Trichoderma reesei; Proposed Significant New Use Rule, 35331-35336 [2012-14242]
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Federal Register / Vol. 77, No. 114 / Wednesday, June 13, 2012 / Proposed Rules
affect small governments, as described
in the Unfunded Mandates Reform Act
of 1995 (Public Law 104–4);
• Does not have Federalism
implications as specified in Executive
Order 13132 (64 FR 43255, August 10,
1999);
• Is not an economically significant
regulatory action based on health or
safety risks subject to Executive Order
13045 (62 FR 19885, April 23, 1997);
• Is not a significant regulatory action
subject to Executive Order 13211 (66 FR
28355, May 22, 2001);
• Is not subject to requirements of
Section 12(d) of the National
Technology Transfer and Advancement
Act of 1995 (15 U.S.C. 272 note) because
application of those requirements would
be inconsistent with the Clean Air Act;
and
• Does not provide EPA with the
discretionary authority to address
disproportionate human health or
environmental effects with practical,
appropriate, and legally permissible
methods under Executive Order 12898
(59 FR 7629, February 16, 1994).
In addition, this proposed action does
not have tribal implications as specified
by Executive Order 13175 (65 FR 67249,
November 9, 2000), because the SIP is
not approved to apply in Indian country
located in the State, and EPA notes that
it will not impose substantial direct
costs on tribal governments or preempt
tribal law.
List of Subjects in 40 CFR Part 52
Environmental protection, Air
pollution control, Intergovernmental
relations, Ozone, Reporting and
recordkeeping requirements, Volatile
organic compounds.
Authority: 42 U.S.C. 7401 et seq.
Dated: May 25, 2012.
Jared Blumenfeld,
Regional Administrator, Region IX.
[FR Doc. 2012–14410 Filed 6–12–12; 8:45 am]
BILLING CODE 6560–50–P
ENVIRONMENTAL PROTECTION
AGENCY
40 CFR Part 725
[EPA–HQ–OPPT–2010–0994; FRL–9350–6]
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RIN 2070–AD43
Trichoderma reesei; Proposed
Significant New Use Rule
Environmental Protection
Agency (EPA).
ACTION: Proposed rule.
AGENCY:
EPA is proposing a significant
new use rule (SNUR) under the Toxic
SUMMARY:
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Substances Control Act (TSCA) for the
genetically modified microorganism
identified generically as Trichoderma
reesei (T. reesei). This microorganism
was the subject of a Microbial
Commercial Activity Notice (MCAN).
EPA believes this action is necessary
because the use of this genetically
modified T. reesei under certain
conditions may be hazardous to human
health and the environment. This
proposed rule would also establish a
mechanism to allow EPA to evaluate an
intended use and its conditions, and to
prohibit or limit that activity before it
occurs, if EPA determines it may be
hazardous.
DATES: Comments must be received on
or before July 13, 2012.
ADDRESSES: Submit your comments,
identified by docket identification (ID)
number EPA–HQ–OPPT–2010–0994, by
one of the following methods:
• Federal eRulemaking Portal: https://
www.regulations.gov. Follow the online
instructions for submitting comments.
• Mail: Document Control Office
(7407M), Office of Pollution Prevention
and Toxics (OPPT), Environmental
Protection Agency, 1200 Pennsylvania
Ave. NW., Washington, DC 20460–0001.
• Hand Delivery: OPPT Document
Control Office (DCO), EPA East Bldg.,
Rm. 6428, 1201 Constitution Ave. NW.,
Washington, DC. Attention: Docket ID
Number EPA–HQ–OPPT–2010–0994.
The DCO is open from 8 a.m. to 4 p.m.,
Monday through Friday, excluding legal
holidays. The telephone number for the
DCO is (202) 564–8930. Such deliveries
are only accepted during the DCO’s
normal hours of operation, and special
arrangements should be made for
deliveries of boxed information.
Instructions: Direct your comments to
docket ID number EPA–HQ–OPPT–
2010–0994. EPA’s policy is that all
comments received will be included in
the docket without change and may be
made available online at https://
www.regulations.gov, including any
personal information provided, unless
the comment includes information
claimed to be Confidential Business
Information (CBI) or other information
whose disclosure is restricted by statute.
Do not submit information that you
consider to be CBI or otherwise
protected through regulations.gov or
email. The regulations.gov Web site is
an ‘‘anonymous access’’ system, which
means EPA will not know your identity
or contact information unless you
provide it in the body of your comment.
If you send an email comment directly
to EPA without going through
regulations.gov, your email address will
be automatically captured and included
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35331
as part of the comment that is placed in
the docket and made available on the
Internet. If you submit an electronic
comment, EPA recommends that you
include your name and other contact
information in the body of your
comment and with any disk or CD–ROM
you submit. If EPA cannot read your
comment due to technical difficulties
and cannot contact you for clarification,
EPA may not be able to consider your
comment. Electronic files should avoid
the use of special characters, any form
of encryption, and be free of any defects
or viruses.
Docket: All documents in the docket
are listed in the docket index available
at https://www.regulations.gov. Although
listed in the index, some information is
not publicly available, e.g., CBI or other
information whose disclosure is
restricted by statute. Certain other
material, such as copyrighted material,
will be publicly available only in hard
copy. Publicly available docket
materials are available electronically at
https://www.regulations.gov, or, if only
available in hard copy, at the OPPT
Docket. The OPPT Docket is located in
the EPA Docket Center (EPA/DC) at Rm.
3334, EPA West Bldg., 1301
Constitution Ave. NW., Washington,
DC. The EPA/DC Public Reading Room
hours of operation are 8:30 a.m. to 4:30
p.m., Monday through Friday, excluding
legal holidays. The telephone number of
the EPA/DC Public Reading Room is
(202) 566–1744, and the telephone
number for the OPPT Docket is (202)
566–0280. Docket visitors are required
to show photographic identification,
pass through a metal detector, and sign
the EPA visitor log. All visitor bags are
processed through an X-ray machine
and subject to search. Visitors will be
provided an EPA/DC badge that must be
visible at all times in the building and
returned upon departure.
For
technical information contact: Kenneth
Moss, Chemical Control Division
(7405M), Office of Pollution Prevention
and Toxics, Environmental Protection
Agency, 1200 Pennsylvania Ave. NW.,
Washington, DC 20460–0001; telephone
number: (202) 564–9232; email address:
moss.kenneth@epa.gov.
For general information contact: The
TSCA-Hotline, ABVI-Goodwill, 422
South Clinton Ave., Rochester, NY
14620; telephone number: (202) 554–
1404; email address: TSCAHotline@epa.gov.
FOR FURTHER INFORMATION CONTACT:
SUPPLEMENTARY INFORMATION:
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I. General Information
A. Does this action apply to me?
You may be potentially affected by
this action if you manufacture, import,
process, or use products that contain
living microorganisms subject to TSCA,
especially if you know that your
products contain or may contain T.
reesei. Potentially affected entities may
include, but are not limited to:
• Manufacturers, importers, or
processors of chemical substances
(NAICS codes 325 and 324110), e.g.,
chemical manufacturing and petroleum
refineries.
This listing is not intended to be
exhaustive, but rather provides a guide
for readers regarding entities likely to be
affected by this action. Other types of
entities not listed in this unit could also
be affected. The North American
Industrial Classification System
(NAICS) codes have been provided to
assist you and others in determining
whether this action might apply to
certain entities. To determine whether
you or your business may be affected by
this action, you should carefully
examine the list of chemical substances
excluded by TSCA section 3(2)(B) and
the applicability provisions in
§ 725.105(c) for SNUR related
obligations. If you have any questions
regarding the applicability of this action
to a particular entity, consult the
technical person listed under FOR
FURTHER INFORMATION CONTACT.
This action may also affect certain
entities through pre-existing import
certification and export notification
rules under TSCA. Chemical importers
are subject to the TSCA section 13 (15
U.S.C. 2612) import certification
requirements promulgated at 19 CFR
12.118 through 12.127; see also 19 CFR
127.28. Chemical importers must certify
that the shipment of the chemical
substance complies with all applicable
rules and orders under TSCA. Importers
of chemical substances subject to these
SNURs must certify their compliance
with the SNUR requirements. The EPA
policy in support of import certification
appears at 40 CFR part 707, subpart B.
