Mail/Commercial Delivery:
Please send four copies of your
comment (an original and three copies)
to Docket No. 05–007–2, Regulatory
Analysis and Development, PPD,
APHIS, Station 3C71, 4700 River Road
Unit 118, Riverdale, MD 20737–1238.
Please state that your comment refers to
Docket No. 05–007–2.
Reading Room: You may read the
environmental assessment and any
comments that we receive in our
reading room. The reading room is
located in room 1141 of the USDA
South Building, 14th Street and
Independence Avenue SW.,
Washington, DC. Normal reading room
hours are 8 a.m. to 4:30 p.m., Monday
through Friday, except holidays. To be
sure someone is there to help you,
please call (202) 690–2817 before
coming.
Other Information: You may view
APHIS documents published in the
Federal Register and related
information on the Internet at https://
www.aphis.usda.gov/ppd/rad/
webrepor.html.
ADDRESSES:
Dr.
Levis Handley, Biotechnology
Regulatory Services, APHIS, 4700 River
Road Unit 147, Riverdale, MD 20737–
1236; (301) 734–5721. To obtain copies
of the environmental assessment,
contact Ms. Ingrid Berlanger, at (301)
734–4885; e-mail
ingrid.e.berlanger@aphis.usda.gov. The
environmental assessment is also
available on the Internet at https://
www.aphis.usda.gov/brs/aphisdocs/
05_11702r_ea.pdf.
FOR FURTHER INFORMATION CONTACT:
The
regulations in 7 CFR part 340,
‘‘Introduction of Organisms and
Products Altered or Produced Through
Genetic Engineering Which Are Plant
SUPPLEMENTARY INFORMATION:
VerDate jul<14>2003
15:59 May 12, 2005
Jkt 205001
Pests or Which There Is Reason To
Believe Are Plant Pests,’’ regulate,
among other things, the introduction
(importation, interstate movement, or
release into the environment) of
organisms and products altered or
produced through genetic engineering
that are plant pests or that there is
reason to believe are plant pests. Such
genetically engineered organisms and
products are considered ‘‘regulated
articles.’’ A permit must be obtained or
a notification acknowledged before a
regulated article may be introduced into
the United States. The regulations set
forth the permit application
requirements and the notification
procedures for the importation,
interstate movement, and release into
the environment of a regulated article.
On October 28, 2004, the Animal and
Plant Health Inspection Service (APHIS)
received a permit application (APHIS
permit number 04–309–01r) from
Ventria Bioscience, Sacramento, CA, for
a permit for a confined field test of rice
(Oryza sativa) plants genetically
engineered to express a gene coding for
the protein lysozyme, rice line LZ159–
53. According to the permit application,
the field test would be conducted in
Scott County, MO. On February 23,
2005, APHIS published a notice in the
Federal Register (70 FR 8762–8763,
Docket No. 05–007–1), announcing the
availability of an environmental
assessment (EA) for the proposed field
test and soliciting public comments for
30 days. This 30-day comment period
closed on March 25, 2005. During the
30-day comment period, APHIS
received 243 comments. Comments
were received from rice growers, rice
marketing and processing groups,
agricultural support businesses,
consumer groups, university
professionals, private individuals,
industry trade organizations, large rice
purchasers, growers of crops other than
rice, and Federal, State and local
government representatives.
On April 27, 2005, while APHIS was
evaluating these comments, we received
a request from Ventria Biosciences to
plant rice line LF164–12 in a second site
in Washington County, NC (APHIS
permit number 05–117–02r). At this
time, Ventria Biosciences has not
withdrawn its application to conduct a
field test in Scott County, MO. However,
it is likely that conducting a field test
for this growing season is not feasible
due to climatic factors in this location.
Because APHIS has not yet considered
all of the comments associated with the
earlier EA and the issues raised in North
Carolina are similar to those in
Missouri, APHIS has amended the EA to
evaluate the issues in North Carolina as
PO 00000
Frm 00003
Fmt 4703
Sfmt 4703
25523
well as Missouri. These are covered in
Appendices V and VI. In addition to
evaluating site-specific issues presented
by the North Carolina application, this
revised EA also corrects errors in the
original EA. These changes are
described in the summary of the EA.
