Government-Owned Inventions; Availability for Licensing, 53265-53267 [E9-24871]
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Federal Register / Vol. 74, No. 199 / Friday, October 16, 2009 / Notices
developing a mouse model for studying
the effect of down-regulating these
receptors specifically in melanoma
cells. This would mimic the effect of
antagonists without the confounding
effects of systemically inhibiting CXCR4
or CCR10. By either adding or removing
dietary administered doxycycline,
receptor expression can be regulated to
assess the role of these two receptors in
a variety of cancer-related assays.
Applications:
• Study the effect of chemokine
receptors in tumor growth or metastasis.
• Test CXCR4 and CCR10 antagonists
in preclinical studies.
• Develop B16 melanoma mouse
model mimicking the effect of
chemokine receptor antagonists.
Advantages:
• Ability to regulate in vitro and in
vivo expression of the chemokine
receptor.
• Ability to investigate the in vivo
role in cancer cells of doxycycline
control of chemokine receptor
expression.
Development Status: The technology
is currently in the preclinical stage of
development.
Market: Cancer is the second leading
cause of death in the U.S. and it is
estimated that more than 1 million
Americans develop cancer in a year.
Inventors: Sam T. Hwang (NCI).
Publication: T Kakinuma, ST Hwang.
Chemokines, chemokine receptors, and
cancer metastasis. J Leukoc Biol. 2006
Apr;79(4):639–651.
Patent Status: HHS Reference No. E–
345–2008/0—Research Material. Patent
protection is not being sought for either
technology.
Licensing Status: Available for nonexclusive licensing under a Biological
Materials License Agreement.
Licensing Contact: Betty B. Tong,
Ph.D.; 301–594–6565;
tongb@mail.nih.gov.
jlentini on DSKJ8SOYB1PROD with NOTICES
Identification of Persons Likely To
Benefit From Statin Mediated Cancer
Prevention by Pharmacogenetics
Description of Technology: Inhibitors
of 3-hydroxy-3-methylglutaryl (HMG)
coenzyme A reductase (statins) are a
class of well-tolerated compounds that
are the most widely used cholesterollowering drugs in the United States.
Reduced cancer risk among statin users
has also been observed as a secondary
outcome in randomized controlled
clinical trials evaluating effects of
statins on cardiovascular outcomes.
However the observed cancer risk
reduction varied with different clinical
studies. Thus there is a need to identify
individuals who would benefit from
treatment with statins.
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The current invention describes a
pharmacogenetic method to identify
candidates who are most likely to
benefit from treatment with statins to
reduce cancer risk, and consequently
minimizing any unnecessary cost and
side effects in individuals who do not
benefit. Specifically, we discovered that
an HMGCR genetic variant rs12654264
is associated with significantly lower
colorectal cancer risk, with most of the
benefit seen in HMGCoA reductase
inhibitor (statin) users. We also
discovered that this same HMGCR
genetic variant is associated with
significantly higher serum cholesterol
levels in Israeli colorectal cancer
patients. The same HMGCR genetic
variant has also been associated with
significantly higher serum cholesterol
levels in two independent groups of
individuals of mixed European descent
[https://www.broad.mit.edu/diabetes/
scandinavs/ and N Engl J
Med.2008 March 20;358(12):1240–1249
(https://www.ncbi.nlm.nih.gov/pubmed/
18354102?dopt)]. These data suggest
that the same genetic variant modifies
cholesterol metabolism in a manner that
affects both colorectal cancer risk and
cardiovascular risk.
Applications and Market:
• Statins account for approximately
80% of the cholesterol-lowering drugs
prescribed in the United States, and six
statins are currently available on the
U.S. market. Reduced cancer risk is also
associated with statin use. This
invention provides a method to
indentify individuals who are most
likely to benefit from cancer
chemopreventive treatment with statins.
• Pharmacogenetic markers can be
developed to identify patient population
that can be benefit from statins,
therefore expands the markets of stains.
Development Status: The inventors
have discovered several novel genetic
variants of HMG coenzyme A reductase
gene, and are further investigating the
functional significance of the variants in
vitro.
Inventors: Levy Kopelovich (NCI) et
al.
Patent Status: PCT Application No.
PCT/US2008/082359 filed 04 Nov 2008,
which published as WO 2009/061734
on 14 May 2009 (HHS Reference No. E–
328–2007/0–PCT–02).
Licensing Status: Available for
licensing.
Licensing Contact: Betty B. Tong,
Ph.D.; 301–594–6565;
tongb@mail.nih.gov.
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53265
Dated: October 7, 2009.
Richard U. Rodriguez,
Director, Division of Technology Development
and Transfer, Office of Technology Transfer,
National Institutes of Health.
