Indiana Administrative Code
Title 327 - WATER POLLUTION CONTROL DIVISION
Article 8 - PUBLIC WATER SUPPLY
Rule 2 - Drinking Water Standards
Section 2-8.7 - Analytical and monitoring requirements; fecal coliform, total coliform, turbidity, disinfection for public water systems

Universal Citation: 327 IN Admin Code 2-8.7

Current through September 18, 2024

Authority: IC 13-13-5; IC 13-14-8-7; IC 13-14-9; IC 13-18-3; IC 13-18-16

Affected: IC 13-11-2; IC 13-14-8; IC 13-18-1; IC 13-18-2

Sec. 8.7.

Only the analytical methods and procedures specified in this section, or otherwise approved by EPA, may be used to demonstrate compliance by public water systems with sections 8.5 and 8.6 of this rule. Measurements for residual disinfectant concentrations must be conducted using methods specified in this rule. Analysis of pH and temperature must be conducted in accordance with one (1) of the methods listed at 40 CFR 141.23(k)(1). Analysis of total coliforms, fecal coliforms, heterotrophic bacteria, and turbidity must be conducted in accordance with one (1) of the following analytical methods or one (1) of the alternate methods listed in Appendix A to Subpart C of 40 CFR 141, and by using analytical test procedures contained in Technical Notes on Drinking Water Methods, EPA-600/R-94-173, October 1994. This document is available from the National Service Center for Environmental Publications (NSCEP), P.O. Box 42419, Cincinnati, OH 45242-0419 or http://www.epa.gov/nscep/. Measurements for total coliforms, fecal coliforms, and HPC must be conducted by a laboratory certified by the commissioner or EPA under 40 CFR 141.28 *. Until laboratory certification criteria are developed for the analysis of fecal coliforms and HPC, any laboratory certified for total coliforms analysis by the commissioner or EPA is deemed certified for fecal coliforms and HPC analysis. The following procedures shall be conducted in accordance with the publications listed as follows:

(1) Total coliform1 as set forth in the following:

(A) Total coliform fermentation technique2, 3, 4, Method 9221A*, and B*.

(B) Total coliform membrane filter technique7 6, Method 9222A*, B*, and C*.

(C) ONPG-MUG test membrane5, Method 9223*.

(D) Presence-Absence (P-A) coliform test4, 7, Method 9221D*.

(E) Colisure test8*.

(F) E*Colite test*.

(G) m-ColiBlue24 test*.

(H) Readycult Coliforms 100 Presence/Absence test*.

(I) Membrane Filter Technique using Chromocult Coliform Agar*.

(J) Colitag test*.

(2) Fecal coliforms1 as set forth in:

(A) fecal coliform procedure 9, Method 9221E*; or

(B) fecal coliform filter procedure, Method 9222D.

(3) Heterotrophic bacteria1, Method 9215B*, pour plate method.

(4) Turbidity as set forth in:

(A) nephelometric method, Method 2130B* or Method 180.1*; or

(B) Great Lakes Instruments method, Method 2*.

(5) Residual disinfectant concentrations for free chlorine and combined chlorine (chloramines) as set forth in the following methods or with the alternative methods listed in Appendix A to Subpart C of 40 CFR 141. If approved by the commissioner, residual disinfectant concentrations for free chlorine and combined chlorine also may be measured using DPD colorimetric test kits. In addition, the commissioner may approve the use of the ITS free chlorine test strip for the determination of free chlorine. Use of the test strips is described in Method D99-003, "Free Chlorine Species (HOCl- and Ocl-) by Test Strip", Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730. Free and total chlorine residuals may be measured continuously by adapting a specific chlorine residual method for use with a continuous monitoring instrument provided the chemistry, accuracy, and precision remain the same. Instruments used for continuous monitoring must be calibrated with a grab sample measurement at least every five (5) days, or with a protocol approved by the commissioner:

(A) Method 4500-Cl D*, amperometric titration method.

(B) Method 4500-Cl F*, DPD ferrous titrimetric method.

(C) Method 4500-Cl G*, DPD colorimetric method.

(D) Method 4500-Cl H*, syringaldazine (FACTS).

(E) DPD colorimetric test kits, if approved by the commissioner.

(F) Free chlorine residuals may be measured continuously by adapting a specified chlorine residual method for use with a continuous monitoring instrument, provided the chemistry, accuracy, and precision remain the same. Instruments used for continuous monitoring must be calibrated with a grab sample measurement at least every five (5) days, or with a protocol approved by the commissioner.

(6) Residual disinfectant concentrations for ozone by the indigo method, Method 4500-O3 B*.

(7) Residual disinfectant concentrations for chlorine dioxide must be measured by Method 4500-ClO2 C, amperometric method, Method 4500-ClO2 E*, amperometric method, or Method 4500-ClO2 D*, DPD method.

(8) Residual disinfectant concentrations for total chlorine by the following methods:

(A) Method 4500-Cl D*, amperometric titration.