APHIS is seeking comments on the
additional information provided in this
revised EA. We are particularly
interested in comments related to
Appendices V and VI that address
issues in North Carolina. APHIS will
consider all comments received during
the previous comment period (70 FR
8762–8763, Docket No. 05–007–1) as
well as any new comments received
during this comment period (see DATES
above). The expanded EA will be open
for public comment for an additional 20
days.
The subject rice plants have been
genetically engineered, using microprojectile bombardment, to express
human lysozyme protein. Expression of
the gene is controlled by the rice
glutelin 1 promoter, the rice glutelin 1
signal peptide, and the nos (nopaline
synthase) terminator sequence from
Agrobacterium tumefaciens. The gene is
expressed only in the endosperm. In
addition, the plants contain the coding
sequence for the gene hygromycin
phosphotransferase (hpt), an enzyme
which confers tolerance to the antibiotic
hygromycin. This gene is a selectable
marker that is only expressed during
plant cell culture and is not expressed
in any tissues of the mature plant.
Expression of the gene is controlled by
the rice glucanase 9 (Gns 9) promoter
and the Rice Alpha Amylase 1A
(RAmy1A) terminator. The genetically
engineered rice plants are considered
regulated articles under the regulations
in 7 CFR part 340 because they contain
gene sequences from plant pathogens.
The purpose of the field planting is
for pure seed production and for the
extraction of lysozyme for a variety of
research and commercial products. The
planting will be conducted using
physical confinement measures. In
addition, the experimental protocols
and field plot design, as well as the
procedures for termination of the field
planting, are designed to ensure that
none of the subject rice plants persist in
the environment beyond the termination
of the experiments.
To provide the public with
documentation of APHIS’ review and
analysis of any potential environmental
impacts and plant pest risk associated
with the proposed confined field
planting of the subject rice plants, an
environment assessment (EA) has been
prepared. The EA was prepared in
accordance with (1) The National
E:\FR\FM\13MYN1.SGM
13MYN1
25524
Federal Register / Vol. 70, No. 92 / Friday, May 13, 2005 / Notices
Environmental Policy Act of 1969
(NEPA), as amended (42 U.S.C. 4321 et
seq.), (2) regulations of the Council on
Environmental Quality for
implementing the procedural provisions
of NEPA (40 CFR parts 1500–1508), (3)
USDA regulations implementing NEPA
(7 CFR part 1b), and (4) APHIS’ NEPA
Implementing Procedures (7 CFR part
372). Copies of the EA are available
from the individual listed under FOR
FURTHER INFORMATION CONTACT.
Done in Washington, DC, this 9th day of
May 2005.
Elizabeth E. Gaston,
Acting Administrator, Animal and Plant
Health Inspection Service.
[FR Doc. 05–9607 Filed 5–12–05; 8:45 am]
BILLING CODE 3410–34–P
DEPARTMENT OF AGRICULTURE
Forest Service
Cibola National Forest; New Mexico;
Canadian River Tamarisk Control
Project
Forest Service, USDA.
Notice of intent to prepare an
environmental impact statement.
AGENCY:
ACTION:
SUMMARY: The Department of
Agriculture, Forest Service has initiated
the process to prepare an Environmental
Impact Statement for the Canadian River
Tamarisk Control Project on the Cibola
National Forest, Kiowa National
Grassland. The proposed action would
restore the hydrologic function of the
Canadian River by eradicating tamarisk
(salt cedar) along the river corridor and
tributaries, covering 16 miles
(approximately 540 acres) that occur on
Federal administrative lands. This
proposal includes the use of a helicopter
to aerially apply the herbicide imazapyr
(common trade names Arsenal and
Habitat) along with an approved
surfactant and drift control agent, and/
or use mechanical treatments and
backpack sprayers to apply the same
herbicide to cut stumps in designated
areas. The objective is to eradicate
tamarisk from this section of the
Canadian River and promote the reestablishment of native riparian
vegetation and habitat conditions for
wildlife.
Salt cedar has actively invaded the
riparian area along the Canadian River,
replacing native plants and wildlife.