[FR Doc. E9–24877 Filed 10–15–09; 8:45 am]
BILLING CODE 4140–01–P
DEPARTMENT OF HEALTH AND
HUMAN SERVICES
National Institutes of Health
Government-Owned Inventions;
Availability for Licensing
AGENCY: National Institutes of Health,
Public Health Service, HHS.
ACTION: Notice.
SUMMARY: The inventions listed below
are owned by an agency of the U.S.
Government and are available for
licensing in the U.S. in accordance with
35 U.S.C. 207 to achieve expeditious
commercialization of results of
federally-funded research and
development. Foreign patent
applications are filed on selected
inventions to extend market coverage
for companies and may also be available
for licensing.
ADDRESSES: Licensing information and
copies of the U.S. patent applications
listed below may be obtained by writing
to the indicated licensing contact at the
Office of Technology Transfer, National
Institutes of Health, 6011 Executive
Boulevard, Suite 325, Rockville,
Maryland 20852–3804; telephone: 301/
496–7057; fax: 301/402–0220. A signed
Confidential Disclosure Agreement will
be required to receive copies of the
patent applications.
Biological/Research Material for H1N1
Influenza Virus Vaccine Research
Description of Technology: Offered for
licensing is a recombinant attenuated
vaccinia virus, MVA, that expresses the
haemagglutinin (HA) and nucleoprotein
(NP) of influenza virus A/PR/8/34
(H1N1). The virus has been shown to
stimulate protective immunity to
influenza virus in mice.
The materials can be used for research
purposes and in particular in the area of
influenza virus vaccines.
The related publications listed below
demonstrate the usefulness of this
biological material in influenza virus
vaccine research.
Applications: Research reagents
useful in research and development in
the area of H1N1 Influenza virus
vaccines.
Development Status: Fully developed.
The usefulness of the materials has been
shown in Dr. Moss’ laboratory.
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53266
Federal Register / Vol. 74, No. 199 / Friday, October 16, 2009 / Notices
jlentini on DSKJ8SOYB1PROD with NOTICES
Inventors: Bernard Moss and Linda S.
Wyatt (NIAID).
Publications:
1. G Sutter, LS Wyatt, PL Foley, JR
Bennink, B Moss. A recombinant vector
derived from the host range-restricted
and highly attenuated MVA strain of
vaccinia virus stimulates protective
immunity in mice to influenza virus.
Vaccine 1994 Aug;12(11):1032–1040.
2. B Bender, CA Rowe, SF Taylor, LS
Wyatt, B Moss, PA Small Jr. Oral
immunization with a replicationdeficient recombinant vaccinia virus
protects mice against influenza. J Virol.
1996 Sep;70(9): 6418–6424.
Patent Status: HHS Reference No. E–
260–2009/0—Research Material. Patent
protection is not being sought for this
technology.
Related Technologies: HHS Reference
No. E–552–1982/2—
1. U.S. Patent No. 6,998,252 issued 14
Feb 2006, ‘‘Recombinant Poxviruses
Having Foreign DNA Expressed Under
the Control of Poxvirus Regulatory
Sequences’’
2. U.S. Patent No. 7,015,024 issued 21
Mar 2006, ‘‘Compositions Containing
Poxviruses Having Foreign DNA
Expressed Under the Control of
Poxvirus Sequences’’
3. U.S. Patent No. 7,045,313 issued 16
May 2006, ‘‘Recombinant Vaccinia
Virus Containing Chimeric Gene Having
Foreign DNA Flanked by Vaccinia
Regulatory DNA’’
4. U.S. Patent No. 7,045,136 issued 16
May 2006, ‘‘Methods of Immunization
Using Recombinant Poxviruses Having
Foreign DNA Expressed Under the
Control of Poxvirus Regulatory
Sequences’’
An abstract describing these
technologies may be viewed at https://
www.ott.nih.gov/Technologies/
abstractDetails.aspx?RefNo=2000.
Licensing Status: Available for
licensing.
Licensing Contacts: Uri Reichman,
Ph.D., MBA; 301–435–4616;
UR7a@nih.gov; RC Tang, JD, LLM; 301–
435–5031; tangrc@mail.nih.gov.
Biological/Research Material for HIV
Vaccine Research
Description of Technology: Offered for
licensing is a recombinant attenuated
vaccinia virus, MVA, that expresses SIV
239gagpol. The materials can be used
for research purposes and in particular
in the area of HIV/AIDS vaccines.
Plasmid insertion vector pJH–4,
containing the foreign gene SIV 239
GagPol controlled by vaccinia early/late
promoter, inserts into del III of
attenuated vaccinia MVA virus to make
recombinant MVA virus. The resulting
recombinant virus made from pJH4,
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16:37 Oct 15, 2009
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MVA/SIV239gagpol, expresses the SIV
239gagpol gene and thus can be used to
conduct vaccine studies in animal
models such as Rhesus macaques.
