(c) The laboratory shall follow the following requirements when reporting results of acute toxicity tests:
1. The laboratory shall complete a sample report immediately after collecting either dilution water or effluent samples. The laboratory shall make the report either as a separate form or as an entry in a bound logbook. The laboratory shall include the sample report data in its report of the toxicity test results to the Department. The laboratory shall include the following information in the sample report:
i. The sampling location;
ii. Date and time of collection;
iii. Total residual chlorine level in the sample;
iv. The type of sample (composite or grab);
v. The material sampled;
vi. The collector's name; and
vii. Any pertinent comments.
2. The laboratory shall include the following types of information when reporting results of an acute toxicity test:
i. The name of the test, the investigator(s), and the laboratory;
ii. The date on which the test began;
iii. The name of NJPDES permittee, its location, its NJPDES permit number, and the Discharge Serial Number;
iv. The name of the receiving water body;
v. A detailed description of the effluent, including the sampling point, date and time of collection, known physical and chemical properties, and variability;
vi. A detailed description of the dilution water source, the sample location, the date and time of sample collection, the tide stage (if applicable), the dilution water chemical characteristics, and any pretreatment;
vii. A description of acute toxicity test method(s) used, including:
(1) The test protocol, a definition of the adverse effect (death, immobility, etc.) used in the test, a description of the test chambers used (including the depth and volume of test solution), the number of test organisms per replicate treatment, the number of replicate treatments, and the loading rate of the test organisms. In flow-through toxicity tests the report shall also include the number of water volume exchanges per 24 hours in each test chamber;
(2) Detailed information about the test organisms, including the scientific name, mean and ranges of length and weight (where appropriate), age, life stage, source, previous history (if known), observed disease, treatments (if any), record of acclimation procedure, and observations on behavior during the test;
(3) A description of any aeration or salinity adjustments performed on test solutions before or during the test;
(4) The methods used for all chemical analyses;
(5) The mean and range of the acclimation temperature, test temperature, and salinity; and
(6) Any deviations from the test method(s);
viii. The test results, including the following:
(1) The results of all chemical analyses of the effluent and dilution water;
(2) A daily record of the number and percentages of organisms in each test chamber, including the control treatments, that died or showed the selected effect;
(3) A summary of general observations of other effects of symptoms of toxicity observed during the test;
(4) The LC[50] or EC[50] value with specified exposure time and the 95 percent confidence limits. If the highest effluent concentration did not kill or affect 50 percent or more of the test organisms, report the LC[50] or EC[50] as greater than the highest concentration and the percentage of test organisms killed or affected in each experimental treatment. If the lowest effluent concentration killed or affected more than 50 percent of the test organisms, report the LC[50] or EC[50] as less than the lowest concentration and the percentage of test organisms killed or affected in each experimental treatment. Report the method(s) used to calculate the LC[50] or EC[50], and its 95 percent confidence limits. Include a graph of the toxicity curve;
(5) Any deviations from approved methodology, along with a summary of the reason(s) for the deviation(s); and
(6) Any other relevant information; and
ix. Upon completing the toxicity test, the laboratory shall send the original or true duplicate of the results to the client. The original or true duplicate shall be signed by the laboratory manager or a designee identified under 7:18-2.11(a)1 iii.