In addition, any persons who export or
intend to export a chemical substance
that is the subject of a proposed or final
SNUR are subject to the export
notification provisions of TSCA section
12(b) (15 U.S.C. 2611(b)) (see
§ 725.920), and must comply with the
export notification requirements in 40
CFR part 707, subpart D.
B. What should I consider as I prepare
my comments for EPA?
1. Submitting CBI. Do not submit this
information to EPA through
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regulations.gov or email. Clearly mark
the part or all of the information that
you claim to be CBI. For CBI
information in a disk or CD–ROM that
you mail to EPA, mark the outside of the
disk or CD–ROM as CBI and then
identify electronically within the disk or
CD–ROM the specific information that
is claimed as CBI. In addition to one
complete version of the comment that
includes information claimed as CBI, a
copy of the comment that does not
contain the information claimed as CBI
must be submitted for inclusion in the
public docket. Information so marked
will not be disclosed except in
accordance with procedures set forth in
40 CFR part 2.
2. Tips for preparing your comments.
When submitting comments, remember
to:
i. Identify the document by docket ID
number and other identifying
information (subject heading, Federal
Register date and page number).
ii. Follow directions. The Agency may
ask you to respond to specific questions
or organize comments by referencing a
Code of Federal Regulations (CFR) part
or section number.
iii. Explain why you agree or disagree;
suggest alternatives and substitute
language for your requested changes.
iv. Describe any assumptions and
provide any technical information and/
or data that you used.
v. If you estimate potential costs or
burdens, explain how you arrived at
your estimate in sufficient detail to
allow for it to be reproduced.
vi. Provide specific examples to
illustrate your concerns and suggest
alternatives.
vii. Explain your views as clearly as
possible, avoiding the use of profanity
or personal threats.
viii. Make sure to submit your
comments by the comment period
deadline identified.
II. Background
A. What action is the agency taking?
EPA is proposing this SNUR for the
genetically modified microorganism
identified generically as T. reesei
(MCAN J–10–2). This proposed rule
would require persons to notify EPA at
least 90 days before commencing the
manufacture, import, or processing of
the microorganism for any activity
designated as a significant new use.
B. What is the agency’s authority for
taking this action?
Section 5(a)(2) of TSCA (15 U.S.C.
2604(a)(2)) authorizes EPA to determine
that a use of a chemical substance is a
‘‘significant new use.’’ (see 40 CFR part
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725, subparts L and M). EPA must make
this determination by rule after
considering all relevant factors,
including the TSCA section 5(a)(2)
factors, listed in Unit III. Once EPA
determines that a use of a chemical
substance is a significant new use,
TSCA section 5(a)(1)(B) requires persons
to submit a significant new use notice
(SNUN) to EPA at least 90 days before
they manufacture, import, or process the
chemical substance for that use. Persons
who must report are described in
§ 725.105(c).
EPA has interpreted the TSCA section
3(2) definition of ‘‘chemical substance’’
as authorizing EPA to regulate
microorganisms under TSCA. See the
Federal Register issue of April 11, 1997
(62 FR 17910) (FRL–5577–2).
C. Applicability of General Provisions
General provisions for SNURs for
microorganisms appear in 40 CFR part
725, subpart L. These provisions
describe persons subject to the proposed
rule, recordkeeping requirements,
exemptions to reporting requirements,
and applicability to uses occurring
before the effective date of the final rule.
Provisions relating to user fees appear at
40 CFR part 700. Persons subject to this
SNUR must comply with the notice
requirements under TSCA section
5(a)(1)(A) and must submit a MCAN,
using the procedures set out in 40 CFR
part 725, subpart D, and additional
‘‘Significant New Uses of
Microorganisms’’ procedures at 40 CFR
part 725, subpart L.
Under 40 CFR part 725, EPA has
adopted a more narrow interpretation of
the TSCA section 5(h)(3) exemption for
small quantities used in research than it
has for other chemical substances under
40 CFR part 721. Under § 725.3, EPA
has defined small quantities solely for
research and development as
‘‘quantities of a microorganism
manufactured, imported, or processed
or proposed to be manufactured,
imported, or processed solely for
research and development that meet the
requirements of § 725.234.’’ Any other
research and development activity of a
microorganism subject to a SNUR must
comply with the TSCA section
5(a)(1)(A) notification requirements
unless that activity has been excluded
from coverage under the SNUR. See
§ 725.3, subparts E and F of 40 CFR part
725, and the April 11, 1997 Federal
Register document. Once EPA receives
a SNUN, EPA may take regulatory
action under TSCA section 5(e), 5(f), 6,
or 7 to control the activities for which
it has received the SNUN. If EPA does
not take action, EPA is required under
TSCA section 5(g) to explain in the
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Federal Register its reasons for not
taking action.
III. Significant New Use Determination
Section 5(a)(2) of TSCA states that
EPA’s determination that a use of a
chemical substance is a significant new
use must be made after consideration of
all relevant factors, including:
• The projected volume of
manufacturing and processing of a
chemical substance.
• The extent to which a use changes
the type or form of exposure of human
beings or the environment to a chemical
substance.
• The extent to which a use increases
the magnitude and duration of exposure
of human beings or the environment to
a chemical substance.
• The reasonably anticipated manner
and methods of manufacturing,
processing, distribution in commerce,
and disposal of a chemical substance.
In addition to these factors specifically
enumerated in TSCA section 5(a)(2), the
statute authorizes EPA to consider any
other relevant factors.
To determine what would constitute a
significant new use for the chemical
substance that is the subject of this
proposed SNUR, EPA considered the
available information relating to the four
bulleted factors listed in TSCA section
5(a)(2) factors listed in this unit, and
other relevant factors. This includes
relevant information about the toxicity
of the chemical substance and likely
human exposures and environmental
releases associated with possible uses.
See the risk assessment in the docket
under docket ID number EPA–HQ–
OPPT–2010–0994 for this information
and other relevant factors.
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IV. Substance Subject to This Proposed
Rule
EPA is proposing to establish
significant new use and recordkeeping
requirements for only the
microorganism identified generically as
T. reesei, genetically modified as
described in MCAN J–10–2. This will be
codified in 40 CFR part 725, subpart M.
Any T. reesei microorganism with
genetic modifications other than those
described in MCAN J–10–2 would not
be subject to this SNUR and will require
submission and EPA review of a
separate MCAN.
MCAN Number J–10–2
Chemical name: Trichoderma reesei
(MCAN J–10–2) (generic).
Chemical Abstracts Service (CAS)
Registry Number: Not available.
Use: The MCAN states that the
generic (non-confidential) use of the
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microorganism will be to produce
enzymes for ethanol production.
Basis for action: When used to
produce enzymes that can release sugars
from de-lignified plant materials,
human and environmental exposures to
live T. reesei cells are low, due to the
containment and inactivation
procedures specified in the MCAN.
These containment and inactivation
procedures are consistent with standard
industry practices and those delineated
in 40 CFR 725.422(d). These procedures
include the use of equipment to
minimize aerosol releases from the
facility, and the use of inactivation
methods that reduce the number of
viable cells by at least 6 logs (i.e., 106)
in the liquid and solid waste streams.
More importantly, the manufacturing
process described in the MCAN relies
on the typical submerged standard
industrial fermentation process for
enzyme production, wherein the
microorganism is grown in liquid broth
culture in the absence of solid materials
or solid surfaces, the fermentation is
terminated prior to the microorganism
entering the stationary phase of growth,
and the enzyme is separated from the
microbial biomass which is inactivated
prior to disposal. Therefore, EPA
determined that the proposed
manufacturing, processing, or use of the
microorganism as described in the
MCAN is not expected to present an
unreasonable risk. However, EPA has
determined that use of the
microorganism under other conditions
may result in adverse human health and
environmental effects. Specifically,
where growth on solid plant material or
insoluble substrate occurs, T. reesei has
been shown to produce a secondary
metabolite known as paracelsin, which
is a peptaibol. Peptaibols are small
linear peptides of 1,000–2,000 Daltons
characterized by a high content of the
non-proteinogenic amino acid alphaamino-isobutyric acid (Aib), with a Nterminus that is typically acetylated,
and a C-terminus that is linked to an
amino alcohol, which is usually
phenylalaninol, or sometimes valinol,
leucinol, isoleucinol, or tryptophanol.
