Government-Owned Inventions; Availability for Licensing, 52934-52936 [2013-20888]
Download as PDF
52934
Federal Register / Vol. 78, No. 166 / Tuesday, August 27, 2013 / Notices
FormsSubmissionRequirements/
ElectronicSubmissions/ucm364432.htm.
SUPPLEMENTARY INFORMATION:
I. Funding Opportunity Description
tkelley on DSK3SPTVN1PROD with NOTICES
RFA–FD–13–039
93.103
A. Background
CDER receives an enormous and
growing amount of data in a variety of
regulatory submissions from a multitude
of sources and in a variety of formats.
This wealth of data holds great potential
to advance CDER’s regulatory and
scientific work, but the present lack of
standardized data creates significant
challenges to realizing that potential.
The volume and complexity of drugrelated information submitted to CDER
for regulatory review is creating
significant challenges to the Center’s
ability to efficiently and effectively
perform its critical public health
mission.
The lack of standardized data affects
CDER’s review processes by curtailing a
reviewer’s ability to perform integral
tasks such as rapid acquisition, analysis,
storage, and reporting of regulatory data.
Improved data quality, accessibility, and
predictability will give reviewers more
time to carry out complex analyses, ask
in-depth questions, and address lateemerging issues. Standardized data will
allow reviewers to increase review
consistency and perform evaluations
across the drug lifecycle. This will
enhance the Center’s performance
across key drug regulatory functions and
ongoing business operations, including
premarket review, post-market safety,
oversight of drug quality, and oversight
of drug promotion.
Standardized data elements that are
common to all clinical trials, such as age
and gender, have been established
through Clinical Data Interchange
Standards Consortium standards.
However, data elements that are unique
for a particular disease or therapeutic
area still need to be developed so that
the data are consistent and consistently
understood for efficacy analysis, and
that data from multiple trials can be
more easily grouped for reporting and
meta-analysis.
In short, establishing common
standards for data reporting will provide
new opportunities to transform the
massive amount of data from drug
studies on specific diseases into useful
information to potentially speed the
delivery of new therapies to patients.
B. Research Objectives
The CFAST Initiative aims to
accelerate clinical research and medical
product development by establishing
VerDate Mar<15>2010
15:54 Aug 26, 2013
Jkt 229001
and maintaining data standards, tools,
and methods for conducting research in
therapeutic areas that are important to
public health. It is established as a
public-private partnership (PPP)
involving multiple stakeholders. The
Grantee funded through this
announcement would be expected to
accomplish activities such as, but not
limited to:
• Maintenance of the scientific and
administrative infrastructure of the PPP
to support a series of projects under the
CFAST Initiative.
• Coordination and management of
therapeutic area standards development
projects with key experts in the specific
therapeutic areas, including
stakeholders from industry, professional
organizations, academia, and
Government agencies.
• Identification and engagement with
key experts in the therapeutic areas,
including stakeholders from industry,
professional organizations, academia,
and Government agencies.
• Development of therapeutic area
data standards, initially proposed for
diabetes, QT studies, lipid lowering/
altering drugs, and hepatitis C.
Additional or different areas can be
considered as well.
• Identification and implementation
of continuous quality improvements
with respect to the data standards
development process and product(s) to
facilitate timely and sustainable
standards.
C. Eligibility Information
The following organization is eligible
to apply: The Critical Path Institute (CPath).
Over the past 7 years, C-Path has
become an international leader in
forming and leading/managing
collaborations globally. They currently
lead 7 very active scientific consortia
across multiple disease areas. C-Path
consortia include more than 1,000
scientists from Government, academia,
patient advocacy organizations, and 41
major pharmaceutical companies. CPath has a proven process, capability,
and institutional knowledge critical to
successfully leading scientific consortia
and rapid therapeutic area standards
development projects through an open,
transparent process as identified by the
Prescription Drug User Fee Act V.
II. Award Information/Funds Available
A. Award Amount
Frm 00039
Fmt 4703
III. Paper Application, Registration,
and Submission Information
To submit a paper application in
response to this FOA, applicants should
first review the full announcement
located at https://www.fda.gov/Drugs/
DevelopmentApprovalProcess/
FormsSubmissionRequirements/
ElectronicSubmissions/ucm364432.htm.