The Canadian River supplies irrigation
water to thousands of acres of
agriculture land, provides for
recreational opportunities, and is home
to several indigenous wildlife species.
Tamarisk is listed by both the State of
VerDate jul<14>2003
15:59 May 12, 2005
Jkt 205001
New Mexico Department of Agriculture
and the Federal government as a
noxious weed. The State of New Mexico
has identified tamarisk as a species that
is causing an ecological crisis in several
river systems throughout the state,
including the Canadian River. Land
owners both above and below the
National Grassland segment of the
Canadian River are in the process of
treating their lands to control tamarisk
using the same types of treatment
methods. This effort would be
coordinated with those other treatment
efforts within this watershed.
Tamarisk is known to cause a change
in ecological conditions that tend to
eliminate native species and reduce
water delivery, due to its ability to
transpire large amounts of water during
the growing season. Herbicide
treatments have been shown to be an
effective and efficient method for
eradicating tamarisk and returning the
riparian habitat to a healthy functioning
ecosystem that is beneficial to both the
biotic and human environments.
The Canadian River Canyon has been
identified as an inventoried roadless
area. The Canadian River also has
eligibility status as a scenic river under
the Wild and Scenic Rivers Act, and
outstanding remarkable values would be
protected until a decision is made on
the future use of the river and adjacent
lands or until an action is taken by
Congress to designate the river as such.
DATES: Comments concerning the scope
of the analysis must be received by June
15, 2005. The draft environmental
impact statement is expected to be
published in October, 2005, and the
final environmental impact statement is
expected in December 2005.
ADDRESSES: Send written comments to
Deborah Walker, NEPA Coordinator;
Cibola National Forest; 2113 Osuna
Road NE; Albuquerque, NM 87113 or
FAX to 505–346–3901. Copies of the
proposed action, project location map,
or the Environmental Impact Statement,
when available, may be obtained from
the Cibola National Forest; 2113 Osuna
Road, NE; Albuquerque, NM 87113; or
from the Kiowa National Grassland; 714
Main Street; Clayton, NM 88415, or
from the Forest Web site at
www.fs.fed.us/r3/cibola/projects/
index.shtml.
For
further information, mail
correspondence to Deborah Walker,
NEPA Coordinator; Cibola National
Forest; 2113 Osuna Road NE;
Albuquerque, NM 87113 or phone 505–
346–3888.
SUPPLEMENTARY INFORMATION:
FOR FURTHER INFORMATION CONTACT:
PO 00000
Frm 00004
Fmt 4703
Sfmt 4703
Purpose and Need for Action
The purpose of the Canadian River
Tamarisk Control project is to:
1. Restore the hydrologic function of
the Canadian River by eradicating
tamarisk along the river corridor and
tributaries using methods that have
proven to be both safe and effective.
2. Re-establish native riparian species
and the habitat it provides for wildlife.
3. Coordinate activities with adjacent
landowners both above and below the
Kiowa National Grassland boundary in
an effort to control tamarisk within the
entire Canadian River corridor.
Proposed Action
The Cibola National Forest, Kiowa
National Grassland, proposes to apply
imazapyr using aerial and backpack
spray application methods to 16 miles
of the Canadian River and tributaries.
Aerial application would be made using
a helicopter with spray boom on an
estimated 380 acres where the tamarisk
is very dense or where the stands are
inaccessible to vehicles. Backpack spray
(hand treatment) would be used after
tamarisk has been cut with either
chainsaws or tractor, and the herbicide
is applied to the cut stump, or the
herbicide is applied over the top of
stems as a foliar application (estimated
160 acres). Backpack spray treatments
would occur on Forest Service
administered lands near the Mills
Canyon campground and in areas where
there is a predominance of native
riparian vegetation that are accessible by
existing roads or trails. A nonionic
surfactant and drift control agent
(vegetable oil based) would be mixed
with imazapyr in order to improve
effectiveness. An estimated 1 pound of
acid equivalent of active ingredient
would be applied per acre. Treatments
would be applied between late July and
late September. Re-treatments would be
applied on a limited basis as needed to
control re-sprouting tamarisk for up to
five years following initial treatment.
Dead trees would remain in place for a
minimum of two growing seasons after
which hazardous trees would be
removed within the campground or
other accessible places as needed for
public safety.
