Current through Reg. 50, No. 187; September 24, 2024
(1) The following methods of testing shall be
considered as screening tests:
(a) Milk Ring
Test (BRT). This test is conducted by a state-federal laboratory on composite
samples of milk collected at dairy farms. A follow-up individual serological
test shall be conducted on all cattle represented in a composite sample which
reacts to the test. The milk ring test may be used in conjunction with a
negative herd blood test to establish a Certified Brucellosis-Free
Herd.
(b) Buffered Acidified Plate
Antigen (BAPA) presumptive test. This test will be used by a state-federal
laboratory to classify cattle and bison negative on surveillance samples
collected at slaughter or at livestock markets, on routine samples collected on
farms, and on tests of suspicious and infected herds. Positive samples will be
given supplemental testing to aid in classification.
(c) Standard Card Test. This test will be
used by a state-federal laboratory to classify cattle and bison negative on
surveillance samples collected at slaughter or at livestock markets, on routine
samples collected on farms, and on tests of suspicious and infected herds.
Positive samples will be given supplemental testing to aid in
classification.
(d) Particle
Concentration Fluorescence Immunoassay (PCFIA). All samples with results within
the suspect range will be given supplemental testing to aid in
classification.
(2) The
following methods of testing when conducted in a state-federal laboratory shall
be considered as official tests for the classification of cattle and bison with
regard to brucellosis status:
(a) Standard
tube agglutination test. This test may classify cattle and bison as negative,
suspect, or reactor.
(b) Standard
plate agglutination test. This test may classify cattle and bison as negative,
suspect, or reactor.
(c) Standard
card test. The standard card test is to be used as an official test to classify
cattle and bison as reactors only when conditions and time are such that no
other test is available, or upon request of the owner because of time or
situation constraints, or in livestock markets where the standard card test has
been designated as the official test. All samples tested at other than a
state-federal laboratory shall be promptly submitted and confirmed at a
state-federal laboratory.
(d)
Rivanol test. This test may classify cattle and bison as negative, suspect, or
reactor.
(e) Complement-fixation
test. This test may classify cattle and bison as negative, suspect, or
reactor.
(f) Semen plasma test.
This test may classify bulls used for artificial insemination as negative or
reactor when used in conjunction with an official serological test.
(g) Particle Concentration Fluorescence
Immunoassay (PCFIA). This test may classify cattle and bison as negative,
suspect, or reactor. All samples with results within the suspect range will be
given supplemental testing to aid in classification.
(3) The following diagnostic procedures shall
be considered as supplemental tests and, when requested by the designated
epidemiologist, shall be used to aid in determining the presence or absence of
blood components associated with field strains of Brucella abortus, Brucella
abortus strain 19, nonspecific agglutinins, etc.:
(a) Mercaptoethanol (ME)
(b) Coombs
(c) Heat inactivation (HI)
(d) Fluorescent Antibody (FA)
(e) Enzyme Labeled Antibody (ELA)
(f) Biological culture
(g) Acid Plate Antigen (APA)
(h) Concentration Immunoassay Technology
(CITE)
(4) Standard tube
agglutination test and standard plate agglutination test.
(a) Non-vaccinates. Blood titer showing
complete reaction in the 1:100 dilution or higher shall be classed as a
reactor. Titer of not more than incomplete in the 1:100 dilution shall be
classed as a suspect. Titer less than incomplete in the 1:50 dilution shall be
considered negative.
(b) Official
vaccinates. Blood titer showing complete reaction in the 1:200 dilution or
higher shall be classed as a reactor. Titer showing any reaction in the 1:100
dilution or incomplete in the 1:200 dilution shall be classed as a suspect.
Titer less than incomplete in the 1:100 dilution shall be considered
negative.
(5) Standard
card test. Blood showing a positive reaction to the standard card test will be
classed as a reactor when only the standard card test has been conducted.
Cattle and bison positive to the standard card test which are serologically
negative to all other official tests which are conducted are classified as
suspects.
(6) Rivanol test.
(a) The rivanol test is an official test when
conducted in a state-federal laboratory. Complete agglutination at dilutions of
1:25 or more indicates a reactor in nonvaccinated animals and in vaccinated
animals when the complement-fixation test is not conducted. Less than complete
agglutination at the 1:25 dilution shall be considered negative.
(b) Officially vaccinated animals with
agglutination complete in the 1:50 dilution or less may be classified as
suspects when those interpretations are supported by complement-fixation test
results of suspect or negative using the presently accepted complement-fixation
test classification scheme for vaccinated animals.
(c) Incomplete or complete agglutination in
the 1:100 dilution or in higher dilutions indicates a reactor in vaccinated
animals.
(d) Within five months
postvaccination of adult animals, a less than complete agglutination at the
1:50 dilution is considered negative.
(7) Complement-fixation test.
(a) Official vaccinates, including adult
vaccinated animals beginning two months postvaccination. Animals with titers of
25 percent fixation (1 plus) in a dilution of 1:40 or higher will be classified
as reactors. Animals having less than 25 percent fixation (1 plus) in 1:40
dilution, and 50 percent (2 plus) or more in a dilution of 1:10 are classified
as suspects. A reaction of less than 50 percent fixation (2 plus) in the 1:10
dilution is considered negative.
(b) Other cattle and bison. A 50 percent
fixation (2 plus) in a dilution of 1:20 or higher will be classed as a reactor;
fixation of less than 50 percent (2 plus) in a dilution of 1:20 and 50 percent
fixation (2 plus) or more in a dilution of 1:10 will be classed as a suspect;
less than 50 percent fixation (2 plus) in a dilution of 1:10 will be classed as
negative.
(8) Particle
Concentration Fluorescence Immunoassay (PCFIA). This test may be used as a
presumptive or diagnostic test with the following diagnostic criteria:
(a) Negative - values greater than
0.70.
(b) Suspect - values less
than or equal to 0.70 and greater than 0.25.
(c) Reactor - values less than or equal to
0.25.
(d) Values may be interpreted
differently if done by a veterinarian, qualified by education, training, and
experience and certified by the United States Department of Agriculture with
the concurrence of the state and termed designated
epidemiologist.
(9) Semen
plasma test. This test in bulls used for artificial insemination must be used
in conjunction with an official serological test, and classification will be
based on the maximum titer of either test.
(10) Any animal found to be infected through
isolation of field strain Brucella abortus must be classed as a
reactor.
(11) Evaluation of titer
responses for all cattle and bison shall be the responsibility of a designated
epidemiologist, who takes into consideration animal and herd history and other
epidemiologic considerations.
(12)
Interpretation of market tests. Blood samples collected at slaughter are
considered negative if no evidence of agglutination is disclosed on the
buffered acidified plate antigen test. Samples showing agglutination are
classified negative if the confirmatory standard card test is
negative.
Rulemaking Authority 585.002(4), 585.08(2) FS. Law
Implemented 585.08(1), (3), 585.11(1), (2), 585.155(1), (2), (3), (4)
FS.
New 6-1-92, Amended
7-5-95.