The list of publications shown below
demonstrates the usefulness of this
biological material in HIV vaccine
research.
Applications: Research reagents
useful in research and development in
the area of HIV/AIDS vaccines.
Development Status: Fully developed.
Material has been used extensively in
research.
Inventors: Bernard Moss and Linda S.
Wyatt (NIAID).
Publications:
1. RR Amara, F Villinger, JD Altman,
SL Lydy, SP O’Neil, SI Staprans, DC
Montefiori, Y Xu, JG Herndon, LS
Wyatt, MA Candido, NL Kozyr, PL Earl,
JM Smith, HL Ma, BD Grimm, ML
Hulsey, J Miller, HM McClure, JM
McNicholl, B Moss, HL Robinson.
Control of a mucosal challenge and
prevention of AIDS by a multiprotein
DNA/MVA vaccine. Science 2001 Apr
6;292(5514):69–74.
2. PL Earl, LS Wyatt, DC Montefiori,
M Bilska, R Woodward, PD Markbam,
JD Malley, TU Vogel, TM Allen, DI
Watkins, N Miller, B Moss. Comparison
of vaccine strategies using recombinant
env-gag-pol MVA with or without an
oligomeric Env protein boost in the
SHIV rhesus macaque model. Virology
2002 Mar 15;294(2):270–281.
3. RR Amara, JM Smith, SI Staprans,
DC Montefiori, F Villinger, JD Altman,
SP O’Neil, NL Kozyr, Y Xu, LS Wyatt,
PL Earl, JG Herndon, JM McNicholl, HM
McClure, B Moss, HL Robinson. Critical
role for Env as well as Gag-Pol in
control of a simian-human
immunodeficiency virus 89.6P
challenge by a DNA prime/recombinant
modified vaccinia virus Ankara vaccine.
J Virol. 2002 Jun;76(12):6138–6146.
4. RR Amara, F Villinger, SI Staprans,
JD Ahman, DC Montefiori, NL Kozyr, Y
Xu, LS Wyatt, PL Earl, JG Herndon, HM
McClure, B Moss, HL Robinson.
Different patterns of immune responses
but similar control of simian human
immunodeficiency virus 89.6P mucosal
challenge by modified vaccinia virus
Ankara (MVA) and DNA/MVA vaccines.
J Virol. 2002 Aug;76(15):7625–7631.
5. S Sadagopal, RR Amara, DC
Montefiori, LS Wyatt, SI Staprans, NL
Kozyr, HM McClure, B Moss, HL
Robinson. Signature for long-term
vaccine-mediated control of a Simian
and human immunodeficiency virus
89.6P challenge: stable low-breath and
low-frequency T-cell response capable
of coproducing gamma interferon and
interleukin-2. J Virol. 2005
Mar;79(6):3243–3253.
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Patent Status: HHS Reference No. E–
258–2009/0—Research Material. Patent
protection is not being pursued for this
technology.
Related Technologies: HHS Reference
No. E–552–1982/2—
1. U.S. Patent No. 6,998,252 issued 14
Feb 2006, ‘‘Recombinant Poxviruses
Having Foreign DNA Expressed Under
the Control of Poxvirus Regulatory
Sequences’’
2. U.S. Patent No. 7,015,024 issued 21
Mar 2006, ‘‘Compositions Containing
Poxviruses Having Foreign DNA
Expressed Under the Control of
Poxvirus Sequences’’
3. U.S. Patent No. 7,045,313 issued 16
May 2006, ‘‘Recombinant Vaccinia
Virus Containing Chimeric Gene Having
Foreign DNA Flanked by Vaccinia
Regulatory DNA’’
4. U.S. Patent No. 7,045,136 issued 16
May 2006, ‘‘Methods of Immunization
Using Recombinant Poxviruses Having
Foreign DNA Expressed Under the
Control of Poxvirus Regulatory
Sequences’’
An abstract describing these
technologies may be viewed at https://
www.ott.nih.gov/Technologies/
abstractDetails.aspx?RefNo=2000.
Licensing Status: Available for
licensing.
Licensing Contacts: Uri Reichman,
Ph.D., MBA; 301–435–4616;
UR7a@nih.gov; RC Tang, JD, LLM; 301–
435–5031; tangrc@mail.nih.gov.