(FDA has verified the Web site
addresses throughout this document,
but FDA is not responsible for any
subsequent changes to the Web sites
after this document publishes in the
Federal Register.) Persons interested in
applying for a grant may obtain an
application at https://www.fda.gov/
Drugs/DevelopmentApprovalProcess/
FormsSubmissionRequirements/
ElectronicSubmissions/ucm364432.htm.
For all the paper application
submissions, the following steps are
required:
• Step 1: Obtain a Dun and Bradstreet
(DUNS) Number
• Step 2: Register With System for
Award Management (SAM)
• Step 3: Register With Electronic
Research Administration (eRA)
Commons
Steps 1 and 2, in detail, can be found
at https://www07.grants.gov/applicants/
organization_registration.jsp. Step 3, in
detail, can be found at https://
commons.era.nih.gov/commons/
registration/registrationInstructions.jsp.
After you have followed these steps,
submit paper applications to: Kimberly
Pendleton-Chew, 5630 Fishers Lane,
Rm. 2031, Rockville, MD 20857, 301–
827–9363, email: Kimberly.Pendleton@
fda.hhs.gov.
Dated: August 21, 2013.
Leslie Kux,
Assistant Commissioner for Policy.
[FR Doc. 2013–20823 Filed 8–26–13; 8:45 am]
BILLING CODE 4160–01–P
DEPARTMENT OF HEALTH AND
HUMAN SERVICES
National Institutes of Health
Government-Owned Inventions;
Availability for Licensing
AGENCY:
National Institutes of Health,
HHS.
ACTION:
Notice.
The inventions listed below
are owned by an agency of the U.S.
Government and are available for
SUMMARY:
Total amount of funding available is
$2,000,000. Anticipate one award.
PO 00000
B. Length of Support
Scope of the proposed project should
determine the project period. The
maximum period is 3 years.
Sfmt 4703
E:\FR\FM\27AUN1.SGM
27AUN1
Federal Register / Vol. 78, No. 166 / Tuesday, August 27, 2013 / Notices
tkelley on DSK3SPTVN1PROD with NOTICES
licensing in the U.S. in accordance with
35 U.S.C. 209 and 37 CFR Part 404 to
achieve expeditious commercialization
of results of federally-funded research
and development. Foreign patent
applications are filed on selected
inventions to extend market coverage
for companies and may also be available
for licensing.
FOR FURTHER INFORMATION CONTACT:
Licensing information and copies of the
U.S. patent applications listed below
may be obtained by writing to the
indicated licensing contact at the Office
of Technology Transfer, National
Institutes of Health, 6011 Executive
Boulevard, Suite 325, Rockville,
Maryland 20852–3804; telephone: 301–
496–7057; fax: 301–402–0220. A signed
Confidential Disclosure Agreement will
be required to receive copies of the
patent applications.
Monoclonal Antibodies That Recognize
the Human Type I Interferon Receptor
and Block Interferon Signaling
Description of Technology: Type I
interferons play a critical role in both
innate and adaptive immunity through
the stimulation of the IFNAR1 which
initiates interferon signaling in response
to viral and bacterial infections.
However, abnormal interferon signaling
is associated with human diseases, such
as lupus. The present invention
discloses six hybridomas that produce
mouse monoclonal antibodies specific
for the extracellular domain of human
IFNAR1. Two of the monoclonal
antibodies are able to bind IFNAR1 and
reduce interferon signaling. As such,
they can be utilized as a research tool
for studying the expression of IFNAR1
and the inhibition of IFNAR1 function
in humans or possibly as therapeutic
reagents for human diseases.
Potential Commercial Applications:
• Research reagents for studying the
expression and signaling of IFNAR1.
• A potential therapeutic reagent.
Competitive Advantages:
• Specific for the extracellular
domain of human IFNAR1. Can
therefore specifically recognize receptor
expressed on the cell surface.
