Louisiana Administrative Code
Title 7 - AGRICULTURE AND ANIMALS
Part XXXI - Milk, Milk Products and Substitutions
Chapter 1 - Milk Testing
Subchapter B - Approved Methods of Testing
Section XXXI-115 - Procedure; Testing Skim Milk for Butterfat Content

A. Approved Methods. The official methods of analysis shall be the Majonnier, Babcock and any other method approved by the commissioner of agriculture.

B. Babcock Procedure (Modified). Measure nine milliliters of skim milk into the skim milk test bottle. Add two milliliters of N-butyl alcohol and mix for three minutes in mechanical shaker. Add 7 to 9 milliliters of sulphuric acid (sp. gr. 1.82 to 1.83) to contents and mix for three to five minutes in shaker. Centrifuge for six minutes and add 140-150° F water to within 1/4 inch of graduated neck of the test bottle. Centrifuge for two minutes and add 140 to 150 water into the upper section of the graduated neck of the test bottle. Centrifuge for two minutes. Remove tests and place in 138-140° F tempering bath for five minutes. Measure the column and double the test result. Post test result immediately. Drain and clean glassware and equipment immediately upon termination of testing procedure.