Peptaibols are associated with a wide
variety of biological activities and have
antifungal, antibacterial, sometimes
antiviral, antiparasitic, and neurotoxic
activity. Paracelsin has been shown to
have toxicity toward mammalian cells
such as hemolytic activity on human
erythrocytes and cytotoxicity to rat
adrenal medulla PC12
(pheochromocytoma) cells. Paracelsin
has also been shown to exhibit
cytotoxicity to Gram-positive bacteria,
to human erythrocytes, and to other
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mammals such as aquatic indicator
species. Additional information relating
to the assessment of this chemical
substance and paracelsin, including a
sanitized EPA risk assessment and a list
of references used, is available in the
docket under docket ID number EPA–
HQ–OPPT–2010–0994.
Recommended testing: EPA has
determined that the results of the
following studies would help
characterize any potential human health
and environmental effects of the MCAN
substance:
1. Investigation of whether paracelsin
will be produced, and at what levels if
the genetically modified T. reesei is
grown on various plant biomass
materials for different durations under
various fermentation conditions in
cellulosic biomass facilities.
2. If paracelsin is produced, a study
of whether paracelsin would be
denatured/inactivated during
production and processing.
3. If paracelsin is released from the
facility, a study of whether paracelsin
would be degraded/inactivated during
wastewater treatment.
4. If released to the environment,
studies on the persistence, stability,
dissemination, accumulation, and the
potential resulting biological activity of
paracelsin with exposure to aquatic and
terrestrial organisms in the
environment.
5. Studies to determine the ability of
the MCAN microorganism to survive in
the environment relative to the survival
of the unmodified parent or recipient
strain, and to assess its competitiveness
with other fungi in the environment.
This study may require some
supplementation with one or more
carbon sources and the use of various
soil types.
6. A study to determine survival of
the fungus during an anaerobic
fermentation for production of ethanol
by an ethanologen, and survival of the
fungus during ethanol distillation or at
the distillation temperature for ethanol.
CFR citation: 40 CFR 725.1077.
V. Rationale and Objectives of the
Proposed Rule
A. Rationale
During review of the specific T. reesei,
modified as described in MCAN J–10–
2, EPA determined that certain
fermentation conditions, other than the
typical submerged standard industrial
fermentation process for enzyme
production described in Unit IV., could
result in increased exposures thereby
constituting a ‘‘significant new use.’’
Specifically, EPA is concerned that
where growth on solid plant material or
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insoluble substrate occurs, T. reesei has
been shown to produce a secondary
metabolite known as paracelsin, which
is associated with a variety of toxic
effects to mammalian and bacterial
cells. Use of the MCAN microorganism
without the specific containment or
inactivation controls listed in the
MCAN, described in Unit IV., may
result in adverse human health and
environmental effects. Based on the
descriptions of manufacturing,
processing, and use in the MCAN J–10–
2, the Agency believes that uses of the
organism covered by the proposed
definition of a significant new use are
not currently ongoing.
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B. Objectives
EPA is proposing this SNUR for a
chemical substance that has undergone
review to achieve the following
objectives with regard to the significant
new uses designated in this proposed
rule:
• EPA would receive notice of any
person’s intent to manufacture, import,
or process a listed chemical substance
for the described significant new use
before that activity begins.
• EPA would have an opportunity to
review and evaluate data submitted in a
SNUN before the notice submitter
begins manufacturing, importing, or
processing a listed chemical substance
for the described significant new use.
• EPA would be able to determine
whether regulation of prospective
manufacturers, importers, or processors
of a listed chemical substance is
warranted pursuant to TSCA sections
5(e), 5(f), 6, or 7, and impose any
necessary requirements before the
described significant new use of that
chemical substance occurs.
Issuance of a SNUR for a chemical
substance does not signify that the
chemical substance is listed on the
TSCA Chemical Substance Inventory
(TSCA Inventory). Guidance on how to
determine if a chemical substance is on
the TSCA Inventory is available
electronically at https://www.epa.gov/
opptintr/existingchemicals/pubs/
tscainventory/.
VI. Applicability of Proposed Rule to
Uses Occurring Before Effective Date of
the Final Rule
To establish a significant ‘‘new’’ use,
EPA must determine that the use is not
ongoing. EPA solicits comments on
whether any of the uses proposed as
significant new uses are ongoing.
As discussed in the Federal Register
issue of April 24, 1990 (55 FR 17376),
EPA has decided that the intent of
TSCA section 5(a)(1)(B) is best served by
designating a use as a significant new
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use as of the date of publication of the
proposed rule rather than as of the
effective date of the final rule. If uses
begun after publication of the proposed
rule were considered ongoing rather
than new, it would be difficult for EPA
to establish SNUR notice requirements
because a person could defeat the SNUR
by initiating the significant new use
before the proposed rule became final,
and then argue that the use was ongoing
before the effective date of the final rule.
Thus, any persons who begin
commercial manufacture, import, or
processing activities with the
microorganism that would be regulated
through this proposed rule will have to
cease any such activity before the
effective date of the final rule, if and
when finalized. To resume their
activities, these persons would have to
comply with all applicable SNUR notice
requirements and wait until the notice
review period, including all extensions,
expires.
EPA has promulgated provisions to
allow persons to comply with this
proposed SNUR before the effective
date. If a person were to meet the
conditions of advance compliance
under 40 CFR 725.912(a), the person
would be considered exempt from the
requirements of the SNUR.
VII. Test Data and Other Information
EPA recognizes that TSCA section 5
does not require developing any
particular test data before submission of
a SNUN. There are two exceptions:
1. Development of test data is
required where the chemical substance
subject to the SNUR is also subject to a
test rule under TSCA section 4 (see
TSCA section 5(b)(1)).
2. Development of test data may be
necessary where the chemical substance
has been listed under TSCA section
5(b)(4) (see TSCA section 5(b)(2)).
In the absence of a TSCA section 4
test rule or a TSCA section 5(b)(4)
listing covering the chemical substance,
persons are required only to submit test
data in their possession or control and
to describe any other data known to or
reasonably ascertainable by them (see 40
CFR 725.25(a)(2)). However, upon
review of MCANs and SNUNs, the
Agency has the authority to require
appropriate testing. In this case, EPA
recommends persons, before performing
any testing, to consult with the Agency
pertaining to protocol selection.
The recommended testing specified in
Unit IV. may not be the only means of
addressing the potential risks for the
chemical substance. However, SNUNs
submitted without any test data may
increase the likelihood that EPA will
respond by taking action under TSCA
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section 5(e), particularly if satisfactory
test results have not been obtained from
a prior submission. EPA recommends
that potential SNUN submitters contact
EPA early enough so that they will be
able to conduct the appropriate tests.
SNUN submitters should be aware
that EPA will be better able to evaluate
SNUNs which provide detailed
information on the following:
• Human exposure and
environmental release that may result
from the significant new use of the
chemical substance.
• Potential benefits of the chemical
substance.
• Information on risks posed by the
chemical substance compared to risks
posed by potential substitutes.
VIII. SNUN Submissions
Persons subject to this SNUR must
comply with the notice requirements
under TSCA section 5(a)(1)(A) and must
submit a MCAN, using the procedures
set out in 40 CFR part 725, subpart D,
and additional ‘‘Significant New Uses of
Microorganisms’’ procedures at 40 CFR
part 725, subpart L. SNUNs must be
submitted to EPA on EPA Form No.
6300–07, generated using e-PMN
software, and submitted to the Agency
in accordance with the procedures set
forth in 40 CFR 725.25 and 40 CFR
725.27. E–PMN software is available
electronically at https://www.epa.gov/
opptintr/newchems.
IX. Economic Analysis
EPA has evaluated the potential costs
of establishing SNUN requirements for
potential manufacturers, importers, and
processors of the chemical substance
subject to this proposed rule. EPA’s
complete Economic Analysis is
available in the docket under docket ID
number EPA–HQ–OPPT–2010–0994.
X. Statutory and Executive Order
Reviews
A. Executive Order 12866
This proposed rule would establish a
SNUR for a chemical substance that was
the subject of a MCAN. The Office of
Management and Budget (OMB) has
exempted these types of actions from
review under Executive Order 12866,
entitled ‘‘Regulatory Planning and
Review’’ (58 FR 51735, October 4, 1993).