A Target for the Development of
Diagnostics and Therapeutics for
Abnormal Hematopoiesis
Description of Technology: The zinc
finger protein ZFP36L2 has been shown
by the inventors to play an essential role
in hematopoiesis, a process that is
dysregulated in hematological cancers,
anemia, and other conditions. Thus,
ZFP36L2 has promise for use in a
diagnostic test to detect abnormal
hematopoiesis, or as a target for the
development of therapeutics to treat
abnormal hematopoiesis.
Hematopoiesis is the formation of
blood cellular components, through the
differentiation of hematopoietic stem
cells into lineages with a variety of
roles, such as carrying oxygen, immune
function, and blood clotting.
Abnormally high hematopoiesis can be
caused by hematological cancers such as
leukemia or lymphoma, or by other
myeloproliferative disorders.
Abnormally low hematopoiesis can be
caused by diseases such as anemia,
thrombocytopenia, or myelodysplastic
syndrome, and is often a secondary
symptom of other conditions, such as
cancer, infection, or dialysis.
E:\FR\FM\16OCN1.SGM
16OCN1
jlentini on DSKJ8SOYB1PROD with NOTICES
Federal Register / Vol. 74, No. 199 / Friday, October 16, 2009 / Notices
The inventors have discovered that
Zinc finger protein 36 like type-2
(ZFP36L2) plays an essential role in
hematopoiesis, possibly by affecting the
stability of mRNAs involved in this
process. ZFP36L2 is a member of the
tristetraprolin (TTP) family, which are
mRNA-binding proteins involved in
mRNA processing and degradation. The
invention discloses methods of
detecting abnormal hematopoiesis by
detecting abnormal ZFP36L2 expression
or a mutation in the ZFP36L2 gene, and
methods of controlling abnormal
hematopoiesis by modulating levels of
ZFP36L2 protein.
Applications:
• Diagnostic test to detect abnormal
hematopoiesis.
• Therapy for abnormal
hematopoiesis.
Development Status: Discovery stage.
Market:
• Over 3.5 million people in the
United States suffer from anemia,
according to NHLBI, and more than half
of all chemotherapy treatment for cancer
results in anemia.
• The American Cancer Society
estimates that approximately 4300 cases
of chronic myelogenous leukemia are
diagnosed in the United States every
year.
Inventors: Perry J. Blackshear and
Deborah J. Stumpo (NIEHS).
Related Publication: DJ Stumpo, HE
Broxmeyer, T Ward, S Cooper, G
Hangoc, YJ Chung, WC Shelley, EK
Richfield, MK Ray, MC Yoder, PD
Aplan, PJ Blackshear. Targeted
disruption of Zfp36l2, encoding a CCCH
tandem zinc finger RNA-binding
protein, results in defective
hematopoiesis. Blood 2009 Sep
17;114(12):2401–2410.
Patent Status: PCT Application Serial
No. PCT/US08/68900 filed on 01 Jul
2008 (HHS Reference No. E–255–2007/
0–PCT–02).
Licensing Status: Available for
licensing.
Licensing Contact: Tara Kirby, Ph.D.;
301–435–4426; tarak@mail.nih.gov.
Collaborative Research Opportunity:
The NIEHS Laboratory of Signal
Transduction, Polypeptide Hormone
Action Group, is seeking statements of
capability or interest from parties
interested in collaborative research to
further develop, evaluate, or
commercialize this technology. Please
contact Elizabeth M. Denholm, Ph.D.,
Director, Office of Technology Transfer,
NIEHS, at denholme@niehs.nih.gov for
more information.
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Susceptibility-Matched Multiwell Plates
for High-Throughput Screening by
Magnetic Resonance Imaging and
Nuclear Magnetic Resonance
Spectroscopy
Description of Technology: Available
for licensing and commercial
development is a patent estate that
covers multi-well assay plates for highthroughput screening by magnetic
resonance imaging (MRI) and nuclear
magnetic resonance (NMR)
spectroscopy. Multi-well plates are used
in a wide variety of high-throughput
measurements in clinical chemistry and
immunology, as well as in drug
discovery and other research
applications. Magnetic resonance
imaging (MRI) of multi-well plates offers
the possibility of performing new kinds
of high-throughput assays, including the
detection of magnetic nanoparticles
attached to or within cells. Moreover,
MRI-guided localized nuclear magnetic
resonance (NMR) spectroscopy could be
used to perform detailed chemical
analysis of complex mixtures of
metabolites not possible by any other
common analytical technique. Best of
all, conventional MRI techniques exist
which would permit all samples in one
or more multi-well plate(s) to be
analyzed simultaneously.
Unfortunately, conventional multi-well
plates typically give poor performance
for MRI-based assays since they provide
inadequate matching of magnetic
susceptibility between the plate, the
sample and their surroundings. This
results in distortion of the magnetic
field within the scanner and thus
reduces the sensitivity for detecting
magnetic particles and the resolution of
NMR spectra.
