Hawaii Administrative Rules
Title 11 - DEPARTMENT OF HEALTH
Subtitle 1 - GENERAL DEPARTMENTAL PROVISIONS
Chapter 20 - RULES RELATING TO PUBLIC WATER SYSTEMS
Appendix K - ANALYTICAL METHODS FOR CONDUCTING TOTAL COLIFORM AND E. COLI ANALYSES (HAR Section 11-20-9.1 (b))

Universal Citation: HI Admin Rules K

Current through February, 2024

Organism	Methodology Category	Method1	Citation1
Total Coliforms
	Lactose Fermentation Methods	Standard Total Coliform Fermentation Technique	Standard Methods 9221 B.1, B.2 (20th ed.; 21st ed.) 2, 3 Standard Methods Online 9221 B.1, B.2-99 2,3
	Lactose Fermentation Methods	Presence-Absence (P-A) Coliform Test	Standard Methods 9221 D.1, D.2 (20th ed.; 21st ed.) 2, 7 Standard Methods Online 9221 D.1, D.2-99 2, 7
	Membrane Filtration Methods	Standard Total Coliform Membrane Filter Procedure	Standard Methods 9222 B, C (20th ed.; 21st ed.) 2, 7 Standard Methods Online 9222 B-97 2, 4, 9222 C-97 2, 4
		Membrane Filtration using MI medium	EPA Method 1604 2
		m-ColiBlue24® Test 2, 4
		Chroraocult 2, 4
	Enzyme Substrate Methods	Colilert®	Standard Methods 9223 B (20th ed.; 21st ed.) 2, 5 Standard Methods Online 9223 B-97 2, 5
		Colisure®	Standard Methods 9223 B (20th ed.; 21st ed.) 2, 5, 6 Standard Methods Online 9223 B-97 2, 5, 6
		E*Colite® Test 2
		Readycult® Test 2
		modified Colitag® Test 2
	Escherichia coli
		Escherichia coli Procedure (following Lactose Fermentation Methods)	EC-MUG medium	Standard Methods 9221 F.1 (20th ed.; 21st ed.) 2
		Escherichia coli Partition Method	EC broth with MUG (EC-MUG)	Standard Methods 9222 G.1c(2)(20th ed.; 21st ed.) 2, 8
		NA-MUG medium	Standard Methods 9222 G.1c(1) (20th ed.; 21st ed.) 2
Membrane Filtration Methods		Membrane Filtration using MI medium	EPA Method 1604 2
Membrane Filtration Methods		m-ColiBlue24® Test 2, 4
		Chromocult 2, 4

		Enzyme Substrate Methods	Colilert®	Standard Methods 9223 B (20th ed.; 21st ed.) 2, 5 Standard Methods Online 9223 B-97 2, 5, 6
	Colisure®		Standard Methods 9223 B (20th ed.; 21st ed.) 2, 5, 6
			Standard Methods Online 9223 B-97 2, 5, 6
	E*Colite® Test 2
	Readycult® Test 2
	modified Colitag® Test 2

(a) Footnotes to Appendix K

1 The procedures must be done in accordance with the documents listed in subsection (b) of this Appendix, For Standard Methods, either editions, 20th (1998) or 21st (2005), may be used. For the Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions that may be used. For vendor methods, the date of the method listed in subsection (b) of this Appendix is the date/version of the approved method. The methods listed are the only versions that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods, as product package inserts may not be the same as the approved versions of the methods.

2 Incorporated by reference. See subsection (b) of this Appendix.

3 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broths if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and false-negative rate for total coliforms, using lactose broth, is less than 10 percent.

4 All filtration series must begin with membrane filtration equipment that has been sterilized by autoclaving. Exposure of filtration equipment to UV light is not adequate to ensure sterilization. Subsequent to the initial autoclaving/ exposure of the filtration equipment to UV light may be used to sanitize the funnels between filtrations within a filtration series. Alternatively, membrane filtration equipment that is pre-sterilized by the manufacturer (i.e., disposable funnel units) may be used.

5 Multiple-tube and multi-well enumerative formats for this method are approved for use in presence-absence determination under this regulation.

6 Colisure® results may be read after an incubation time of 24 hours.

7 A multiple tube enumerative format, as described in Standard Methods for the EKamination of Water and Wastewater 9221, is approved for this method for use in presence-absence determination under this regulation.

8 The following changes must be made to the EC broth with MUG (EC-MUG) formulation: Potassium dihydrogen phosphate, KH2PO4, must be 1.5g, and 4-methylumbelliferyl-Beta-D-glucuronide must be 0.05 g.

(b) Incorporation by reference. The standards required in § 11-20-9.1 are incorporated by reference into this section with the approval of the Director of the Federal Register under 5 U.S.C, 552(a) and 1 CFR part 51. To enforce any edition other than that specified in this section, EPA must publish notice of change in the Federal Register and the material must be available to the public. All approved material is available for inspection either electronically at www.regulations.gov, in hard copy at the Water Docket, or from the sources indicated below. The Docket ID is EPA-HQ-OW-2008-0878. Hard copies of these documents may be viewed at the Water Docket in the EPA Docket Center, (EPA/DC) EPA West, Room 3334, 1301 Constitution Ave., NW, Washington, DC, The EPA Docket Center Public Reading Room is open from 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding legal holidays. The telephone number for the Public Reading Room is 1-202-566-1744, and the telephone number for the Water Docket is 1-202-566-2426. Copyrighted materials are only available for viewing in hard copy. These documents are also available for inspection at the National Archives and Records Administration (NARA), For information on the availability of this material at NARA, call 1-202-741-6030 or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_ locations.html.

(1) American Public Health Association, 800 I Street, NW, Washington, DC 20001

(i) "Standard Methods for the Examination of Water and Wastewater," 20th edition (1998):

(A) Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," B.1, B.2, "Standard Total Coliform Fermentation Technique."

(B) Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," D.1, D.2, "Presence-Absence (P-A) Coliform Test."

(C) Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," B, "Standard Total Coliform Membrane Filter Procedure."

(D) Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," C, "Delayed-Incubation Total Coliform Procedure."

(E) Standard Methods 9223, "Enzyme Substrate Coliform Test," B, "Enzyme Substrate Test," Colilert® and Colisure®.

(F) Standard Methods 9221, "Multiple Tube Fermentation Technique for Members of the Coliform Group," F.1, "Escherichia coli Procedure: EC-MUG medium."

(G) Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.1.c(2), "Escherichia coli Partition Method: EC broth with MUG (EC-MUG)."

(H) Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.1.c(1), "Escherichia coli Partition Method: NA-MUG medium."

(ii) "Standard Methods for the Examination of Water and Wastewater," 21st edition (2005):