B. Paperwork Reduction Act
According to the Paperwork
Reduction Act (PRA) (44 U.S.C. 3501 et
seq.), an agency may not conduct or
sponsor, and a person is not required to
respond to a collection of information
that requires OMB approval under PRA,
unless it has been approved by OMB
and displays a currently valid OMB
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control number. The OMB control
numbers for EPA’s regulations in title 40
of the CFR, after appearing in the
Federal Register, are listed in 40 CFR
part 9, and included on the related
collection instrument or form, if
applicable. EPA would amend the table
in 40 CFR part 9 to list the OMB
approval number for the information
collection requirements contained in
this proposed rule. This listing of the
OMB control numbers and their
subsequent codification in the CFR
satisfies the display requirements of
PRA and OMB’s implementing
regulations at 5 CFR part 1320.
The information collection
requirements related to this action have
already been approved by OMB
pursuant to PRA under OMB control
number 2070–0012 (EPA ICR No. 574).
This action would not impose any
burden requiring additional OMB
approval. If an entity were to submit a
SNUN to the Agency, the annual burden
is estimated to average between 30 and
170 hours per response. This burden
estimate includes the time needed to
review instructions, search existing data
sources, gather and maintain the data
needed, and complete, review, and
submit the required SNUN.
Send any comments about the
accuracy of the burden estimate, and
any suggested methods for minimizing
respondent burden, including through
the use of automated collection
techniques, to the Director, Collection
Strategies Division, Office of
Environmental Information (2822T),
Environmental Protection Agency, 1200
Pennsylvania Ave. NW., Washington,
DC 20460–0001. Please remember to
include the OMB control number in any
correspondence, but do not submit any
completed forms to this address.
C. Regulatory Flexibility Act
On February 18, 2012, EPA certified
pursuant to section 605(b) of the
Regulatory Flexibility Act (RFA) (5
U.S.C. 601 et seq.), that promulgation of
a SNUR would not have a significant
economic impact on a substantial
number of small entities where the
following are true:
1. A significant number of SNUNs
would not be submitted by small
entities in response to the SNUR.
2. The SNUN submitted by any small
entity would not cost significantly more
than $8,300.
A copy of that certification is
available in the docket for this proposed
rule.
This proposed rule is within the
scope of the February 18, 2012
certification. Based on the Economic
Analysis discussed in Unit IX. and
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EPA’s experience promulgating SNURs
(discussed in the certification), EPA
believes that the following are true:
• A significant number of SNUNs
would not be submitted by small
entities in response to the SNUR.
• Submission of the SNUN would not
cost any small entity significantly more
than $8,300. Therefore, the
promulgation of the SNUR would not
have a significant economic impact on
a substantial number of small entities.
D. Unfunded Mandates Reform Act
Based on EPA’s experience with
proposing and finalizing SNURs, State,
local, and Tribal governments have not
been impacted by these rulemakings,
and EPA does not have any reason to
believe that any State, local, or Tribal
government would be impacted by this
proposed rule. As such, EPA has
determined that this proposed rule
would not impose any enforceable duty,
contain any unfunded mandate, or
otherwise have any effect on small
governments subject to the requirements
of sections 202, 203, 204, or 205 of the
Unfunded Mandates Reform Act of 1995
(UMRA) (Pub. L. 104–4).
E. Executive Order 13132
This action would not have a
substantial direct effect on States, on the
relationship between the national
government and the States, or on the
distribution of power and
responsibilities among the various
levels of government, as specified in
Executive Order 13132, entitled
‘‘Federalism’’ (64 FR 43255, August 10,
1999).
F. Executive Order 13175
This proposed rule would not have
Tribal implications because it is not
expected to have substantial direct
effects on Indian Tribes. This proposed
rule would not significantly nor
uniquely affect the communities of
Indian Tribal governments, nor would it
involve or impose any requirements that
affect Indian Tribes. Accordingly, the
requirements of Executive Order 13175,
entitled ‘‘Consultation and Coordination
with Indian Tribal Governments’’ (65 FR
67249, November 9, 2000), do not apply
to this proposed rule.
G. Executive Order 13045
This action is not subject to Executive
Order 13045, entitled ‘‘Protection of
Children from Environmental Health
Risks and Safety Risks’’ (62 FR 19885,
April 23, 1997), because this is not an
economically significant regulatory
action as defined by Executive Order
12866, and this action does not address
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35335
environmental health or safety risks
disproportionately affecting children.
H. Executive Order 13211
This proposed rule is not subject to
Executive Order 13211, entitled
‘‘Actions Concerning Regulations That
Significantly Affect Energy Supply,
Distribution, or Use’’ (66 FR 28355, May
22, 2001), because this action is not
expected to affect energy supply,
distribution, or use and because this
action is not a significant regulatory
action under Executive Order 12866.
I. National Technology Transfer and
Advancement Act
In addition, since this action does not
involve any technical standards, section
12(d) of the National Technology
Transfer and Advancement Act of 1995
(NTTAA), Public Law 104–113, section
12(d) (15 U.S.C. 272 note), does not
apply to this action.
J. Executive Order 12898
This action does not entail special
considerations of environmental justice
related issues as delineated by
Executive Order 12898, entitled
‘‘Federal Actions to Address
Environmental Justice in Minority
Populations and Low-Income
Populations’’ (59 FR 7629, February 16,
1994).
List of Subjects in 40 CFR Part 725
Chemicals, Environmental protection,
Hazardous substances, Reporting and
recordkeeping requirements.
Dated: June 1, 2012.
Maria J. Doa,
Director, Chemical Control Division, Office
of Pollution Prevention and Toxics.
Therefore, it is proposed that 40 CFR
chapter I be amended as follows:
PART 725—[AMENDED]
1. The authority citation for part 725
continues to read as follows:
Authority: 15 U.S.C. 2604, 2607, 2613, and
2625.
2. Add § 725.1077 to subpart M to
read as follows:
§ 725.1077
Trichoderma reesei (generic).
(a) Microorganism and significant new
uses subject to reporting. (1) The
genetically modified microorganism
identified generically as Trichoderma
reesei (MCAN J–10–2) is subject to
reporting under this section for the
significant new uses described in
paragraph (a)(2) of this section.
(2)(i) The significant new use is any
manufacturing, processing, or use of the
microorganism other than in a
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Federal Register / Vol. 77, No. 114 / Wednesday, June 13, 2012 / Proposed Rules
fermentation system that meets all of the
following conditions:
(A) Submerged fermentation (i.e.,
growth of the microorganism occurs
beneath the surface of the liquid growth
medium).
(B) No solid plant material or
insoluble substrate is included with the
microorganism for fermentation.
(C) Any fermentation of solid plant
material or insoluble substrate, to which
fermentation broth is added, is initiated
only after the inactivation of the
microorganism as delineated in 40 CFR
725.422(d).
(ii) [Reserved]
(b) [Reserved]
[FR Doc. 2012–14242 Filed 6–12–12; 8:45 am]
BILLING CODE 6560–50–P
FEDERAL COMMUNICATIONS
COMMISSION
47 CFR Part 64
[CC Docket No. 96–115; DA 12–818]
Privacy and Security of Information
Stored on Mobile Communications
Devices
Federal Communications
Commission.
ACTION: Proposed rule.
AGENCY:
This document seeks
comment on the privacy and data
security practices of mobile wireless
services providers with respect to
customer information stored on their
users’ mobile communications devices.
In addition, the document seeks
comment on the application of existing
privacy and security requirements to
such information.
DATES: Comments may be filed on or
before July 13, 2012, and reply
comments may be filed on or before July
30, 2012.
ADDRESSES: You may submit comments,
identified by CC Docket No. 96–115, by
any of the following methods:
D Federal Communications
Commission’s Web Site: https://
fjallfoss.fcc.gov/ecfs2/. Follow the
instructions for submitting comments.
D Mail: See the SUPPLEMENTARY
INFORMATION section of this document.
D People with Disabilities: Contact the
FCC to request reasonable
accommodations (accessible format
documents, sign language interpreters,
CART, etc.) by email: FCC504@fcc.gov
or phone: 202–418–0530 or TTY: 202–
418–0432. For detailed instructions for
submitting comments and additional
information, see the SUPPLEMENTARY
INFORMATION section of this document.
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SUMMARY:
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For
further information regarding this
proceeding, contact Douglas Klein,
Office of General Counsel, (202) 418–
1720.