This invention relates to a new multiwell plate design incorporating onepiece polyetherimide plastic
construction for improved magnetic
susceptibility matching for aqueous
samples. This design can easily be
extended to non-aqueous samples by the
selection of an appropriate,
commercially available plastic resin or
resin blend. Further enhancement in
susceptibility matching can be
accomplished by combining the new
plate design with plugs for each well
constructed from the same plastic as the
plate. These plugs would allow the
entire thickness of each sample to be
scanned in chemical analyses,
improving signal-to-noise ratio and
sensitivity. These plugs can optionally
be integrated into a single ‘‘cap mat’’ so
that the entire assembly can be filled
and manipulated by standard robotic
laboratory equipment already in wide
use in the pharmaceutical industry.
PO 00000
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53267
Alternatively, spherical wells, accessed
by narrow fill holes, may be molded
into a solid plate, eliminating the need
for individual plugs to seal each well.
The new multi-well plate/plug design
reduces magnetic field distortions and
should dramatically improve spectral
resolution and sensitivity for NMR and
MRI-based high-throughput screening.
Applications:
• NMR Spectroscopy,
• MRI Imaging of magnetic
nanoparticles,
• Clinical Chemistry,
• Immunology,
• Drug Discovery,
• Combinatorial Chemistry, and
• Quality Control in the
pharmaceutical, chemical and
agricultural industries.
Advantages:
• Increased signal-to-noise ratio and
sensitivity relative to conventional
multi- well plates
• Portability
• Compatible with existing highthroughput robots.
Development Status: Used actively in
inventor’s lab.
Inventor: Kenneth W Fishbein (NIA).
Patent Status: U.S. Patent Application
No. 12/083,501 filed 30 Dec 2008 (HHS
Reference No. E–243–2005/0–US–03).
Licensing Status: Available for
licensing.
Licensing Contact: Michael
Shmilovich, Esq.; 301–435–5019;
shmilovm@mail.nih.gov.
Collaborative Research Opportunity:
The National Institute on Aging,
Magnetic Resonance Imaging &
Spectroscopy Section, is seeking
statements of capability or interest from
parties interested in collaborative
research to further develop, evaluate, or
commercialize this technology. Please
contact Nicole Darack, Ph.D. at 301–
435–3101 or darackn@mail.nih.gov for
more information.
Dated: October 5, 2009.
Richard U. Rodriguez,
Director, Division of Technology Development
and Transfer, Office of Technology Transfer,
National Institutes of Health.
[FR Doc. E9–24871 Filed 10–15–09; 8:45 am]
BILLING CODE 4140–01–P
DEPARTMENT OF HEALTH AND
HUMAN SERVICES
National Institutes of Health
Government-Owned Inventions;
Availability for Licensing
AGENCY: National Institutes of Health,
Public Health Service, HHS.
ACTION: Notice.
E:\FR\FM\16OCN1.SGM
16OCN1
Agencies
[Federal Register Volume 74, Number 199 (Friday, October 16, 2009)]
[Notices]
[Pages 53265-53267]
From the Federal Register Online via the Government Printing Office [www.gpo.gov]
[FR Doc No: E9-24871]
-----------------------------------------------------------------------
DEPARTMENT OF HEALTH AND HUMAN SERVICES
National Institutes of Health
Government-Owned Inventions; Availability for Licensing
AGENCY: National Institutes of Health, Public Health Service, HHS.
ACTION: Notice.
-----------------------------------------------------------------------
SUMMARY: The inventions listed below are owned by an agency of the U.S.
Government and are available for licensing in the U.S. in accordance
with 35 U.S.C. 207 to achieve expeditious commercialization of results
of federally-funded research and development. Foreign patent
applications are filed on selected inventions to extend market coverage
for companies and may also be available for licensing.
ADDRESSES: Licensing information and copies of the U.S. patent
applications listed below may be obtained by writing to the indicated
licensing contact at the Office of Technology Transfer, National
Institutes of Health, 6011 Executive Boulevard, Suite 325, Rockville,
Maryland 20852-3804; telephone: 301/496-7057; fax: 301/402-0220. A
signed Confidential Disclosure Agreement will be required to receive
copies of the patent applications.
Biological/Research Material for H1N1 Influenza Virus Vaccine Research
Description of Technology: Offered for licensing is a recombinant
attenuated vaccinia virus, MVA, that expresses the haemagglutinin (HA)
and nucleoprotein (NP) of influenza virus A/PR/8/34 (H1N1). The virus
has been shown to stimulate protective immunity to influenza virus in
mice.
The materials can be used for research purposes and in particular
in the area of influenza virus vaccines.