• Bind IFNAR1 and reduce interferon
signaling
Development Stage:
• Pilot
• In vitro data available
Inventors: Sonja M. Best, Kirk Lubick,
Shelly J. Robertson (NIAID)
Publications:
1. Goldman LA, et al. Characterization
of antihuman IFNAR–1 monoclonal
antibodies: epitope localization and
functional analysis. J Interferon
Cytokine Res. 1999 Jan;19(1):15–26.
[PMID 10048764]
VerDate Mar<15>2010
15:54 Aug 26, 2013
Jkt 229001
2. Benoit P, et al. A monoclonal
antibody to recombinant human IFNalpha receptor inhibits biologic activity
of several species of human IFN-alpha,
IFN-beta, and IFN-omega. Detection of
heterogeneity of the cellular type I IFN
receptor. J Immunol. 1993 Feb
1;150(3):707–16. [PMID 8423335]
Intellectual Property: HHS Reference
No. E–527–2013/0—Research Material.
Patent protection is not being pursued
for this technology.
Licensing Contact: Susan Ano, Ph.D.;
301–435–5515; anos@mail.nih.gov.
Collaborative Research Opportunity:
The National Institute of Allergy and
Infectious Diseases (NIAID) is seeking
statements of capability or interest from
parties interested in collaborative
research to further develop, evaluate or
commercialize human type I interferon
receptor antibodies. For collaboration
opportunities, please contact Alicia
Evangelista at alicia.evangelista@
nih.gov or 301–594–1673.
Anthrax Fusion Toxins With Improved
Ability To Penetrate Cells
Description of Technology: Available
for licensing are novel conjugated or
fusion proteins comprised of anthrax
toxin lethal factor cytolethal distending
toxin subunit B. Several human tumor
cell lines have been found to be highly
sensitive to these toxins with LD50
values in the pM range. In vivo studies
in mice have revealed that these toxins
selectively treat tumors and have very
low systemic toxicity.
Potential Commercial Applications:
• Pharmaceutical compositions to
selectively treat cancer
• Applications to treat or prevent
growth of undesirable cells
Competitive Advantages:
• Selective with low systemic toxicity
• Potent (pM LD50 values)
Development Stage:
• Early-stage
• In vitro data available
• In vivo data available (animal)
Inventors: Christopher Bachran and
Stephen Leppla (NIAID)
Intellectual Property: HHS Reference
No. E–120–2013/0—US Application No.
61/837,428 filed June 20, 2013
Licensing Contact: Patrick McCue,
Ph.D.; 301–435–5560; mccuepat@
mail.nih.gov.
Method and Platform for Selectively
Labeling RNA
Description of Technology: The
invention pertains to a three step
initiation, elongation and termination
method and platform for synthesizing
selectively labeled RNA molecules by
first polymerizing a first liquid phase
RNA molecule from a solid phased DNA
PO 00000
Frm 00040
Fmt 4703
Sfmt 4703
52935
template fixed onto a solid phase. The
method includes the steps of incubating
the solid and liquid phases at
appropriate elongation temperatures
and then terminating elongation by a
separation stage where the phases are
incubated at near 0 degrees Celsius
where it selectively terminates RNA
elongation. The steps can be repeated by
the number bases (rNTPs) in the final
RNA molecule wherein in each iterative
stage a new rNTP can be added that is
selectively labeled. The DNA may have
a density of 30–80% on the solid
substrate, and the solid substrate may be
a bead. The bead may comprise a gel,
glass, or a synthetic polymer. The bead
may have a diameter of 5–100 mm. The
concentration of DNA may be 30 mm–
1 nm. The concentration of rNTP may
be 1–100 times the DNA concentration.
The RNA polymerase may be a T7 RNA
polymerase. The label may be
13C/5N, 2H, Cy3, Cy5, a fluorophore, a
heavy atom, or a chemical modification.
Potential Commercial Applications:
Differentially labeled diagnostics
Competitive Advantages: Multiple use
detection method
Development Stage:
• Prototype
• In vitro data available
Inventors: Yun-Xing Wang (NCI), Liu
Yu (NCI), Ping Yu (NCI), Rui Sousa
(Univ. Texas Health Science Ctr)
Publications:
1. Guajardo R, Sousa R. A model for
the mechanism of polymerase
translocation. J Mol Biol. 1997 Jan
10;265(1):8–19. [PMID 8995520]
2. Guo Q, et al. (2005). Major
conformational changes during T7RNAP
transcription initiation coincide with,
and are required for, promoter release.