FOR FURTHER INFORMATION CONTACT:
This is a
summary of a Public Notice released by
the Wireline Competition Bureau, the
Wireless Telecommunications Bureau,
and the Office of General Counsel on
May 25, 2012. The full text of this
document is available for public
inspection and copying during regular
business hours in the Commission’s
Reference Information Center, Portals II,
445 12th Street SW., Room CY–A257,
Washington, DC 20554. The complete
text of this document also may be
purchased from the Commission’s copy
contractor, Best Copy and Printing, Inc.,
Portals II, 445 12th Street SW., Room
CY–B402, Washington, DC 20554,
telephone (202) 488–5300, facsimile
(202) 488–5563 or via email
FCC@BCPIWEB.com. The full text may
also be downloaded at https://
www.fcc.gov. Pursuant to §§ 1.415 and
1.419 of the Commission’s rules, 47 CFR
1.415, 1.419, interested parties may file
comments and reply comments on or
before the dates indicated on the first
page of this document. Comments may
be filed using the Commission’s
Electronic Comment Filing System
(ECFS). See Electronic Filing of
Documents in Rulemaking Proceedings,
63 FR 24121 (1998).
D Electronic Filers: Comments may be
filed electronically using the Internet by
accessing the ECFS: https://
fjallfoss.fcc.gov/ecfs2/.
D Paper Filers: Parties who choose to
file by paper must file an original and
one copy of each filing. If more than one
docket or rulemaking number appears in
the caption of this proceeding, filers
must submit two additional copies for
each additional docket or rulemaking
number.
Filings can be sent by hand or
messenger delivery, by commercial
overnight courier, or by first-class or
overnight U.S. Postal Service mail. All
filings must be addressed to the
Commission’s Secretary, Office of the
Secretary, Federal Communications
Commission.
D All hand-delivered or messengerdelivered paper filings for the
Commission’s Secretary must be
delivered to FCC Headquarters at 445
12th St. SW., Room TW–A325,
Washington, DC 20554. The filing hours
are 8:00 a.m. to 7:00 p.m. All hand
deliveries must be held together with
rubber bands or fasteners. Any
envelopes and boxes must be disposed
of before entering the building.
SUPPLEMENTARY INFORMATION:
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Fmt 4702
Sfmt 4702
D Commercial overnight mail (other
than U.S. Postal Service Express Mail
and Priority Mail) must be sent to 9300
East Hampton Drive, Capitol Heights,
MD 20743.
D U.S. Postal Service first-class,
Express, and Priority mail must be
addressed to 445 12th Street SW.,
Washington, DC 20554.
Documents will be available for
public inspection and copying during
business hours at the FCC Reference
Information Center, Portals II, Room
CY–A257, 445 12th Street SW.,
Washington, DC 20554. The documents
may also be purchased from BCPI,
telephone (202) 488–5300, facsimile
(202) 488–5563, TTY (202) 488–5562,
email fcc@bcpiweb.com.
People with Disabilities: To request
materials in accessible formats for
people with disabilities (Braille, large
print, electronic files, audio format),
send an email to fcc504@fcc.gov or call
the Consumer & Governmental Affairs
Bureau at 202–418–0530 (voice), 202–
418–0432 (tty).
The Commission has designated this
proceeding as a ‘‘permit-but-disclose’’
proceeding in accordance with the
Commission’s ex parte rules. 47 CFR
1.1200 et seq.; Amendment of Certain of
the Commission’s Part 1 Rules of
Practice and Procedure and Part 0 Rules
of Commission Organization, Notice of
Proposed Rulemaking, 25 FCC Rcd
2430, 2439–40 (2010). Persons making
ex parte presentations must file a copy
of any written presentation or a
memorandum summarizing any oral
presentation within two business days
after the presentation (unless a different
deadline applicable to the Sunshine
period applies). Persons making oral ex
parte presentations are reminded that
memoranda summarizing the
presentation must (1) list all persons
attending or otherwise participating in
the meeting at which the ex parte
presentation was made, and (2)
summarize all data presented and
arguments made during the
presentation. If the presentation
consisted in whole or in part of the
presentation of data or arguments
already reflected in the presenter’s
written comments, memoranda or other
filings in the proceeding, the presenter
may provide citations to such data or
arguments in his or her prior comments,
memoranda, or other filings (specifying
the relevant page and/or paragraph
numbers where such data or arguments
can be found) in lieu of summarizing
them in the memorandum. Documents
shown or given to Commission staff
during ex parte meetings are deemed to
be written ex parte presentations and
must be filed consistent with § 1.1206(b)
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Agencies
[Federal Register Volume 77, Number 114 (Wednesday, June 13, 2012)]
[Proposed Rules]
[Pages 35331-35336]
From the Federal Register Online via the Government Printing Office [www.gpo.gov]
[FR Doc No: 2012-14242]
-----------------------------------------------------------------------
ENVIRONMENTAL PROTECTION AGENCY
40 CFR Part 725
[EPA-HQ-OPPT-2010-0994; FRL-9350-6]
RIN 2070-AD43
Trichoderma reesei; Proposed Significant New Use Rule
AGENCY: Environmental Protection Agency (EPA).
ACTION: Proposed rule.
-----------------------------------------------------------------------
SUMMARY: EPA is proposing a significant new use rule (SNUR) under the
Toxic Substances Control Act (TSCA) for the genetically modified
microorganism identified generically as Trichoderma reesei (T. reesei).
This microorganism was the subject of a Microbial Commercial Activity
Notice (MCAN). EPA believes this action is necessary because the use of
this genetically modified T. reesei under certain conditions may be
hazardous to human health and the environment. This proposed rule would
also establish a mechanism to allow EPA to evaluate an intended use and
its conditions, and to prohibit or limit that activity before it
occurs, if EPA determines it may be hazardous.
DATES: Comments must be received on or before July 13, 2012.
ADDRESSES: Submit your comments, identified by docket identification
(ID) number EPA-HQ-OPPT-2010-0994, by one of the following methods:
Federal eRulemaking Portal: https://www.regulations.gov.
Follow the online instructions for submitting comments.
Mail: Document Control Office (7407M), Office of Pollution
Prevention and Toxics (OPPT), Environmental Protection Agency, 1200
Pennsylvania Ave. NW., Washington, DC 20460-0001.
Hand Delivery: OPPT Document Control Office (DCO), EPA
East Bldg., Rm. 6428, 1201 Constitution Ave. NW., Washington, DC.
Attention: Docket ID Number EPA-HQ-OPPT-2010-0994. The DCO is open from
8 a.m. to 4 p.m., Monday through Friday, excluding legal holidays. The
telephone number for the DCO is (202) 564-8930. Such deliveries are
only accepted during the DCO's normal hours of operation, and special
arrangements should be made for deliveries of boxed information.
Instructions: Direct your comments to docket ID number EPA-HQ-OPPT-
2010-0994. EPA's policy is that all comments received will be included
in the docket without change and may be made available online at https://www.regulations.gov, including any personal information provided,
unless the comment includes information claimed to be Confidential
Business Information (CBI) or other information whose disclosure is
restricted by statute. Do not submit information that you consider to
be CBI or otherwise protected through regulations.gov or email. The
regulations.gov Web site is an ``anonymous access'' system, which means
EPA will not know your identity or contact information unless you
provide it in the body of your comment. If you send an email comment
directly to EPA without going through regulations.gov, your email
address will be automatically captured and included as part of the
comment that is placed in the docket and made available on the
Internet. If you submit an electronic comment, EPA recommends that you
include your name and other contact information in the body of your
comment and with any disk or CD-ROM you submit. If EPA cannot read your
comment due to technical difficulties and cannot contact you for
clarification, EPA may not be able to consider your comment. Electronic
files should avoid the use of special characters, any form of
encryption, and be free of any defects or viruses.
Docket: All documents in the docket are listed in the docket index
available at https://www.regulations.gov. Although listed in the index,
some information is not publicly available, e.g., CBI or other
information whose disclosure is restricted by statute. Certain other
material, such as copyrighted material, will be publicly available only
in hard copy. Publicly available docket materials are available
electronically at https://www.regulations.gov, or, if only available in
hard copy, at the OPPT Docket. The OPPT Docket is located in the EPA
Docket Center (EPA/DC) at Rm. 3334, EPA West Bldg., 1301 Constitution
Ave. NW., Washington, DC. The EPA/DC Public Reading Room hours of
operation are 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding
legal holidays. The telephone number of the EPA/DC Public Reading Room
is (202) 566-1744, and the telephone number for the OPPT Docket is
(202) 566-0280. Docket visitors are required to show photographic
identification, pass through a metal detector, and sign the EPA visitor
log. All visitor bags are processed through an X-ray machine and
subject to search. Visitors will be provided an EPA/DC badge that must
be visible at all times in the building and returned upon departure.
