(2)
Procedures in selected herds.
(a) Infected
herds.
1. In infected herds, the individual
herd plan will include the use of whole herd vaccination in addition to other
recognized procedures for elimination of the disease. All test eligible animals
must have an official brucellosis test and all reactors must be removed for
immediate slaughter. Negative female animals must be vaccinated with a reduced
dose of vaccine within 10 days of the test. All eligible calves shall also be
vaccinated and identified.
2.
Overage vaccinates shall be identified by an official numbered metal eartag in
the right ear and, in the left ear, may be further identified by a large
numbered unique tag correlated on test records with the official eartag. A
permanent legible individual animal registration or individual animal
registration brand may be used in lieu of the official numbered metal eartag.
Additionally, each animal shall be identified at the time of vaccination with
an open-ended "V" hot-iron brand at least two inches in height and two inches
in width on the right jaw, or by an official AV (adult vaccination) tattoo in
the right ear preceded by the quarter of the year and followed by the last
digit of the year in which the vaccination was done, or both.
3. The herd must be maintained in quarantine,
and a first retest is recommended at two months or as early as possible
following vaccination to facilitate elimination of field strain infection.
Complete herd testing shall be resumed not more than six months following the
initial whole herd vaccination, and shall be conducted at such intervals as are
established by the designated epidemiologist. Reactor animals must be removed
for slaughter following each herd test.
4. Brucellosis infected adult vaccinated beef
herds shall be released from quarantine on two negative tests, the first not
less than 30 days following removal of all reactors and the second not less
than 180 days following removal of the last reactor, as is currently provided
in subsection 5C-6.0019(3),
F.A.C., for release of quarantine in infected nonvaccinated herds. Such herds
shall be considered negative if all animals in the herd show titers of less
than +1 at 1:40 on the CF test or less than +25 on the rivanol test. An
additional herd test is required not less than six months after release of a
vaccinated herd from quarantine. A post-sale retest on animals moving from
previously quarantined "AV" herds before the six-months retest shall be
conducted between 120 and 180 days. However, animals may be tested as early as
45 days after movement if, at the time of test, at least six months have
expired since the quarantine release of the herd of origin.
5. In all herd tests, a presumptive test
shall be performed on each test-eligible animal. The card, and CF and/or
rivanol tests shall be performed on all presumptive test positive animals.
Animals positive to any of these tests, that are not identifiable as official
vaccinates, shall be classified as reactors.
6. Official vaccinates positive on the
standard card test or having a titer between +2 at 1:10 to +4 at 1:20 on the CF
test in herds eligible for release from quarantine shall be classified as
suspects and be quarantined individually (not the whole herd) until they are
card negative and less than +2 at 1:10 on the CF test.
7. Dairy Herds. All of the foregoing
procedures in Rule 5C-6.0021, F.A.C., shall apply
to "AV" dairy herds. In addition, dairy herds shall either be negative to the
last milk ring test or all individual lactating "AV" dairy animals shall have
less than a 1:16 titer by the milk ring test on individual samples of all
quarters prior to release from quarantine. Any "AV" dairy herd released from
quarantine which continues to be suspicious on milk ring test surveillance
shall be evaluated by the designated epidemiologist and monitored with further
testing as necessary to ensure that there is no evidence of field strain
brucella infection in the herd.
(b) Herds which are not Certified
Brucellosis-Free.
1. Persons owning herds at
high risk of becoming infected with brucellosis which are not Certified
Brucellosis-Free, may also request whole herd vaccination. The individual herd
plan will then include the use of whole herd vaccination in addition to other
recognized procedures for elimination of the disease in accordance with
paragraph 5C-6.002(2)(a),
F.A.C. above. All eligible calves shall be calfhood vaccinated and identified.
In addition to an official eartag, all animals so adult vaccinated shall also
be identified by an open-ended "V" hot-iron brand on the right jaw, or high on
the hip near the tailhead or an official "AV" (adult vaccination) tattoo in the
right ear or both. Following vaccination, the adult vaccinated animals will be
placed under quarantine. Included in the individual herd plan shall be
provisions for the herd to be tested within 30 to 120 days following
vaccination, and at intervals as agreed to in the individual herd plan, until a
negative test conducted at least 90 days later than the first negative test 30
days or longer after the last reactor, if any, is removed qualifies the herd to
be released from quarantine.