J Mol Biol. 2005 Oct 21;353(2):256–70.
[PMID 16169559]
3. Mukherjee S, et al. Structural
transitions mediating transcription
initiation by T7 RNA polymerase. Cell.
2002 Jul 12;110(1):81–91. [PMID
12150999]
4. Mentesana PE, et al.
Characterization of halted T7 RNA
polymerase elongation complexes
reveals multiple factors that contribute
to stability. J Mol Biol. 2000 Oct
6;302(5):1049–62. [PMID 11183774]
Intellectual Property: HHS Reference
No. E–119–2013/0—US Provisional
Patent Application No. 61/843,864 filed
July 8, 2013
Licensing Contact: Michael
Shmilovich, Esq., CLP; 301–435–5019;
shmilovm@mail.nih.gov.
E:\FR\FM\27AUN1.SGM
27AUN1
52936
Federal Register / Vol. 78, No. 166 / Tuesday, August 27, 2013 / Notices
Vaccine Adjuvant for Inducing Th17
Focused Response
tkelley on DSK3SPTVN1PROD with NOTICES
Blood-Based Assay for the Diagnosis
and Monitoring of Hyposialylation
Disorders
Description of Technology: Sialic
acid, a monosaccharide widely
distributed in glycoproteins and
glycolipids, plays an important role in
biological processes such as cellular
adhesion, cellular communication and
signal transduction. Reduced levels of
sialic acid in tissues (also known as
hyposialylation) affect the function of
muscle, kidney, and other organ
systems, and are found in a number of
disorders, such as hereditary inclusion
body myopathy (HIBM, also known as
GNE myopathy), renal hyposialylation
disorders, and congenital disorders of
glycosylation.
The inventors have developed a
sensitive, reliable assay for the diagnosis
of hyposialylation disorders that detects
a novel glycoprotein biomarker in a
patient blood sample. This assay has
been validated using samples from
patients with GNE myopathy and other
hyposialylation disorders. A distinct
advantage of this assay is that it is
minimally invasive, unlike many
currently-available methods for
diagnosing hyposialylation disorders,
which typically require a tissue biopsy.
In particular, this biomarker represents
the first non-invasive method for
diagnosis of renal hyposialylation.
Potential Commercial Applications:
• Diagnostic assay to detect
hyposialylation
• Monitoring tool to track patient
response to sialylation-increasing
therapy
Competitive Advantages: A bloodbased assay based on this technology
would be less invasive, time-consuming,
and costly than a tissue biopsy, which
is the current diagnostic standard for
hyposialylation disorders, particularly
kidney disorders.
Development Stage:
• Early-stage
• In vitro data available
Inventors: Marjan Huizing (NHGRI),
William Gahl (NHGRI), Nuria CarrilloCarrasco (NCATS)
Intellectual Property: HHS Reference
No. E–056–2013/0—U.S. Application
No. 61/785,094 filed 14 Mar 2013
Related Technologies:
• HHS Reference No. E–217–2007/
0—N-Acetyl Mannosamine as a
Therapeutic Agent
• HHS Reference No. E–270–2011/
0—Encapsulated N-Acetylmannosamine
or N-Acetylneuraminic Acid to Increase
Sialylation
Licensing Contact: Tara Kirby, Ph.D.;
301–435–4426; tarak@mail.nih.gov.
VerDate Mar<15>2010
15:54 Aug 26, 2013
Jkt 229001
Description of Technology: Adjuvant
selection can be critical to a vaccine’s
effectiveness. Ideally, an adjuvant will
target and activate specific immune
pathways to increase the magnitude of
a response to the vaccine. A limited
range of adjuvants are presently
available for human clinical use; these
primarily affect T helper cells 1 and 2
(Th1 and Th2). Currently, no adjuvants
are approved for human use which
primarily affect IL–17-producing T
helper cells (Th17) cells. Th17 focused
adjuvants may prove critical for
developing operative vaccines against
pathogens where Th17 activity is
essential for protection. This technology
relates to novel adjuvants activating
either caspase-associated recruitment
domain protein 9 (CARD9) or caspase 1
pathways, or a combination of the two;
and methods for using these adjuvants
for stimulating an immune response.