FOR FURTHER INFORMATION CONTACT: For technical information contact:
Kenneth Moss, Chemical Control Division (7405M), Office of Pollution
Prevention and Toxics, Environmental Protection Agency, 1200
Pennsylvania Ave. NW., Washington, DC 20460-0001; telephone number:
(202) 564-9232; email address: moss.kenneth@epa.gov.
For general information contact: The TSCA-Hotline, ABVI-Goodwill,
422 South Clinton Ave., Rochester, NY 14620; telephone number: (202)
554-1404; email address: TSCA-Hotline@epa.gov.
SUPPLEMENTARY INFORMATION:
[[Page 35332]]
I. General Information
A. Does this action apply to me?
You may be potentially affected by this action if you manufacture,
import, process, or use products that contain living microorganisms
subject to TSCA, especially if you know that your products contain or
may contain T. reesei. Potentially affected entities may include, but
are not limited to:
Manufacturers, importers, or processors of chemical
substances (NAICS codes 325 and 324110), e.g., chemical manufacturing
and petroleum refineries.
This listing is not intended to be exhaustive, but rather provides
a guide for readers regarding entities likely to be affected by this
action. Other types of entities not listed in this unit could also be
affected. The North American Industrial Classification System (NAICS)
codes have been provided to assist you and others in determining
whether this action might apply to certain entities. To determine
whether you or your business may be affected by this action, you should
carefully examine the list of chemical substances excluded by TSCA
section 3(2)(B) and the applicability provisions in Sec. 725.105(c)
for SNUR related obligations. If you have any questions regarding the
applicability of this action to a particular entity, consult the
technical person listed under FOR FURTHER INFORMATION CONTACT.
This action may also affect certain entities through pre-existing
import certification and export notification rules under TSCA. Chemical
importers are subject to the TSCA section 13 (15 U.S.C. 2612) import
certification requirements promulgated at 19 CFR 12.118 through 12.127;
see also 19 CFR 127.28. Chemical importers must certify that the
shipment of the chemical substance complies with all applicable rules
and orders under TSCA. Importers of chemical substances subject to
these SNURs must certify their compliance with the SNUR requirements.
The EPA policy in support of import certification appears at 40 CFR
part 707, subpart B. In addition, any persons who export or intend to
export a chemical substance that is the subject of a proposed or final
SNUR are subject to the export notification provisions of TSCA section
12(b) (15 U.S.C. 2611(b)) (see Sec. [emsp14]725.920), and must comply
with the export notification requirements in 40 CFR part 707, subpart
D.
B. What should I consider as I prepare my comments for EPA?
1. Submitting CBI. Do not submit this information to EPA through
regulations.gov or email. Clearly mark the part or all of the
information that you claim to be CBI. For CBI information in a disk or
CD-ROM that you mail to EPA, mark the outside of the disk or CD-ROM as
CBI and then identify electronically within the disk or CD-ROM the
specific information that is claimed as CBI. In addition to one
complete version of the comment that includes information claimed as
CBI, a copy of the comment that does not contain the information
claimed as CBI must be submitted for inclusion in the public docket.
Information so marked will not be disclosed except in accordance with
procedures set forth in 40 CFR part 2.
2. Tips for preparing your comments. When submitting comments,
remember to:
i. Identify the document by docket ID number and other identifying
information (subject heading, Federal Register date and page number).
ii. Follow directions. The Agency may ask you to respond to
specific questions or organize comments by referencing a Code of
Federal Regulations (CFR) part or section number.
iii. Explain why you agree or disagree; suggest alternatives and
substitute language for your requested changes.
iv. Describe any assumptions and provide any technical information
and/or data that you used.
v. If you estimate potential costs or burdens, explain how you
arrived at your estimate in sufficient detail to allow for it to be
reproduced.
vi. Provide specific examples to illustrate your concerns and
suggest alternatives.
vii. Explain your views as clearly as possible, avoiding the use of
profanity or personal threats.
viii. Make sure to submit your comments by the comment period
deadline identified.
II. Background
A. What action is the agency taking?
EPA is proposing this SNUR for the genetically modified
microorganism identified generically as T. reesei (MCAN J-10-2). This
proposed rule would require persons to notify EPA at least 90 days
before commencing the manufacture, import, or processing of the
microorganism for any activity designated as a significant new use.
B. What is the agency's authority for taking this action?
Section 5(a)(2) of TSCA (15 U.S.C. 2604(a)(2)) authorizes EPA to
determine that a use of a chemical substance is a ``significant new
use.'' (see 40 CFR part 725, subparts L and M). EPA must make this
determination by rule after considering all relevant factors, including
the TSCA section 5(a)(2) factors, listed in Unit III. Once EPA
determines that a use of a chemical substance is a significant new use,
TSCA section 5(a)(1)(B) requires persons to submit a significant new
use notice (SNUN) to EPA at least 90 days before they manufacture,
import, or process the chemical substance for that use. Persons who
must report are described in Sec. 725.105(c).
EPA has interpreted the TSCA section 3(2) definition of ``chemical
substance'' as authorizing EPA to regulate microorganisms under TSCA.
See the Federal Register issue of April 11, 1997 (62 FR 17910) (FRL-
5577-2).
C. Applicability of General Provisions
General provisions for SNURs for microorganisms appear in 40 CFR
part 725, subpart L. These provisions describe persons subject to the
proposed rule, recordkeeping requirements, exemptions to reporting
requirements, and applicability to uses occurring before the effective
date of the final rule. Provisions relating to user fees appear at 40
CFR part 700. Persons subject to this SNUR must comply with the notice
requirements under TSCA section 5(a)(1)(A) and must submit a MCAN,
using the procedures set out in 40 CFR part 725, subpart D, and
additional ``Significant New Uses of Microorganisms'' procedures at 40
CFR part 725, subpart L.
Under 40 CFR part 725, EPA has adopted a more narrow interpretation
of the TSCA section 5(h)(3) exemption for small quantities used in
research than it has for other chemical substances under 40 CFR part
721. Under Sec. 725.3, EPA has defined small quantities solely for
research and development as ``quantities of a microorganism
manufactured, imported, or processed or proposed to be manufactured,
imported, or processed solely for research and development that meet
the requirements of Sec. 725.234.'' Any other research and development
activity of a microorganism subject to a SNUR must comply with the TSCA
section 5(a)(1)(A) notification requirements unless that activity has
been excluded from coverage under the SNUR. See Sec. 725.3, subparts E
and F of 40 CFR part 725, and the April 11, 1997 Federal Register
document. Once EPA receives a SNUN, EPA may take regulatory action
under TSCA section 5(e), 5(f), 6, or 7 to control the activities for
which it has received the SNUN. If EPA does not take action, EPA is
required under TSCA section 5(g) to explain in the
[[Page 35333]]
Federal Register its reasons for not taking action.
III. Significant New Use Determination
Section 5(a)(2) of TSCA states that EPA's determination that a use
of a chemical substance is a significant new use must be made after
consideration of all relevant factors, including:
The projected volume of manufacturing and processing of a
chemical substance.
The extent to which a use changes the type or form of
exposure of human beings or the environment to a chemical substance.
The extent to which a use increases the magnitude and
duration of exposure of human beings or the environment to a chemical
substance.
The reasonably anticipated manner and methods of
manufacturing, processing, distribution in commerce, and disposal of a
chemical substance.
In addition to these factors specifically enumerated in TSCA section
5(a)(2), the statute authorizes EPA to consider any other relevant
factors.
To determine what would constitute a significant new use for the
chemical substance that is the subject of this proposed SNUR, EPA
considered the available information relating to the four bulleted
factors listed in TSCA section 5(a)(2) factors listed in this unit, and
other relevant factors. This includes relevant information about the
toxicity of the chemical substance and likely human exposures and
environmental releases associated with possible uses. See the risk
assessment in the docket under docket ID number EPA-HQ-OPPT-2010-0994
for this information and other relevant factors.