2.
Dairy Herds. All of the foregoing shall apply to "AV" dairy herds. In addition,
dairy herds shall either be negative to the last milk ring test or all
individual lactating "AV" dairy animals shall have less than a 1:16 titer by
the milk ring test on individual samples of all quarters prior to release from
quarantine. Any "AV" dairy herd released from quarantine which continues to be
suspicious on milk ring test surveillance shall be evaluated by the designated
epidemiologist and monitored with further testing as necessary to ensure that
there is no evidence of field strain brucella infection in the herd.
3. If infection is disclosed on the
pre-vaccinal or subsequent herd tests prior to quarantine release, an
additional herd test is required not less than six months after release of a
vaccinated herd from quarantine. A post-sale retest on the animals moving from
previously quarantined "AV" herds before the six months retest is required
between 120 and 180 days. Animals may be tested as early as 45 days after
movement if, at the time of test, at least six months have expired since the
quarantine release of the herd of origin.
(c) Certified Brucellosis-Free Herds. Under
this plan, owners of Certified Brucellosis-Free Herds in an area of high risk
of becoming infected with brucellosis may request whole herd vaccination. The
purpose is to immediately establish a high level of resistance to the present
or expected exposure from field strain Brucella abortus.
1. In herds of known status; i.e., Certified
Brucellosis-Free Herds, the individual herd plan will include the following
whole herd vaccination procedures in addition to other recognized procedures
for prevention or control of the disease:
a. A
blood sample shall be obtained from all test-eligible animals for serologic
testing. If all tests are negative, the herd will be considered a Certified
Brucellosis-Free Herd at high risk, but if any animals are reactors, the herd
will be declared an infected herd and will follow the same rules for known
infected herds as established in Rule
5C-6.0021, F.A.C.
b. In Certified Brucellosis-Free Herds at
high risk, all eligible calves shall be calfhood vaccinated and identified.
Within 10 days after negative serologic testing, all other female animals shall
be vaccinated with a reduced dose of strain 19 vaccine and identified with an
official "AV" (adult vaccination) tattoo in the right ear as well as the
permanent individual official metal eartag. Following vaccination, the adult
vaccinated animals will be placed under quarantine. Included in the individual
herd plan shall be provisions for the herd to be tested within 30 to 180 days
following vaccination. However, upon written request by the owner, the Area
Veterinarian in Charge or Chief State Animal Health Official may extend the
time of the first post-vaccination herd test to coordinate it with the
recertification requirements. The herd must be retested at not less than 30-day
intervals thereafter, until a negative test conducted at least 150 days later
than the first negative test 30 days or longer after the last reactor, if any,
is removed qualifies to release the quarantine. Certified Brucellosis-Free Herd
status is suspended while the adult vaccinated animals remain under quarantine
and is reinstated upon release from quarantine provided field strain
Brucella abortus infection has not been diagnosed in the herd.
During such period of quarantine, standard card test negative adult vaccinated
animals in these Certified Brucellosis-Free Herds may move without
restriction.
2. Dairy
Herds. All of the foregoing shall apply to "AV" dairy herds. In addition, dairy
herds shall either be negative to the last milk ring test or all individual
lactating "AV" dairy animals shall have less than a 1:16 titer by the milk ring
test on individual samples of all quarters prior to release from quarantine.
Any "AV" dairy herd released from quarantine which continues to be suspicious
on milk ring test surveillance shall be evaluated by the designated
epidemiologist and monitored with further testing as necessary to ensure that
there is no evidence of field strain brucella infection in the herd.
3. If infection is disclosed on the
pre-vaccinal or subsequent herd tests prior to quarantine release, an
additional herd test is required not less than six months after release of a
vaccinated herd from quarantine. A post-sale retest on the animals moving from
previously quarantined "AV" herds before the six months retest is required
between 120 and 180 days. Animals may be tested as early as 45 days after
movement if, at the time of test, at least six months have expired since the
quarantine release of the herd of origin.