These adjuvants induce Th17 focused
stimulation, which may prove essential
to development of effective vaccines
against a range of pathogens including
bacteria and fungi.
Potential Commercial Applications:
Vaccine
Competitive Advantages: Th17
skewing adjuvant
Development Stage: Early-stage
Inventors: Alan Sher (NIAID), Kevin
Shenderov (NIAID), Vincenzo
Cerundolo (University of Oxford, U.K.),
Gurdyal Besra (University of
Birmingham, U.K.)
Publication: Shenderov K, et al. Cord
factor and peptidoglycan recapitulate
the Th17-promoting adjuvant activity of
mycobacteria through mincle/CARD9
signaling and the inflammasome. J
Immunol. 2013 Jun 1;190(11):5722–30.
[PMID 23630357]
Intellectual Property: HHS Reference
No. E–089–2012/0—U.S. Provisional
Patent Application No. 61/709,713 filed
October 4, 2012
Licensing Contact: Edward (Tedd)
Fenn, J.D.; 424–500–2005; Tedd.fenn@
nih.gov.
Collaborative Research Opportunity:
The National Institute of Allergy and
Infectious Diseases is seeking statements
of capability or interest from parties
interested in collaborative research to
further develop, evaluate or
commercialize this technology. For
collaboration opportunities, please
contact Richard Kitei at 301–496–2644.
PO 00000
Frm 00041
Fmt 4703
Sfmt 4703
Dated: August 22, 2013.
Richard U. Rodriguez,
Director, Division of Technology Development
and Transfer, Office of Technology Transfer,
National Institutes of Health.
[FR Doc. 2013–20888 Filed 8–26–13; 8:45 am]
BILLING CODE 4140–01–P
DEPARTMENT OF HEALTH AND
HUMAN SERVICES
National Institutes of Health
Center For Scientific Review; Notice of
Closed Meetings
Pursuant to section 10(d) of the
Federal Advisory Committee Act, as
amended (5 U.S.C. App.), notice is
hereby given of the following meetings.
The meetings will be closed to the
public in accordance with the
provisions set forth in sections
552b(c)(4) and 552b(c)(6), Title 5 U.S.C.,
as amended. The grant applications and
the discussions could disclose
confidential trade secrets or commercial
property such as patentable material,
and personal information concerning
individuals associated with the grant
applications, the disclosure of which
would constitute a clearly unwarranted
invasion of personal privacy.
Name of Committee: Center for Scientific
Review Special Emphasis Panel; AREA:
Oncological Sciences Grant Application.
Date: September 20, 2013.
Time: 10:00 a.m. to 4:00 p.m.
Agenda: To review and evaluate grant
applications.
Place: National Institutes of Health, 6701
Rockledge Drive, Bethesda, MD 20892.
Contact Person: Denise R Shaw, Ph.D.,
Scientific Review Officer, Center for
Scientific Review, National Institutes of
Health, 6701 Rockledge Drive, Room 6158,
MSC 7804, Bethesda, MD 20892, 301–435–
0198, shawdeni@csr.nih.gov.
Name of Committee: Center for Scientific
Review Special Emphasis Panel; PAR 13–
008: Shared Instrumentation: Confocal
Microscopy and Imaging.
Date: September 26, 2013.
Time: 8:00 a.m. to 8:00 p.m.
Agenda: To review and evaluate grant
applications.
Place: Hyatt Regency Bethesda, One
Bethesda Metro Center, 7400 Wisconsin
Avenue, Bethesda, MD 20814.
Contact Person: Elena Smirnova, Ph.D.,
Scientific Review Officer, Center for
Scientific Review, National Institutes of
Health, 6701 Rockledge Drive, Room 5187,
MSC 7840, Bethesda, MD 20892, 301–435–
1236, smirnove@csr.nih.gov.