The related publications listed below demonstrate the usefulness of
this biological material in influenza virus vaccine research.
Applications: Research reagents useful in research and development
in the area of H1N1 Influenza virus vaccines.
Development Status: Fully developed. The usefulness of the
materials has been shown in Dr. Moss' laboratory.
[[Page 53266]]
Inventors: Bernard Moss and Linda S. Wyatt (NIAID).
Publications:
1. G Sutter, LS Wyatt, PL Foley, JR Bennink, B Moss. A recombinant
vector derived from the host range-restricted and highly attenuated MVA
strain of vaccinia virus stimulates protective immunity in mice to
influenza virus. Vaccine 1994 Aug;12(11):1032-1040.
2. B Bender, CA Rowe, SF Taylor, LS Wyatt, B Moss, PA Small Jr.
Oral immunization with a replication-deficient recombinant vaccinia
virus protects mice against influenza. J Virol. 1996 Sep;70(9): 6418-
6424.
Patent Status: HHS Reference No. E-260-2009/0--Research Material.
Patent protection is not being sought for this technology.
Related Technologies: HHS Reference No. E-552-1982/2--
1. U.S. Patent No. 6,998,252 issued 14 Feb 2006, ``Recombinant
Poxviruses Having Foreign DNA Expressed Under the Control of Poxvirus
Regulatory Sequences''
2. U.S. Patent No. 7,015,024 issued 21 Mar 2006, ``Compositions
Containing Poxviruses Having Foreign DNA Expressed Under the Control of
Poxvirus Sequences''
3. U.S. Patent No. 7,045,313 issued 16 May 2006, ``Recombinant
Vaccinia Virus Containing Chimeric Gene Having Foreign DNA Flanked by
Vaccinia Regulatory DNA''
4. U.S. Patent No. 7,045,136 issued 16 May 2006, ``Methods of
Immunization Using Recombinant Poxviruses Having Foreign DNA Expressed
Under the Control of Poxvirus Regulatory Sequences''
An abstract describing these technologies may be viewed at https://www.ott.nih.gov/Technologies/abstractDetails.aspx?RefNo=2000.
Licensing Status: Available for licensing.
Licensing Contacts: Uri Reichman, Ph.D., MBA; 301-435-4616;
UR7a@nih.gov; RC Tang, JD, LLM; 301-435-5031; tangrc@mail.nih.gov.
Biological/Research Material for HIV Vaccine Research
Description of Technology: Offered for licensing is a recombinant
attenuated vaccinia virus, MVA, that expresses SIV 239gagpol. The
materials can be used for research purposes and in particular in the
area of HIV/AIDS vaccines.
Plasmid insertion vector pJH-4, containing the foreign gene SIV 239
GagPol controlled by vaccinia early/late promoter, inserts into del III
of attenuated vaccinia MVA virus to make recombinant MVA virus. The
resulting recombinant virus made from pJH4, MVA/SIV239gagpol, expresses
the SIV 239gagpol gene and thus can be used to conduct vaccine studies
in animal models such as Rhesus macaques.
The list of publications shown below demonstrates the usefulness of
this biological material in HIV vaccine research.
Applications: Research reagents useful in research and development
in the area of HIV/AIDS vaccines.
Development Status: Fully developed. Material has been used
extensively in research.
Inventors: Bernard Moss and Linda S. Wyatt (NIAID).
Publications:
1. RR Amara, F Villinger, JD Altman, SL Lydy, SP O'Neil, SI
Staprans, DC Montefiori, Y Xu, JG Herndon, LS Wyatt, MA Candido, NL
Kozyr, PL Earl, JM Smith, HL Ma, BD Grimm, ML Hulsey, J Miller, HM
McClure, JM McNicholl, B Moss, HL Robinson. Control of a mucosal
challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine.
Science 2001 Apr 6;292(5514):69-74.
2. PL Earl, LS Wyatt, DC Montefiori, M Bilska, R Woodward, PD
Markbam, JD Malley, TU Vogel, TM Allen, DI Watkins, N Miller, B Moss.
Comparison of vaccine strategies using recombinant env-gag-pol MVA with
or without an oligomeric Env protein boost in the SHIV rhesus macaque
model. Virology 2002 Mar 15;294(2):270-281.
3. RR Amara, JM Smith, SI Staprans, DC Montefiori, F Villinger, JD
Altman, SP O'Neil, NL Kozyr, Y Xu, LS Wyatt, PL Earl, JG Herndon, JM
McNicholl, HM McClure, B Moss, HL Robinson. Critical role for Env as
well as Gag-Pol in control of a simian-human immunodeficiency virus
89.6P challenge by a DNA prime/recombinant modified vaccinia virus
Ankara vaccine. J Virol. 2002 Jun;76(12):6138-6146.