IV. Substance Subject to This Proposed Rule
EPA is proposing to establish significant new use and recordkeeping
requirements for only the microorganism identified generically as T.
reesei, genetically modified as described in MCAN J-10-2. This will be
codified in 40 CFR part 725, subpart M. Any T. reesei microorganism
with genetic modifications other than those described in MCAN J-10-2
would not be subject to this SNUR and will require submission and EPA
review of a separate MCAN.
MCAN Number J-10-2
Chemical name: Trichoderma reesei (MCAN J-10-2) (generic).
Chemical Abstracts Service (CAS) Registry Number: Not available.
Use: The MCAN states that the generic (non-confidential) use of the
microorganism will be to produce enzymes for ethanol production.
Basis for action: When used to produce enzymes that can release
sugars from de-lignified plant materials, human and environmental
exposures to live T. reesei cells are low, due to the containment and
inactivation procedures specified in the MCAN. These containment and
inactivation procedures are consistent with standard industry practices
and those delineated in 40 CFR 725.422(d). These procedures include the
use of equipment to minimize aerosol releases from the facility, and
the use of inactivation methods that reduce the number of viable cells
by at least 6 logs (i.e., 10\6\) in the liquid and solid waste streams.
More importantly, the manufacturing process described in the MCAN
relies on the typical submerged standard industrial fermentation
process for enzyme production, wherein the microorganism is grown in
liquid broth culture in the absence of solid materials or solid
surfaces, the fermentation is terminated prior to the microorganism
entering the stationary phase of growth, and the enzyme is separated
from the microbial biomass which is inactivated prior to disposal.
Therefore, EPA determined that the proposed manufacturing, processing,
or use of the microorganism as described in the MCAN is not expected to
present an unreasonable risk. However, EPA has determined that use of
the microorganism under other conditions may result in adverse human
health and environmental effects. Specifically, where growth on solid
plant material or insoluble substrate occurs, T. reesei has been shown
to produce a secondary metabolite known as paracelsin, which is a
peptaibol. Peptaibols are small linear peptides of 1,000-2,000 Daltons
characterized by a high content of the non-proteinogenic amino acid
alpha-amino-isobutyric acid (Aib), with a N-terminus that is typically
acetylated, and a C-terminus that is linked to an amino alcohol, which
is usually phenylalaninol, or sometimes valinol, leucinol, isoleucinol,
or tryptophanol. Peptaibols are associated with a wide variety of
biological activities and have antifungal, antibacterial, sometimes
antiviral, antiparasitic, and neurotoxic activity. Paracelsin has been
shown to have toxicity toward mammalian cells such as hemolytic
activity on human erythrocytes and cytotoxicity to rat adrenal medulla
PC12 (pheochromocytoma) cells. Paracelsin has also been shown to
exhibit cytotoxicity to Gram-positive bacteria, to human erythrocytes,
and to other mammals such as aquatic indicator species. Additional
information relating to the assessment of this chemical substance and
paracelsin, including a sanitized EPA risk assessment and a list of
references used, is available in the docket under docket ID number EPA-
HQ-OPPT-2010-0994.
Recommended testing: EPA has determined that the results of the
following studies would help characterize any potential human health
and environmental effects of the MCAN substance:
1. Investigation of whether paracelsin will be produced, and at
what levels if the genetically modified T. reesei is grown on various
plant biomass materials for different durations under various
fermentation conditions in cellulosic biomass facilities.
2. If paracelsin is produced, a study of whether paracelsin would
be denatured/inactivated during production and processing.
3. If paracelsin is released from the facility, a study of whether
paracelsin would be degraded/inactivated during wastewater treatment.
4. If released to the environment, studies on the persistence,
stability, dissemination, accumulation, and the potential resulting
biological activity of paracelsin with exposure to aquatic and
terrestrial organisms in the environment.
5. Studies to determine the ability of the MCAN microorganism to
survive in the environment relative to the survival of the unmodified
parent or recipient strain, and to assess its competitiveness with
other fungi in the environment. This study may require some
supplementation with one or more carbon sources and the use of various
soil types.
6. A study to determine survival of the fungus during an anaerobic
fermentation for production of ethanol by an ethanologen, and survival
of the fungus during ethanol distillation or at the distillation
temperature for ethanol.
CFR citation: 40 CFR 725.1077.
V. Rationale and Objectives of the Proposed Rule
A. Rationale
During review of the specific T. reesei, modified as described in
MCAN J-10-2, EPA determined that certain fermentation conditions, other
than the typical submerged standard industrial fermentation process for
enzyme production described in Unit IV., could result in increased
exposures thereby constituting a ``significant new use.'' Specifically,
EPA is concerned that where growth on solid plant material or
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insoluble substrate occurs, T. reesei has been shown to produce a
secondary metabolite known as paracelsin, which is associated with a
variety of toxic effects to mammalian and bacterial cells. Use of the
MCAN microorganism without the specific containment or inactivation
controls listed in the MCAN, described in Unit IV., may result in
adverse human health and environmental effects. Based on the
descriptions of manufacturing, processing, and use in the MCAN J-10-2,
the Agency believes that uses of the organism covered by the proposed
definition of a significant new use are not currently ongoing.
B. Objectives
EPA is proposing this SNUR for a chemical substance that has
undergone review to achieve the following objectives with regard to the
significant new uses designated in this proposed rule:
EPA would receive notice of any person's intent to
manufacture, import, or process a listed chemical substance for the
described significant new use before that activity begins.
EPA would have an opportunity to review and evaluate data
submitted in a SNUN before the notice submitter begins manufacturing,
importing, or processing a listed chemical substance for the described
significant new use.
EPA would be able to determine whether regulation of
prospective manufacturers, importers, or processors of a listed
chemical substance is warranted pursuant to TSCA sections 5(e), 5(f),
6, or 7, and impose any necessary requirements before the described
significant new use of that chemical substance occurs.
Issuance of a SNUR for a chemical substance does not signify that
the chemical substance is listed on the TSCA Chemical Substance
Inventory (TSCA Inventory). Guidance on how to determine if a chemical
substance is on the TSCA Inventory is available electronically at
https://www.epa.gov/opptintr/existingchemicals/pubs/tscainventory/.
VI. Applicability of Proposed Rule to Uses Occurring Before Effective
Date of the Final Rule
To establish a significant ``new'' use, EPA must determine that the
use is not ongoing. EPA solicits comments on whether any of the uses
proposed as significant new uses are ongoing.
As discussed in the Federal Register issue of April 24, 1990 (55 FR
17376), EPA has decided that the intent of TSCA section 5(a)(1)(B) is
best served by designating a use as a significant new use as of the
date of publication of the proposed rule rather than as of the
effective date of the final rule. If uses begun after publication of
the proposed rule were considered ongoing rather than new, it would be
difficult for EPA to establish SNUR notice requirements because a
person could defeat the SNUR by initiating the significant new use
before the proposed rule became final, and then argue that the use was
ongoing before the effective date of the final rule. Thus, any persons
who begin commercial manufacture, import, or processing activities with
the microorganism that would be regulated through this proposed rule
will have to cease any such activity before the effective date of the
final rule, if and when finalized. To resume their activities, these
persons would have to comply with all applicable SNUR notice
requirements and wait until the notice review period, including all
extensions, expires.
EPA has promulgated provisions to allow persons to comply with this
proposed SNUR before the effective date. If a person were to meet the
conditions of advance compliance under 40 CFR 725.912(a), the person
would be considered exempt from the requirements of the SNUR.
VII. Test Data and Other Information
EPA recognizes that TSCA section 5 does not require developing any
particular test data before submission of a SNUN. There are two
exceptions:
1. Development of test data is required where the chemical
substance subject to the SNUR is also subject to a test rule under TSCA
section 4 (see TSCA section 5(b)(1)).
2. Development of test data may be necessary where the chemical
substance has been listed under TSCA section 5(b)(4) (see TSCA section
5(b)(2)).
In the absence of a TSCA section 4 test rule or a TSCA section
5(b)(4) listing covering the chemical substance, persons are required
only to submit test data in their possession or control and to describe
any other data known to or reasonably ascertainable by them (see 40 CFR
725.25(a)(2)). However, upon review of MCANs and SNUNs, the Agency has
the authority to require appropriate testing. In this case, EPA
recommends persons, before performing any testing, to consult with the
Agency pertaining to protocol selection.
The recommended testing specified in Unit IV. may not be the only
means of addressing the potential risks for the chemical substance.