(Catalogue of Federal Domestic Assistance
Program Nos. 93.306, Comparative Medicine;
93.333, Clinical Research, 93.306, 93.333,
93.337, 93.393–93.396, 93.837–93.844,
93.846–93.878, 93.892, 93.893, National
Institutes of Health, HHS)
E:\FR\FM\27AUN1.SGM
27AUN1
Agencies
[Federal Register Volume 78, Number 166 (Tuesday, August 27, 2013)]
[Notices]
[Pages 52934-52936]
From the Federal Register Online via the Government Printing Office [www.gpo.gov]
[FR Doc No: 2013-20888]
-----------------------------------------------------------------------
DEPARTMENT OF HEALTH AND HUMAN SERVICES
National Institutes of Health
Government-Owned Inventions; Availability for Licensing
AGENCY: National Institutes of Health, HHS.
ACTION: Notice.
-----------------------------------------------------------------------
SUMMARY: The inventions listed below are owned by an agency of the U.S.
Government and are available for
[[Page 52935]]
licensing in the U.S. in accordance with 35 U.S.C. 209 and 37 CFR Part
404 to achieve expeditious commercialization of results of federally-
funded research and development. Foreign patent applications are filed
on selected inventions to extend market coverage for companies and may
also be available for licensing.
FOR FURTHER INFORMATION CONTACT: Licensing information and copies of
the U.S. patent applications listed below may be obtained by writing to
the indicated licensing contact at the Office of Technology Transfer,
National Institutes of Health, 6011 Executive Boulevard, Suite 325,
Rockville, Maryland 20852-3804; telephone: 301-496-7057; fax: 301-402-
0220. A signed Confidential Disclosure Agreement will be required to
receive copies of the patent applications.
Monoclonal Antibodies That Recognize the Human Type I Interferon
Receptor and Block Interferon Signaling
Description of Technology: Type I interferons play a critical role
in both innate and adaptive immunity through the stimulation of the
IFNAR1 which initiates interferon signaling in response to viral and
bacterial infections. However, abnormal interferon signaling is
associated with human diseases, such as lupus. The present invention
discloses six hybridomas that produce mouse monoclonal antibodies
specific for the extracellular domain of human IFNAR1. Two of the
monoclonal antibodies are able to bind IFNAR1 and reduce interferon
signaling. As such, they can be utilized as a research tool for
studying the expression of IFNAR1 and the inhibition of IFNAR1 function
in humans or possibly as therapeutic reagents for human diseases.
Potential Commercial Applications:
Research reagents for studying the expression and
signaling of IFNAR1.
A potential therapeutic reagent.
Competitive Advantages:
Specific for the extracellular domain of human IFNAR1. Can
therefore specifically recognize receptor expressed on the cell
surface.
Bind IFNAR1 and reduce interferon signaling
Development Stage:
Pilot
In vitro data available
Inventors: Sonja M. Best, Kirk Lubick, Shelly J. Robertson (NIAID)
Publications:
1. Goldman LA, et al. Characterization of antihuman IFNAR-1
monoclonal antibodies: epitope localization and functional analysis. J
Interferon Cytokine Res. 1999 Jan;19(1):15-26. [PMID 10048764]
2. Benoit P, et al. A monoclonal antibody to recombinant human IFN-
alpha receptor inhibits biologic activity of several species of human
IFN-alpha, IFN-beta, and IFN-omega. Detection of heterogeneity of the
cellular type I IFN receptor. J Immunol. 1993 Feb 1;150(3):707-16.
[PMID 8423335]
Intellectual Property: HHS Reference No. E-527-2013/0--Research
Material. Patent protection is not being pursued for this technology.
Licensing Contact: Susan Ano, Ph.D.; 301-435-5515;
anos@mail.nih.gov.
Collaborative Research Opportunity: The National Institute of
Allergy and Infectious Diseases (NIAID) is seeking statements of
capability or interest from parties interested in collaborative
research to further develop, evaluate or commercialize human type I
interferon receptor antibodies. For collaboration opportunities, please
contact Alicia Evangelista at alicia.evangelista@nih.gov or 301-594-
1673.