(B) Method 4500-Cl E*, amperometric titration (low level measurement).

(C) Method 4500-Cl F*, DPD ferrous titrimetric.

(D) Method 4500-Cl I, iodometric electrode.

(E) Method 4500-Cl G*, DPD colorimetric.

(F) Total chlorine residuals may be measured continuously by adapting a specified chlorine residual method for use with a continuous monitoring instrument, provided the chemistry, accuracy, and precision remain the same. Instruments used for continuous monitoring must be calibrated with a grab sample measurement at least every five (5) days, or with a protocol approved by the commissioner.

*The following methods are incorporated by reference:

(1) Methods 2130B, 4500-Cl D, 4500-Cl E, 4500-Cl F, 4500-Cl G, 4500-Cl H, 4500-CL-I, 4500-ClO2 C, 4500-ClO2 D, 4500-ClO2 E, 9215B, 9221A, 9221B, 9221D, 9221E, 9222A, 9222B, 9222C, 9222D, and 9223 may be found in "18th Edition of Standard Methods for the Examination of Water and Wastewater", "19th Edition of Standard Methods for the Examination of Water and Wastewater", and "20th Edition of Standard Methods for the Examination of Water and Wastewater", 1992, 1995, and 1998 available from the American Public Health Association, 1015 Fifteenth Street, Washington, D.C. 20005. The cited methods published in any of these three (3) editions may be used.

(2) Method 4500-O3 B may be found in "18th Edition of Standard Methods for the Examination of Water and Wastewater" and "19th Edition of Standard Methods for the Examination of Water and Wastewater", 1992 and 1995, available from the American Public Health Association, 1014 Fifteenth Street, Washington, D.C. 20005. Either edition may be used.

(3) A description of the Colisure Test, February 28, 1994, may be obtained from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092.

(4) A description of the E*Colite test, "Presence/Absence for Coliforms and E. coli in Water", December 21, 1997, is available from Charm Sciences, Inc., 36 Franklin Street, Malden, Massachusetts 02148-4120.

(5) A description of the m-ColiBlue24 test, August 17, 1999, is available from the Hach Company, 100 Dayton Avenue, Ames, Iowa 50010.

(6) The ReadyCult Coliforms 100 Presence/Absence Test is described in the document "ReadyCult Coliforms 100 Presence/Absence Test for Indication of Coliform Bacteria and Escherichia coli in Finished Waters", November 2000, Version 1.0, available from EM Science, an affiliate of Merck KggA of Darmstadt, Germany, 480 South Democrat Road, Gibbstown, New Jersey 08027-0342.

(7) Membrane Filter Technique using Chromocult Coliform Agar is described in the document "Chromocult Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters", November 2000, Version 1.0, available from EM Science, an affiliate of Merck KggA of Darmstadt, Germany, 480 South Democrat Road, Gibbstown, New Jersey 08027-0342.

(8) Colitag product for the determination of presence/absence of total coliforms and E. coli is described in "Colitag Product as a Test for Detection and Identification of Coliforms and E. coli Bacteria in Drinking Water and Source Water as Required in National Primary Drinking Water Regulations", August 2001, available from CPI International, Inc., 5580 Skylane Drive, Santa Rosa, California 95403. The telephone number is (800) 878-7654.

(9) Method 180.1 may be found in "Methods for the Determination of Inorganic Substances in Environmental Samples", EPA-600/R-93-100, August 1993, available from NTIS, PB94-121811, U.S. Department of Commerce, 5285 Port Royal Road, Springfield, Virginia 22161.

(10) The Great Lakes Instrument (GLI) Method 2 may be found in "Turbidity", November 2, 1992, Great Lakes Instruments, Inc., 8855 North 55th Street, Milwaukee, Wisconsin 53223.

(11) 40 CFR 141.28 may be obtained from the Superintendent of Documents, Government Printing Office, Washington, D.C. 20402.

These methods are available for copying at the Indiana Department of Environmental Management, Office of Water Quality, 100 North Senate Avenue, Room N1255, Indianapolis, Indiana 46204.

1The time from sample collection to initiation of analysis may not exceed thirty (30) hours. Systems must hold samples below ten (10) degrees Celsius during transit.

2Lactose broth, as commercially available, may be used instead of lauryl tryptose broth if the system conducts at least twenty-five (25) parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliforms using lactose broth, is less than ten percent (10%).

3Media should cover inverted tubes at least one-half (1/2) to two-thirds (2/3) after the sample is added.

4No requirement exists to run the completed phase on ten percent (10%) of all total coliform-positive confirmed tubes.

5The ONPG-MUG test is also known as the Autoanalysis Colilert System.

6MI Agar may also be used.

7Six (6) times formulation strength may be used if the medium is filter-sterilized rather than autoclaved.

8The Colisure test may be read after an incubation time of twenty-four (24) hours.

9A-1 broth may be held up to three (3) months in a tightly closed screwcap tube at four (4) degrees Celsius.

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