Rehabilitation efforts following
treatment would include replanting
with native riparian species (i.e.,
cottonwood, willow, or maple) and
reseeding areas disturbed by equipment
with native grasses in order to stabilize
soil and provide ground cover, as
needed.
Resource protection measures that
would be implemented as part of this
proposal include protection of known
E:\FR\FM\13MYN1.SGM
13MYN1
Agencies
[Federal Register Volume 70, Number 92 (Friday, May 13, 2005)]
[Notices]
[Pages 25522-25524]
From the Federal Register Online via the Government Printing Office [www.gpo.gov]
[FR Doc No: 05-9607]
-----------------------------------------------------------------------
DEPARTMENT OF AGRICULTURE
Animal and Plant Health Inspection Service
[Docket No. 05-007-2]
Ventria Bioscience; Availability of Revised Environmental
Assessment, With Consideration for an Additional Test Site in North
Carolina, for Field Tests of Genetically Engineered Rice Expressing
Lysozyme
AGENCY: Animal and Plant Health Inspection Service, USDA.
ACTION: Notice.
-----------------------------------------------------------------------
SUMMARY: We are advising the public that the Animal and Plant Health
Inspection Service has revised an environmental assessment for confined
[[Page 25523]]
field tests of rice plants genetically engineered to express the
protein lysozyme and has included information on an additional field
test site. This environmental assessment is available for public review
and comment.
DATES: We will consider all comments that we receive on or before June
2, 2005.
ADDRESSES: You may submit comments by either of the following methods:
EDOCKET: Go to https://www.epa.gov/feddocket to submit or
view public comments, access the index listing of the contents of the
official public docket, and to access those documents in the public
docket that are available electronically. Once you have entered
EDOCKET, click on the ``View Open APHIS Dockets'' link to locate this
document.
Postal Mail/Commercial Delivery: Please send four copies
of your comment (an original and three copies) to Docket No. 05-007-2,
Regulatory Analysis and Development, PPD, APHIS, Station 3C71, 4700
River Road Unit 118, Riverdale, MD 20737-1238. Please state that your
comment refers to Docket No. 05-007-2.
Reading Room: You may read the environmental assessment and any
comments that we receive in our reading room. The reading room is
located in room 1141 of the USDA South Building, 14th Street and
Independence Avenue SW., Washington, DC. Normal reading room hours are
8 a.m. to 4:30 p.m., Monday through Friday, except holidays. To be sure
someone is there to help you, please call (202) 690-2817 before coming.
Other Information: You may view APHIS documents published in the
Federal Register and related information on the Internet at https://
www.aphis.usda.gov/ppd/rad/webrepor.html.
FOR FURTHER INFORMATION CONTACT: Dr. Levis Handley, Biotechnology
Regulatory Services, APHIS, 4700 River Road Unit 147, Riverdale, MD
20737-1236; (301) 734-5721. To obtain copies of the environmental
assessment, contact Ms. Ingrid Berlanger, at (301) 734-4885; e-mail
ingrid.e.berlanger@aphis.usda.gov. The environmental assessment is also
available on the Internet at https://www.aphis.usda.gov/brs/aphisdocs/
05_11702r_ea.pdf.
SUPPLEMENTARY INFORMATION: The regulations in 7 CFR part 340,
``Introduction of Organisms and Products Altered or Produced Through
Genetic Engineering Which Are Plant Pests or Which There Is Reason To
Believe Are Plant Pests,'' regulate, among other things, the
introduction (importation, interstate movement, or release into the
environment) of organisms and products altered or produced through
genetic engineering that are plant pests or that there is reason to
believe are plant pests. Such genetically engineered organisms and
products are considered ``regulated articles.'' A permit must be
obtained or a notification acknowledged before a regulated article may
be introduced into the United States. The regulations set forth the
permit application requirements and the notification procedures for the
importation, interstate movement, and release into the environment of a
regulated article.
On October 28, 2004, the Animal and Plant Health Inspection Service
(APHIS) received a permit application (APHIS permit number 04-309-01r)
from Ventria Bioscience, Sacramento, CA, for a permit for a confined
field test of rice (Oryza sativa) plants genetically engineered to
express a gene coding for the protein lysozyme, rice line LZ159-53.
