Code of Maine Rules
01 - DEPARTMENT OF AGRICULTURE, CONSERVATION AND FORESTRY
001 - AGRICULTURE - GENERAL
Chapter 252 - RULES GOVERNING CERTIFICATION OF SEED POTATOES IN THE STATE OF MAINE
Appendix 001-252-I - Laboratory procedures at an authorized Nuclear Seed Origination Source involved in producing Nuclear Seed[1]

In the Laboratory & Greenhouse

1. Disease Testing Tubers

a. Sprout tubers & remove apical tip

b. Plant remainder in greenhouse

c. Disease Test2 and discard

d. Transfer tip to test tube culture medium and grow plantlet

e. Section plantlets into separate nodes,

f. Disease Test2 one node 2. Produce plantlets

3. Transplant plantlets to greenhouse.

4. Harden plants off and Disease Test2

5. Grow to produce minitubers

Planted in soil (First Year) (See Section 2. B.)

1. Plant minitubers in field (Disease Test2)

2. Transplant plantlets into field (Disease Test2)

1 Other methods of Pre-nuclear Seed production are acceptable. (See 2.C.2.)

2 Laboratory test means testing for the Bacterial Ring Rot, Blackleg, PLRV, PVA, PVS, PVX, PVY, PVY-N, Potato Mop Top Virus, and PSTV pathogens.