Current through Register Vol. 56, No. 24, December 18, 2024
(a) For
regulatory samples that are to be analyzed for microbiological parameters to
demonstrate compliance with the drinking water program:
1. The requirements of (c) below shall be
satisfied;
2. Sample containers,
preservation techniques, and holding times shall satisfy the requirements under
N.J.A.C.
7:18-9.4(b)1 and Table 9.1;
and
3. Collection, handling,
analysis and preservation of drinking water samples for compliance with the
statutes listed at
N.J.A.C.
7:18-1.1(c)1 and 7 shall
adhere to the sampling identification, and transfer procedures described in the
latest edition of Standard Methods approved by the USEPA. If there is any
conflict between the collection, handling and preservation requirements in
Standard Methods and the corresponding requirements in this subchapter, the
requirements in Standard Methods shall control.
(b) For regulatory samples that are to be
analyzed for microbiological parameters to demonstrate compliance with the
water pollution program:
1. The requirements
of (c) below shall be satisfied; and
2. Sample containers, preservation
techniques, and holding times shall satisfy the requirements under
N.J.A.C.
7:18-9.4(c) and Table
9.2.
(c) In addition to
the requirements of Table 9.1 or 9.2, as applicable, the requirements listed in
(c)1 through 13 below shall be satisfied for samples to be analyzed for one or
more microbiological parameters. The requirements listed in (c)1 through 13
below are incorporated from the USEPA's "Microbiological Methods for Monitoring
the Environment, Water and Wastes," EPA-600/8-78-017. If there are any
conflicts between the USEPA publication and (c)1 through 13 below, the USEPA
publication shall control.
1. The sample
volume shall be at least 100 mL;
2.
The sample container shall not be filled completely, to allow adequate air
space for mixing;
3. The sample
container shall have a capacity of at least 120 mL. The sample container shall
be one of the following:
i. A wide-mouthed
hard glass and leakproof sample bottle;
ii. A plastic sample bottle or container with
a leakproof cap; or
iii. A
pre-sterilized plastic bag;
4. Glass-stoppered bottles shall be stored so
that they are protected from contamination by dust and the glass stoppers shall
be covered with either aluminum foil or kraft paper;
5. Caps shall have leakproof nontoxic liners
that are capable of withstanding repeated sterilizations, at temperatures of
121 degrees Celsius sustained for 30 minutes per sterilization;
6. Sample containers shall have sodium
thiosulfate (0.1 mL of 10 percent (weight/volume) solution per 120 mL capacity)
added prior to sterilization;
7.
When collecting samples known to contain heavy metals, add ethylenediamine
tetra acetic acid (EDTA) (0.3 mL of a 15% (weight/volume) solution per 120 mL
capacity bottle) to the sample container prior to sterilization;
8. The collector shall complete a sample
analysis request form immediately after collection. The collector shall state
the following on the form:
i. That sterilized
containers with preservative were used for sampling;
ii. The collector's name and
affiliation;
iii. Name and
identification number of the environmental laboratory analyzing the
sample;
iv. Sample location and
type;
v. Date and time of
collection;
vi. Chlorine residual
results, if applicable;
vii.
Preservatives or preservation conditions used;
viii. DSAMs to be performed; and
ix. Collector's signature and any
remarks.
9. Unless the
requirements of (c)13 below are satisfied, a chain-of-custody form shall be
completed. The form shall provide space for the sample analysis request
information listed in (c)8 above. The following chain-of-custody procedures
shall be employed, and the following information shall be recorded by each
person who collects or handles a regulatory sample:
i. Use tie-on or affixed labels with sample
identification to label the sample; and
ii. After the sample has been collected, the
collector shall write the following information on the chain-of-custody form:
(1) The information required under (c)8i
through viii above;
(2) Signature,
date and time of chain-of-custody transfers; and
(3) Number of containers;
10. When sending
samples by mail or private shipping service, the collector shall complete the
chain-of-custody form before shipping, and place it into the shipping
container. The container shall have a numbered custody seal;
11. Samples shall be stored in iced coolers
at four degrees Celsius during transit to the certified environmental
laboratory and refrigerated upon delivery until such analyses can be
performed;
12. A certified
environmental laboratory shall not accept a sample unless it is properly
labelled, and for which assurance is given that the sample has been collected,
preserved, processed, stored and transported in a manner that will assure the
identity of the sample and that the sample is sufficiently stable to be used in
the requested tests or analyses; and
13. A formal chain-of-custody procedure is
not required if:
i. The collector and the
analyst are the same person; and
ii. The collector enters in the field log
book all of the information required under (c)8 above.