Michigan Administrative Code
Department - State Police
Forensic Science Division
Alcohol and Drug Testing of Biological and Nonbiological Specimens
Section R. 325.2673 - Acceptable analytical methods

Universal Citation: MI Admin Code R. 325.2673

Current through Vol. 24-04, March 15, 2024

Rule 3. The following are acceptable analytical methods for determining the presence or concentration, or both, of alcohol and other drugs in blood, urine, or other various matrices or media.

(a) Liquid chromatography/mass spectrometry method using a High Performance Liquid Chromatograph/Ultra High Performance Liquid Chromatograph (HPLC/UHPLC) and mass analyzer system including, but not limited to, a quadrupole mass spectrometer, ion trap, time of flight mass spectrometer, magnetic or electromagnetic analyzer, or any hybrid combination thereof, demonstrating satisfactory accuracy, precision, sensitivity, and a suitable column for the identification or quantitation, or both, of drugs or compounds other than ethanol.

(b) Gas chromatograph method using a gas chromatograph that has satisfactory accuracy, precision, sensitivity, and a suitable column for direct injection or head-space gas chromatography for ethyl alcohol and other volatiles.

(c) Gas chromatography/mass spectrometry method using a gas chromatograph and mass spectrometer that have satisfactory accuracy, precision, sensitivity, and a suitable column for direct injection or head-space gas chromatography for identification or quantitation, or both, of drugs or compounds other than ethanol.

(d) Spectrophotometric methods as follows:

(i) Williams, Louis A. Manual of Analytical Toxicology, I. Sunshine ed., CRC Press, Cleveland, OH, 1971, pp. 309-312.

(ii) Freireich A. et al. Methodology for Analytical Toxicology, I. Sunshine ed., CRC Press, Cleveland, OH, 1975, pp. 67-69.

(e) Enzymatic and immunological methods as follows:

(i) "Stiles, et al.," Am J Clin Path., 46:608, 1966.

(ii) "Bonnichsen and Lundgren," J Acta Pharmacol Toxicol., 13:256, 1957.

(f) Analyzers as follows:

(i) Abbott Diagnostics AxSym Autoanalyzer and reagent systems.

(ii) Randox Evidence Biochip Array Analyzer and reagent systems.

(g) Analyzers or kits employing indicator-labeled immunoassays in which an indicator is attached to an antigen or antibody to demonstrate that antigen-antibody binding has occurred, thereby allowing measurement of a drug or other compound in a sample. These include the following:

(i) Enzyme immunoassay (EIA), in which an enzyme is used to label an antibody or antigen.

(ii) Enzyme-linked immunosorbent assay (ELISA), in which an enzyme-labeled antibody or antigen competes in binding with an unknown substance.

(iii) Enzyme-multiplied immunoassay technique (EMIT), which is a form of EIA used frequently for assays of drugs and hormones, as well as for viral antigens.

(iv) Fluorescence immunoassay (FIA), in which a fluorescent label is used in a competitive-binding assay.

(v) Fluorescence polarization immunoassay (FPIA), which employs fluorescent indicators that produce or detect the polarization of light.

(vi) Radioimmunoassay (RIA), which employs a radiolabeled antigen or antibody.

(vii) Chemiluminescence, in which analyte binding to an antibody is coupled to the chemical production or reduction of light output.

(viii) Any assay that uses a combination of the techniques in paragraphs (i) to (vii) of this subdivision.

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