Anthrax Fusion Toxins With Improved Ability To Penetrate Cells
Description of Technology: Available for licensing are novel
conjugated or fusion proteins comprised of anthrax toxin lethal factor
cytolethal distending toxin subunit B. Several human tumor cell lines
have been found to be highly sensitive to these toxins with LD50 values
in the pM range. In vivo studies in mice have revealed that these
toxins selectively treat tumors and have very low systemic toxicity.
Potential Commercial Applications:
Pharmaceutical compositions to selectively treat cancer
Applications to treat or prevent growth of undesirable
cells
Competitive Advantages:
Selective with low systemic toxicity
Potent (pM LD50 values)
Development Stage:
Early-stage
In vitro data available
In vivo data available (animal)
Inventors: Christopher Bachran and Stephen Leppla (NIAID)
Intellectual Property: HHS Reference No. E-120-2013/0--US
Application No. 61/837,428 filed June 20, 2013
Licensing Contact: Patrick McCue, Ph.D.; 301-435-5560;
mccuepat@mail.nih.gov.
Method and Platform for Selectively Labeling RNA
Description of Technology: The invention pertains to a three step
initiation, elongation and termination method and platform for
synthesizing selectively labeled RNA molecules by first polymerizing a
first liquid phase RNA molecule from a solid phased DNA template fixed
onto a solid phase. The method includes the steps of incubating the
solid and liquid phases at appropriate elongation temperatures and then
terminating elongation by a separation stage where the phases are
incubated at near 0 degrees Celsius where it selectively terminates RNA
elongation. The steps can be repeated by the number bases (rNTPs) in
the final RNA molecule wherein in each iterative stage a new rNTP can
be added that is selectively labeled. The DNA may have a density of 30-
80% on the solid substrate, and the solid substrate may be a bead. The
bead may comprise a gel, glass, or a synthetic polymer. The bead may
have a diameter of 5-100 mm. The concentration of DNA may be 30 mm-1
nm. The concentration of rNTP may be 1-100 times the DNA concentration.
The RNA polymerase may be a T7 RNA polymerase. The label may be \13\C/
\5\N, \2\H, Cy3, Cy5, a fluorophore, a heavy atom, or a chemical
modification.
Potential Commercial Applications: Differentially labeled
diagnostics
Competitive Advantages: Multiple use detection method
Development Stage:
Prototype
In vitro data available
Inventors: Yun-Xing Wang (NCI), Liu Yu (NCI), Ping Yu (NCI), Rui
Sousa (Univ. Texas Health Science Ctr)
Publications:
1. Guajardo R, Sousa R. A model for the mechanism of polymerase
translocation. J Mol Biol. 1997 Jan 10;265(1):8-19. [PMID 8995520]
2. Guo Q, et al. (2005). Major conformational changes during T7RNAP
transcription initiation coincide with, and are required for, promoter
release. J Mol Biol. 2005 Oct 21;353(2):256-70. [PMID 16169559]
3. Mukherjee S, et al. Structural transitions mediating
transcription initiation by T7 RNA polymerase. Cell. 2002 Jul
12;110(1):81-91. [PMID 12150999]
4. Mentesana PE, et al. Characterization of halted T7 RNA
polymerase elongation complexes reveals multiple factors that
contribute to stability. J Mol Biol. 2000 Oct 6;302(5):1049-62. [PMID
11183774]
Intellectual Property: HHS Reference No. E-119-2013/0--US
Provisional Patent Application No. 61/843,864 filed July 8, 2013
Licensing Contact: Michael Shmilovich, Esq., CLP; 301-435-5019;
shmilovm@mail.nih.gov.
[[Page 52936]]
Blood-Based Assay for the Diagnosis and Monitoring of Hyposialylation
Disorders
Description of Technology: Sialic acid, a monosaccharide widely
distributed in glycoproteins and glycolipids, plays an important role
in biological processes such as cellular adhesion, cellular
communication and signal transduction. Reduced levels of sialic acid in
tissues (also known as hyposialylation) affect the function of muscle,
kidney, and other organ systems, and are found in a number of
disorders, such as hereditary inclusion body myopathy (HIBM, also known
as GNE myopathy), renal hyposialylation disorders, and congenital
disorders of glycosylation.