However, SNUNs submitted without any test data may increase the
likelihood that EPA will respond by taking action under TSCA section
5(e), particularly if satisfactory test results have not been obtained
from a prior submission. EPA recommends that potential SNUN submitters
contact EPA early enough so that they will be able to conduct the
appropriate tests.
SNUN submitters should be aware that EPA will be better able to
evaluate SNUNs which provide detailed information on the following:
Human exposure and environmental release that may result
from the significant new use of the chemical substance.
Potential benefits of the chemical substance.
Information on risks posed by the chemical substance
compared to risks posed by potential substitutes.
VIII. SNUN Submissions
Persons subject to this SNUR must comply with the notice
requirements under TSCA section 5(a)(1)(A) and must submit a MCAN,
using the procedures set out in 40 CFR part 725, subpart D, and
additional ``Significant New Uses of Microorganisms'' procedures at 40
CFR part 725, subpart L. SNUNs must be submitted to EPA on EPA Form No.
6300-07, generated using e-PMN software, and submitted to the Agency in
accordance with the procedures set forth in 40 CFR 725.25 and 40 CFR
725.27. E-PMN software is available electronically at https://www.epa.gov/opptintr/newchems.
IX. Economic Analysis
EPA has evaluated the potential costs of establishing SNUN
requirements for potential manufacturers, importers, and processors of
the chemical substance subject to this proposed rule. EPA's complete
Economic Analysis is available in the docket under docket ID number
EPA-HQ-OPPT-2010-0994.
X. Statutory and Executive Order Reviews
A. Executive Order 12866
This proposed rule would establish a SNUR for a chemical substance
that was the subject of a MCAN. The Office of Management and Budget
(OMB) has exempted these types of actions from review under Executive
Order 12866, entitled ``Regulatory Planning and Review'' (58 FR 51735,
October 4, 1993).
B. Paperwork Reduction Act
According to the Paperwork Reduction Act (PRA) (44 U.S.C. 3501 et
seq.), an agency may not conduct or sponsor, and a person is not
required to respond to a collection of information that requires OMB
approval under PRA, unless it has been approved by OMB and displays a
currently valid OMB
[[Page 35335]]
control number. The OMB control numbers for EPA's regulations in title
40 of the CFR, after appearing in the Federal Register, are listed in
40 CFR part 9, and included on the related collection instrument or
form, if applicable. EPA would amend the table in 40 CFR part 9 to list
the OMB approval number for the information collection requirements
contained in this proposed rule. This listing of the OMB control
numbers and their subsequent codification in the CFR satisfies the
display requirements of PRA and OMB's implementing regulations at 5 CFR
part 1320.
The information collection requirements related to this action have
already been approved by OMB pursuant to PRA under OMB control number
2070-0012 (EPA ICR No. 574). This action would not impose any burden
requiring additional OMB approval. If an entity were to submit a SNUN
to the Agency, the annual burden is estimated to average between 30 and
170 hours per response. This burden estimate includes the time needed
to review instructions, search existing data sources, gather and
maintain the data needed, and complete, review, and submit the required
SNUN.
Send any comments about the accuracy of the burden estimate, and
any suggested methods for minimizing respondent burden, including
through the use of automated collection techniques, to the Director,
Collection Strategies Division, Office of Environmental Information
(2822T), Environmental Protection Agency, 1200 Pennsylvania Ave. NW.,
Washington, DC 20460-0001. Please remember to include the OMB control
number in any correspondence, but do not submit any completed forms to
this address.
C. Regulatory Flexibility Act
On February 18, 2012, EPA certified pursuant to section 605(b) of
the Regulatory Flexibility Act (RFA) (5 U.S.C. 601 et seq.), that
promulgation of a SNUR would not have a significant economic impact on
a substantial number of small entities where the following are true:
1. A significant number of SNUNs would not be submitted by small
entities in response to the SNUR.
2. The SNUN submitted by any small entity would not cost
significantly more than $8,300.
A copy of that certification is available in the docket for this
proposed rule.
This proposed rule is within the scope of the February 18, 2012
certification. Based on the Economic Analysis discussed in Unit IX. and
EPA's experience promulgating SNURs (discussed in the certification),
EPA believes that the following are true:
A significant number of SNUNs would not be submitted by
small entities in response to the SNUR.
Submission of the SNUN would not cost any small entity
significantly more than $8,300. Therefore, the promulgation of the SNUR
would not have a significant economic impact on a substantial number of
small entities.
D. Unfunded Mandates Reform Act
Based on EPA's experience with proposing and finalizing SNURs,
State, local, and Tribal governments have not been impacted by these
rulemakings, and EPA does not have any reason to believe that any
State, local, or Tribal government would be impacted by this proposed
rule. As such, EPA has determined that this proposed rule would not
impose any enforceable duty, contain any unfunded mandate, or otherwise
have any effect on small governments subject to the requirements of
sections 202, 203, 204, or 205 of the Unfunded Mandates Reform Act of
1995 (UMRA) (Pub. L. 104-4).
E. Executive Order 13132
This action would not have a substantial direct effect on States,
on the relationship between the national government and the States, or
on the distribution of power and responsibilities among the various
levels of government, as specified in Executive Order 13132, entitled
``Federalism'' (64 FR 43255, August 10, 1999).
F. Executive Order 13175
This proposed rule would not have Tribal implications because it is
not expected to have substantial direct effects on Indian Tribes. This
proposed rule would not significantly nor uniquely affect the
communities of Indian Tribal governments, nor would it involve or
impose any requirements that affect Indian Tribes. Accordingly, the
requirements of Executive Order 13175, entitled ``Consultation and
Coordination with Indian Tribal Governments'' (65 FR 67249, November 9,
2000), do not apply to this proposed rule.
G. Executive Order 13045
This action is not subject to Executive Order 13045, entitled
``Protection of Children from Environmental Health Risks and Safety
Risks'' (62 FR 19885, April 23, 1997), because this is not an
economically significant regulatory action as defined by Executive
Order 12866, and this action does not address environmental health or
safety risks disproportionately affecting children.
H. Executive Order 13211
This proposed rule is not subject to Executive Order 13211,
entitled ``Actions Concerning Regulations That Significantly Affect
Energy Supply, Distribution, or Use'' (66 FR 28355, May 22, 2001),
because this action is not expected to affect energy supply,
distribution, or use and because this action is not a significant
regulatory action under Executive Order 12866.
I. National Technology Transfer and Advancement Act
In addition, since this action does not involve any technical
standards, section 12(d) of the National Technology Transfer and
Advancement Act of 1995 (NTTAA), Public Law 104-113, section 12(d) (15
U.S.C. 272 note), does not apply to this action.
J. Executive Order 12898
This action does not entail special considerations of environmental
justice related issues as delineated by Executive Order 12898, entitled
``Federal Actions to Address Environmental Justice in Minority
Populations and Low-Income Populations'' (59 FR 7629, February 16,
1994).
List of Subjects in 40 CFR Part 725
Chemicals, Environmental protection, Hazardous substances,
Reporting and recordkeeping requirements.
Dated: June 1, 2012.
Maria J. Doa,
Director, Chemical Control Division, Office of Pollution Prevention and
Toxics.
Therefore, it is proposed that 40 CFR chapter I be amended as
follows:
PART 725--[AMENDED]
1. The authority citation for part 725 continues to read as
follows:
Authority: 15 U.S.C. 2604, 2607, 2613, and 2625.
2. Add Sec. 725.1077 to subpart M to read as follows:
Sec. 725.1077 Trichoderma reesei (generic).
(a) Microorganism and significant new uses subject to reporting.
(1) The genetically modified microorganism identified generically as
Trichoderma reesei (MCAN J-10-2) is subject to reporting under this
section for the significant new uses described in paragraph (a)(2) of
this section.
(2)(i) The significant new use is any manufacturing, processing, or
use of the microorganism other than in a
[[Page 35336]]
fermentation system that meets all of the following conditions:
(A) Submerged fermentation (i.e., growth of the microorganism
occurs beneath the surface of the liquid growth medium).
(B) No solid plant material or insoluble substrate is included with
the microorganism for fermentation.
(C) Any fermentation of solid plant material or insoluble
substrate, to which fermentation broth is added, is initiated only
after the inactivation of the microorganism as delineated in 40 CFR
725.422(d).
(ii) [Reserved]
(b) [Reserved]
[FR Doc. 2012-14242 Filed 6-12-12; 8:45 am]
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