4. RR Amara, F Villinger, SI Staprans, JD Ahman, DC Montefiori, NL
Kozyr, Y Xu, LS Wyatt, PL Earl, JG Herndon, HM McClure, B Moss, HL
Robinson. Different patterns of immune responses but similar control of
simian human immunodeficiency virus 89.6P mucosal challenge by modified
vaccinia virus Ankara (MVA) and DNA/MVA vaccines. J Virol. 2002
Aug;76(15):7625-7631.
5. S Sadagopal, RR Amara, DC Montefiori, LS Wyatt, SI Staprans, NL
Kozyr, HM McClure, B Moss, HL Robinson. Signature for long-term
vaccine-mediated control of a Simian and human immunodeficiency virus
89.6P challenge: stable low-breath and low-frequency T-cell response
capable of coproducing gamma interferon and interleukin-2. J Virol.
2005 Mar;79(6):3243-3253.
Patent Status: HHS Reference No. E-258-2009/0--Research Material.
Patent protection is not being pursued for this technology.
Related Technologies: HHS Reference No. E-552-1982/2--
1. U.S. Patent No. 6,998,252 issued 14 Feb 2006, ``Recombinant
Poxviruses Having Foreign DNA Expressed Under the Control of Poxvirus
Regulatory Sequences''
2. U.S. Patent No. 7,015,024 issued 21 Mar 2006, ``Compositions
Containing Poxviruses Having Foreign DNA Expressed Under the Control of
Poxvirus Sequences''
3. U.S. Patent No. 7,045,313 issued 16 May 2006, ``Recombinant
Vaccinia Virus Containing Chimeric Gene Having Foreign DNA Flanked by
Vaccinia Regulatory DNA''
4. U.S. Patent No. 7,045,136 issued 16 May 2006, ``Methods of
Immunization Using Recombinant Poxviruses Having Foreign DNA Expressed
Under the Control of Poxvirus Regulatory Sequences''
An abstract describing these technologies may be viewed at https://www.ott.nih.gov/Technologies/abstractDetails.aspx?RefNo=2000.
Licensing Status: Available for licensing.
Licensing Contacts: Uri Reichman, Ph.D., MBA; 301-435-4616;
UR7a@nih.gov; RC Tang, JD, LLM; 301-435-5031; tangrc@mail.nih.gov.
A Target for the Development of Diagnostics and Therapeutics for
Abnormal Hematopoiesis
Description of Technology: The zinc finger protein ZFP36L2 has been
shown by the inventors to play an essential role in hematopoiesis, a
process that is dysregulated in hematological cancers, anemia, and
other conditions. Thus, ZFP36L2 has promise for use in a diagnostic
test to detect abnormal hematopoiesis, or as a target for the
development of therapeutics to treat abnormal hematopoiesis.
Hematopoiesis is the formation of blood cellular components,
through the differentiation of hematopoietic stem cells into lineages
with a variety of roles, such as carrying oxygen, immune function, and
blood clotting. Abnormally high hematopoiesis can be caused by
hematological cancers such as leukemia or lymphoma, or by other
myeloproliferative disorders. Abnormally low hematopoiesis can be
caused by diseases such as anemia, thrombocytopenia, or myelodysplastic
syndrome, and is often a secondary symptom of other conditions, such as
cancer, infection, or dialysis.
[[Page 53267]]
The inventors have discovered that Zinc finger protein 36 like
type-2 (ZFP36L2) plays an essential role in hematopoiesis, possibly by
affecting the stability of mRNAs involved in this process. ZFP36L2 is a
member of the tristetraprolin (TTP) family, which are mRNA-binding
proteins involved in mRNA processing and degradation. The invention
discloses methods of detecting abnormal hematopoiesis by detecting
abnormal ZFP36L2 expression or a mutation in the ZFP36L2 gene, and
methods of controlling abnormal hematopoiesis by modulating levels of
ZFP36L2 protein.
Applications:
Diagnostic test to detect abnormal hematopoiesis.
Therapy for abnormal hematopoiesis.
Development Status: Discovery stage.
Market:
Over 3.5 million people in the United States suffer from
anemia, according to NHLBI, and more than half of all chemotherapy
treatment for cancer results in anemia.
The American Cancer Society estimates that approximately
4300 cases of chronic myelogenous leukemia are diagnosed in the United
States every year.
Inventors: Perry J. Blackshear and Deborah J. Stumpo (NIEHS).
Related Publication: DJ Stumpo, HE Broxmeyer, T Ward, S Cooper, G
Hangoc, YJ Chung, WC Shelley, EK Richfield, MK Ray, MC Yoder, PD Aplan,
PJ Blackshear. Targeted disruption of Zfp36l2, encoding a CCCH tandem
zinc finger RNA-binding protein, results in defective hematopoiesis.
