Code of Massachusetts Regulations
310 CMR - DEPARTMENT OF ENVIRONMENTAL PROTECTION
Title 310 CMR 22.00 - Drinking Water
Section 22.26 - Ground Water Rule
Current through Register 1531, September 27, 2024
(1) General Requirements and Applicability.
(2) Sanitary Surveys for Groundwater Systems.
(3) Groundwater Source Microbial Monitoring and Analytical Methods.
If the repeat sample collected for the groundwater source is E. coli positive, the system shall comply with 310 CMR 22.26(3)(a)4.
Analytical Methods for Source Water Monitoring
Fecal Indicator 1 |
Methodology |
Method Citation |
E.coli |
Colilert®3 Colisure®3 Colilert-18® Membrane Filter Method with MI Agar E*Colite Test5 NA-MUG |
SM 9223 B2 SM 9223 B2 SM 9223 B EPA Method 16044 SM 9222 G2 |
Enterococci |
Membrane Filter Technique Membrane Filter Technique Enterolert TM 7 |
SM 9230C2 EPA Method 16006 |
Coliphage |
Two-step Enrichment Presence-absence Procedure Single Agar Layer Procedure |
EPA Method 16018 EPA Method 16029 |
Analyses must be conducted in accordance with the documents listed below. Copies may be inspected at EPA's Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW, EPA West, Room B102, Washington DC 20460 (Telephone: 202-566-2426); or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.
1 The time from sample collection to initiation of analysis may not exceed 30 hours. The groundwater system is encouraged but is not required to hold samples below 10ºC during transit.
2 Methods are described in Standard Methods for the Examination of Water and Wastewater 20th edition (1998) and copies may be obtained from the American Public Health Association, 1015 Fifteenth Street, NW, Washington, DC 20005-2605.
3 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092
4 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at http://www.epa.gov/nerlcwww/1604sp02.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, D.C. 20460.
5 A description of the E*Colite Test, Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water, January 9, 1998, is available from Charm Sciences, Inc, 659 And over St., Lawrence, MA 01843-1032 or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460.
6 EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI) EPA 821-R-02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/nerlcwww/1600sp02.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460. The holding time and temperature for groundwater samples are specified in footnote 1, rather than as specified in Section 8 of EPA Method 1600.
7 DEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters, by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881- 3884.
8 EPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at http://www.epa.gov/nerlcwww/1601ap01.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460.
9 EPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at http://www.epa.gov/nerlcwww/1601ap01.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460.
(4) Treatment Technique Requirements for Groundwater Systems.
(5) Treatment Technique Violations for Groundwater Systems.
(6) Reporting and Recordkeeping for Groundwater Systems.