According to the permit application, the field test would be conducted
in Scott County, MO. On February 23, 2005, APHIS published a notice in
the Federal Register (70 FR 8762-8763, Docket No. 05-007-1), announcing
the availability of an environmental assessment (EA) for the proposed
field test and soliciting public comments for 30 days. This 30-day
comment period closed on March 25, 2005. During the 30-day comment
period, APHIS received 243 comments. Comments were received from rice
growers, rice marketing and processing groups, agricultural support
businesses, consumer groups, university professionals, private
individuals, industry trade organizations, large rice purchasers,
growers of crops other than rice, and Federal, State and local
government representatives.
On April 27, 2005, while APHIS was evaluating these comments, we
received a request from Ventria Biosciences to plant rice line LF164-12
in a second site in Washington County, NC (APHIS permit number 05-117-
02r). At this time, Ventria Biosciences has not withdrawn its
application to conduct a field test in Scott County, MO. However, it is
likely that conducting a field test for this growing season is not
feasible due to climatic factors in this location. Because APHIS has
not yet considered all of the comments associated with the earlier EA
and the issues raised in North Carolina are similar to those in
Missouri, APHIS has amended the EA to evaluate the issues in North
Carolina as well as Missouri. These are covered in Appendices V and VI.
In addition to evaluating site-specific issues presented by the North
Carolina application, this revised EA also corrects errors in the
original EA. These changes are described in the summary of the EA.
APHIS is seeking comments on the additional information provided in
this revised EA. We are particularly interested in comments related to
Appendices V and VI that address issues in North Carolina. APHIS will
consider all comments received during the previous comment period (70
FR 8762-8763, Docket No. 05-007-1) as well as any new comments received
during this comment period (see DATES above). The expanded EA will be
open for public comment for an additional 20 days.
The subject rice plants have been genetically engineered, using
micro-projectile bombardment, to express human lysozyme protein.
Expression of the gene is controlled by the rice glutelin 1 promoter,
the rice glutelin 1 signal peptide, and the nos (nopaline synthase)
terminator sequence from Agrobacterium tumefaciens. The gene is
expressed only in the endosperm. In addition, the plants contain the
coding sequence for the gene hygromycin phosphotransferase (hpt), an
enzyme which confers tolerance to the antibiotic hygromycin. This gene
is a selectable marker that is only expressed during plant cell culture
and is not expressed in any tissues of the mature plant. Expression of
the gene is controlled by the rice glucanase 9 (Gns 9) promoter and the
Rice Alpha Amylase 1A (RAmy1A) terminator. The genetically engineered
rice plants are considered regulated articles under the regulations in
7 CFR part 340 because they contain gene sequences from plant
pathogens.
The purpose of the field planting is for pure seed production and
for the extraction of lysozyme for a variety of research and commercial
products. The planting will be conducted using physical confinement
measures. In addition, the experimental protocols and field plot
design, as well as the procedures for termination of the field
planting, are designed to ensure that none of the subject rice plants
persist in the environment beyond the termination of the experiments.
To provide the public with documentation of APHIS' review and
analysis of any potential environmental impacts and plant pest risk
associated with the proposed confined field planting of the subject
rice plants, an environment assessment (EA) has been prepared. The EA
was prepared in accordance with (1) The National
[[Page 25524]]
Environmental Policy Act of 1969 (NEPA), as amended (42 U.S.C. 4321 et
seq.), (2) regulations of the Council on Environmental Quality for
implementing the procedural provisions of NEPA (40 CFR parts 1500-
1508), (3) USDA regulations implementing NEPA (7 CFR part 1b), and (4)
APHIS' NEPA Implementing Procedures (7 CFR part 372). Copies of the EA
are available from the individual listed under FOR FURTHER INFORMATION
CONTACT.
Done in Washington, DC, this 9th day of May 2005.
Elizabeth E. Gaston,
Acting Administrator, Animal and Plant Health Inspection Service.
[FR Doc. 05-9607 Filed 5-12-05; 8:45 am]
BILLING CODE 3410-34-P