The inventors have developed a sensitive, reliable assay for the
diagnosis of hyposialylation disorders that detects a novel
glycoprotein biomarker in a patient blood sample. This assay has been
validated using samples from patients with GNE myopathy and other
hyposialylation disorders. A distinct advantage of this assay is that
it is minimally invasive, unlike many currently-available methods for
diagnosing hyposialylation disorders, which typically require a tissue
biopsy. In particular, this biomarker represents the first non-invasive
method for diagnosis of renal hyposialylation.
Potential Commercial Applications:
Diagnostic assay to detect hyposialylation
Monitoring tool to track patient response to sialylation-
increasing therapy
Competitive Advantages: A blood-based assay based on this
technology would be less invasive, time-consuming, and costly than a
tissue biopsy, which is the current diagnostic standard for
hyposialylation disorders, particularly kidney disorders.
Development Stage:
Early-stage
In vitro data available
Inventors: Marjan Huizing (NHGRI), William Gahl (NHGRI), Nuria
Carrillo-Carrasco (NCATS)
Intellectual Property: HHS Reference No. E-056-2013/0--U.S.
Application No. 61/785,094 filed 14 Mar 2013
Related Technologies:
HHS Reference No. E-217-2007/0--N-Acetyl Mannosamine as a
Therapeutic Agent
HHS Reference No. E-270-2011/0--Encapsulated N-
Acetylmannosamine or N-Acetylneuraminic Acid to Increase Sialylation
Licensing Contact: Tara Kirby, Ph.D.; 301-435-4426;
tarak@mail.nih.gov.
Vaccine Adjuvant for Inducing Th17 Focused Response
Description of Technology: Adjuvant selection can be critical to a
vaccine's effectiveness. Ideally, an adjuvant will target and activate
specific immune pathways to increase the magnitude of a response to the
vaccine. A limited range of adjuvants are presently available for human
clinical use; these primarily affect T helper cells 1 and 2 (Th1 and
Th2). Currently, no adjuvants are approved for human use which
primarily affect IL-17-producing T helper cells (Th17) cells. Th17
focused adjuvants may prove critical for developing operative vaccines
against pathogens where Th17 activity is essential for protection. This
technology relates to novel adjuvants activating either caspase-
associated recruitment domain protein 9 (CARD9) or caspase 1 pathways,
or a combination of the two; and methods for using these adjuvants for
stimulating an immune response. These adjuvants induce Th17 focused
stimulation, which may prove essential to development of effective
vaccines against a range of pathogens including bacteria and fungi.
Potential Commercial Applications: Vaccine
Competitive Advantages: Th17 skewing adjuvant
Development Stage: Early-stage
Inventors: Alan Sher (NIAID), Kevin Shenderov (NIAID), Vincenzo
Cerundolo (University of Oxford, U.K.), Gurdyal Besra (University of
Birmingham, U.K.)
Publication: Shenderov K, et al. Cord factor and peptidoglycan
recapitulate the Th17-promoting adjuvant activity of mycobacteria
through mincle/CARD9 signaling and the inflammasome. J Immunol. 2013
Jun 1;190(11):5722-30. [PMID 23630357]
Intellectual Property: HHS Reference No. E-089-2012/0--U.S.
Provisional Patent Application No. 61/709,713 filed October 4, 2012
Licensing Contact: Edward (Tedd) Fenn, J.D.; 424-500-2005;
Tedd.fenn@nih.gov.
Collaborative Research Opportunity: The National Institute of
Allergy and Infectious Diseases is seeking statements of capability or
interest from parties interested in collaborative research to further
develop, evaluate or commercialize this technology. For collaboration
opportunities, please contact Richard Kitei at 301-496-2644.
Dated: August 22, 2013.
Richard U. Rodriguez,
Director, Division of Technology Development and Transfer, Office of
Technology Transfer, National Institutes of Health.
[FR Doc. 2013-20888 Filed 8-26-13; 8:45 am]
BILLING CODE 4140-01-P