Blood 2009 Sep 17;114(12):2401-2410.
Patent Status: PCT Application Serial No. PCT/US08/68900 filed on
01 Jul 2008 (HHS Reference No. E-255-2007/0-PCT-02).
Licensing Status: Available for licensing.
Licensing Contact: Tara Kirby, Ph.D.; 301-435-4426;
tarak@mail.nih.gov.
Collaborative Research Opportunity: The NIEHS Laboratory of Signal
Transduction, Polypeptide Hormone Action Group, is seeking statements
of capability or interest from parties interested in collaborative
research to further develop, evaluate, or commercialize this
technology. Please contact Elizabeth M. Denholm, Ph.D., Director,
Office of Technology Transfer, NIEHS, at denholme@niehs.nih.gov for
more information.
Susceptibility-Matched Multiwell Plates for High-Throughput Screening
by Magnetic Resonance Imaging and Nuclear Magnetic Resonance
Spectroscopy
Description of Technology: Available for licensing and commercial
development is a patent estate that covers multi-well assay plates for
high-throughput screening by magnetic resonance imaging (MRI) and
nuclear magnetic resonance (NMR) spectroscopy. Multi-well plates are
used in a wide variety of high-throughput measurements in clinical
chemistry and immunology, as well as in drug discovery and other
research applications. Magnetic resonance imaging (MRI) of multi-well
plates offers the possibility of performing new kinds of high-
throughput assays, including the detection of magnetic nanoparticles
attached to or within cells. Moreover, MRI-guided localized nuclear
magnetic resonance (NMR) spectroscopy could be used to perform detailed
chemical analysis of complex mixtures of metabolites not possible by
any other common analytical technique. Best of all, conventional MRI
techniques exist which would permit all samples in one or more multi-
well plate(s) to be analyzed simultaneously. Unfortunately,
conventional multi-well plates typically give poor performance for MRI-
based assays since they provide inadequate matching of magnetic
susceptibility between the plate, the sample and their surroundings.
This results in distortion of the magnetic field within the scanner and
thus reduces the sensitivity for detecting magnetic particles and the
resolution of NMR spectra.
This invention relates to a new multi-well plate design
incorporating one-piece polyetherimide plastic construction for
improved magnetic susceptibility matching for aqueous samples. This
design can easily be extended to non-aqueous samples by the selection
of an appropriate, commercially available plastic resin or resin blend.
Further enhancement in susceptibility matching can be accomplished by
combining the new plate design with plugs for each well constructed
from the same plastic as the plate. These plugs would allow the entire
thickness of each sample to be scanned in chemical analyses, improving
signal-to-noise ratio and sensitivity. These plugs can optionally be
integrated into a single ``cap mat'' so that the entire assembly can be
filled and manipulated by standard robotic laboratory equipment already
in wide use in the pharmaceutical industry. Alternatively, spherical
wells, accessed by narrow fill holes, may be molded into a solid plate,
eliminating the need for individual plugs to seal each well. The new
multi-well plate/plug design reduces magnetic field distortions and
should dramatically improve spectral resolution and sensitivity for NMR
and MRI-based high-throughput screening.
Applications:
NMR Spectroscopy,
MRI Imaging of magnetic nanoparticles,
Clinical Chemistry,
Immunology,
Drug Discovery,
Combinatorial Chemistry, and
Quality Control in the pharmaceutical, chemical and
agricultural industries.
Advantages:
Increased signal-to-noise ratio and sensitivity relative
to conventional multi- well plates
Portability
Compatible with existing high-throughput robots.
Development Status: Used actively in inventor's lab.
Inventor: Kenneth W Fishbein (NIA).
Patent Status: U.S. Patent Application No. 12/083,501 filed 30 Dec
2008 (HHS Reference No. E-243-2005/0-US-03).
Licensing Status: Available for licensing.
Licensing Contact: Michael Shmilovich, Esq.; 301-435-5019;
shmilovm@mail.nih.gov.
Collaborative Research Opportunity: The National Institute on
Aging, Magnetic Resonance Imaging & Spectroscopy Section, is seeking
statements of capability or interest from parties interested in
collaborative research to further develop, evaluate, or commercialize
this technology. Please contact Nicole Darack, Ph.D. at 301-435-3101 or
darackn@mail.nih.gov for more information.
Dated: October 5, 2009.
Richard U. Rodriguez,
Director, Division of Technology Development and Transfer, Office of
Technology Transfer, National Institutes of Health.
[FR Doc. E9-24871 Filed 10-15-09; 8:45 am]
BILLING CODE 4140-01-P